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Redox Behavior of Copper A in Cytochrome Oxidase in the Brain In Vivo: Its Clinical Significance

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Oxygen Homeostasis and Its Dynamics

Part of the book series: Keio University Symposia for Life Science and Medicine ((KEIO,volume 1))

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Summary

The accuracy of near-infared measurement of the redox state of cytochrome oxidase in situ remains controversial. Our new approach to the measurement of the redox state of cytochrome oxidase resolves the most difficult problem, that the in vivo absorption coefficient of cytochrome oxidase is unknown, in addition to other problems such as the light-scattering effects and marked overlap of absorbance changes attributed to hemoglobin. We applied this method to both animal and clinical investigations. Based on the results obtained from these investigations, we discuss the redox behavior of cerebral cytochrome oxidase in vivo and the significance of the measurement of cytochrome oxidase in clinical medicine. Our conclusion is that cerebral cytochrome oxidase in vivo is fully oxidized under normal physiological conditions and that its oxygen-dependent redox change, which precedes a decline of brain function, occurs only when the oxygen supply is extremely impaired. Thus, the start of the reduction of cytochrome oxidase can be used as an alarm indicating that the brain condition is critical metabolically and functionally.

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References

  1. Jöbsis FF (1977) Noninvasive infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198: 1264–1267

    Article  PubMed  Google Scholar 

  2. Brazy JE, Lewis DV, Mitnick MH, Jöbsis FF (1985) Noninvasive monitoring of cerebral oxygenation in preterm infants: preliminary observations. Pediatrics 75: 217–225

    PubMed  CAS  Google Scholar 

  3. Skov L, Pryds O (1992) Capillary recruitment for presentation of cerebral glucose influx in hypoglycemic, preterm newborn: evidence for glucose sensor? Pediatrics 90: 193–195

    PubMed  CAS  Google Scholar 

  4. Wyatt JS, Cope M, Delpy DT, et al. (1986) Quantitation of cerebral oxygenation and haemodynamics in sick newborn infants by near infrared spectrophotometry. Lancet 2: 1063–1066

    Article  PubMed  CAS  Google Scholar 

  5. Chance B (1966) Spectrophotometric observations of absorbance changes in the infrared region in suspensions of mitochondria and in submitochondrial particles. In: Peisach G, Aisen P, Blumberg WE (eds) The biochemistry of copper. Academic, New York, pp 293–301

    Google Scholar 

  6. Ferrari M, Hanley DF, Wilson DA, Traystman RJ (1990) Redox changes in cat brain cytochrome-c oxidase after blood-fluorocarbon exchange. Am J Physiol 258: H1706 - H1713

    PubMed  CAS  Google Scholar 

  7. Jöbsis FF (1979) Oxidative metabolic effects of cerebral hypoxia. Adv Neurol 26: 299–318

    PubMed  Google Scholar 

  8. Wray S, Cope M, Delpy DT, et al. (1988) Characterization of the near-infared absorption spectra of cytochrome aa, and haemoglobin for the non-invasive monitoring of cerebral oxygenation. Biochim Biophys Acta 933: 184–192

    Article  PubMed  CAS  Google Scholar 

  9. Beinert HR, Shaw RW, Hansen RE, Hartzell CR (1980) Studies on the origin of near infrared (800–900nm) absorption of cytochrome c oxidase. Biochim Biophys Acta 591: 458–470

    Article  PubMed  CAS  Google Scholar 

  10. Hoshi Y, Hazeki O, Tamura M (1993) Oxygen dependence of redox state of copper in cytochrome oxidase in vitro. J Appl Physiol 74: 1622–1627

    PubMed  CAS  Google Scholar 

  11. Inagaki M, Tamura M (1993) Preparation and optical characteristics of hemoglobin-free isolated perfused rat head in situ. J Biochem (Tokyo) 113: 650–657

    CAS  Google Scholar 

  12. Hazeki O, Tamura M (1988) Quantitative analysis of hemoglobin oxygenation state of rat brain in situ by near-infrared spectrophotometry. J Appl Physiol 64: 796–802

    PubMed  CAS  Google Scholar 

  13. Matcher SJ, Elwell CE, Cooper CE, et al. (1995) Performance comparison of several published tissue near-infrared spectroscopy algorithms. Anal Biochem 227: 54–68

    Article  PubMed  CAS  Google Scholar 

  14. Jöbsis FF, Keizer JH, LaManna JC, Rothenthal M (1977) Reflectance spectrophotometry of cytochrome aa3 in vivo. J Appl Physiol 43: 858–872

    PubMed  Google Scholar 

  15. Hempel FG, Kariman K, Saltzman HA (1980) Redox transitions in mitochondria of cat cerebral cortex with seizures and hemorrhagic hypotension. Am J Physiol 238: H249 - H256

    PubMed  CAS  Google Scholar 

  16. Ferrari M, Williams MA, Wilson DA, et al. (1995) Cat brain cytichrome-c oxidase redox changes induced by hypoxia after blood-fluorocarbon exchange transfusion. Am J Physiol 269: H417 - H424

    PubMed  CAS  Google Scholar 

  17. Sylvia AL, Piantadosi CA (1988) O2 dependece of in vivo brain cytochrome redox responses and energy metabolism in bloodless rats. J Cereb Blood Flow Metab 8: 163–172

    Article  PubMed  CAS  Google Scholar 

  18. Hoshi Y, Tamura M (1993) Dynamic changes in cerebral oxygenation in chemically induced seizures in rat: study by near-infrared spectrophotometry. Brain Res 603: 215–221

    Article  PubMed  CAS  Google Scholar 

  19. Cooper CE, Matcher SJ, Wyatt JS, et al. (1994) Near-infrared spectroscopy of the brain: relevance to cytochrome oxidase bioenergetics. Biochim Soc Trans 22: 974–980

    CAS  Google Scholar 

  20. Tsuji M, Naruse H, Volpe J, Holtzman D (1995) Reduction of cytochrome aa3 measured by near-infred spectroscopy predicts cerebral energy loss in hypoxic piglets. Pediatr Res 37: 253–259

    Article  PubMed  CAS  Google Scholar 

  21. Edwards AD, Brown GC, Cope M, et al. (1991) Quantitation of concentration changes in neonatal human cerebral oxidized cytochrome oxidase. J Appl Physiol 71: 1907–1911

    PubMed  CAS  Google Scholar 

  22. Sugano T, Oshino N, Chance B (1974) Mitochodrial functions under hypoxic conditions. The steady states of cytochrome c reduction and of energy metabolism. Biochim Biophys Acta 347: 340–356

    Article  PubMed  CAS  Google Scholar 

  23. Tamura M, Hazeki O, Nioka S, et al. (1988) The simultaneous measurements of tissue oxygen concentration and energy state by near-infrared and nuclear magnetic resonance spectroscopy. In: Lahiri S (ed) Chemoreceptors and reflexes in breathing. Oxford, New York, pp 91–93

    Google Scholar 

  24. Ginsburg DA, Pasternak EB, Gurvitch AM (1977) Correlation analysis of delta activity generated in cerebral hypoxia. Electroencephalogr Clin Neurophysiol 42: 445–455

    Article  PubMed  CAS  Google Scholar 

  25. van der Zee P, Cope M, Arridge SR, et al. (1992) Experimentally measured optical pathlengths for the adult head, calf and forearm and the head of the newborn infant as a function of inter optode spacing. Adv Exp Med Biol 16: 143–153

    Google Scholar 

  26. Obrig H, Hirth C, Döge C, et al. (1995) Assesment of localized changes in hemoglobin and cytochrome-oxidase oxidation during performance of a motor task. J Cereb Blood Flow Metab 15 (suppl 1): S78

    Article  Google Scholar 

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Authors and Affiliations

  1. Biophysics Group, Research Institue for Electronic Science, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo 060, Japan

    Yoko Hoshi, Osamu Hazeki, Yasutomo Nomura, Yasuyuki Kakihana & Mamoru Tamura

  2. New Energy and Industrial Technology Development Organization, Tokyo 170, Japan

    Yoko Hoshi

  3. Shimadzu Corporation Technology Research Laboratory, Hadano, Kanagawa, 259-13, Japan

    Hideo Eda

  4. Department of Anestheology, School of Medicine, Kagoshima University, Kagoshima 890, Japan

    Yasuyuki Kakihana

  5. Department of Neurosurgery, School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo 060, Japan

    Satoshi Kuroda

Authors
  1. Yoko Hoshi
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  2. Hideo Eda
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  3. Osamu Hazeki
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  4. Yasutomo Nomura
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  5. Yasuyuki Kakihana
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  6. Satoshi Kuroda
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  7. Mamoru Tamura
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

    Yuzuru Ishimura M.D., Ph.D. (Professor and Chairman), Hideo Shimada Ph.D. (Associate Professor) & Makoto Suematsu M.D., Ph.D. (Associate Professor) (Professor and Chairman),  (Associate Professor) &  (Associate Professor)

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© 1998 Springer-Verlag Tokyo

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Hoshi, Y. et al. (1998). Redox Behavior of Copper A in Cytochrome Oxidase in the Brain In Vivo: Its Clinical Significance. In: Ishimura, Y., Shimada, H., Suematsu, M. (eds) Oxygen Homeostasis and Its Dynamics. Keio University Symposia for Life Science and Medicine, vol 1. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68476-3_9

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  • DOI: https://doi.org/10.1007/978-4-431-68476-3_9

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68478-7

  • Online ISBN: 978-4-431-68476-3

  • eBook Packages: Springer Book Archive

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