Summary
In our study, we found that the Ca2+ antagonist nilvadipine was beneficial for the preservation of photoreceptor cells in The Royal College of Surgeons (RCS) rats. Here, in order to elucidate mechanisms of its nilvadipine-induced photoreceptor preservation, we analyzed altered gene expressions of the retina in RCS rats administered nilvadipine by mRNA profiling assay. Total RNA isolated from the retina with or without nilvadipine was converted into cDNA. Utilizing DNA microarray analysis methods, we compared the overall expression patterns for 1101 genes that were commonly expressed in rodent. Of the total genes, the expression of less than 30 genes was altered significantly including that of several genes involved in cellular regulation. On the basis of these data, it is suggested that microarray analysis is a useful tool and applicable for studying the pharmacological effects of several drugs including Ca2+ channel blockers to retinal degeneration.
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© 2003 Springer-Verlag Tokyo
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Ishikawa, F. et al. (2003). Pharmacological Aspects of Nilvadipine-Induced Preservation of Retinal Degeneration in RCS Rat Analyzed by mRNA Profiling Assay. In: Kaneko, A. (eds) The Neural Basis of Early Vision. Keio University International Symposia for Life Sciences and Medicine, vol 11. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68447-3_61
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DOI: https://doi.org/10.1007/978-4-431-68447-3_61
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68449-7
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