Summary
The role of the transcription factor, nuclear factor NF-κB, in the induction of cytokines and intercellular adhesion molecule-1 (ICAM-1) on stimulation with interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) was studied in primary rheumatoid synovial fibroblasts (RSF) obtained from patients with rheumatoid arthritis (RA). The production of GM-CSF, IL-6, and IL-8 and the expression of ICAM1 were augmented after nuclear translocation of NF-κB induced by treatment with IL-1 or TNF-α. We examined the effects of N-acetyl-l-cysteine (NAC) and acetylsalicylic acid (aspirin) on the induction of proinflammatory cytokines and ICAM-1. Pretreatment of RSF with NAC inhibited nuclear translocation of NF-κB completely, and the induction of these cytokines and ICAM-1 was markedly suppressed. On the other hand, the effect of aspirin was only partial. These observations indicate the pivotal role of NF-κB in RA pathogenesis, thus highlighting the possibility of a novel therapeutic strategy.
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References
Alvaro-Gracia JM, Zvaifler NJ, Brown CB, et al. (1991) Cytokines in chronic arthritis. VI. Analysis of the synovial cells involved in granulocyte macrophage colony-stimulating factor production and gene expression in rheumatoid arthritis and its regulation by IL-1 and TNF-a. J Immunol 146: 3365–3371
Baeuerle PA (1991) The inducible transcription activator NF-KB: regulation by distinct protein subunits. Biochim Biophys Acta 1072: 63–80
Wickes IP, Leizer T, Wawryk S, et al. (1992) The effect of cytokines on the expression of MHC antigens and ICAM-1 by normal and transformed synoviocytes. Autoimmunity 12: 13–19
Weber C, Erl W, Pietsh A, et al. (1995) Aspirin inhibits nuclear factor-KB mobilization and monocyte adhesion in stimulated human endothelial cells. Circulation 91: 1914
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© 1998 Springer-Verlag Tokyo
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Sakurada, S., Kato, T., Mashiba, K., Jian-Ping, Y., Okamoto, T. (1998). Inhibition of Cytokines and ICAM-1 Induction in Rheumatoid Fibroblasts by anti-NF-κB Reagents. In: Ishimura, Y., Shimada, H., Suematsu, M. (eds) Oxygen Homeostasis and Its Dynamics. Keio University Symposia for Life Science and Medicine, vol 1. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68476-3_57
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DOI: https://doi.org/10.1007/978-4-431-68476-3_57
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68478-7
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