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SN1 Catalyzes Transport of Glutamine Across Müller Cells in Retina

  • Kyoh-Ichi Takahashi
  • David Krizaj
  • David R. Copenhagen
Conference paper
  • 146 Downloads
Part of the Keio University International Symposia for Life Sciences and Medicine book series (KEIO, volume 11)

Abstract

High-affinity glutamate (Glu) transporters localized on Müller cell membranes transport synaptically released Glu into these cells from the extracellular side. Müller cells, similar to most glial cells in the nervous system, generate glutamine (Gln) from Glu using the enzyme glutamine synthetase. Although it has been postulated that Gln is transported from these Müller cells to replenish synaptic Glu, little is known about the identity or properties of the Gln transporter. We investigated the Gln transport mechanism in Müller cells dissociated from retinae of tiger salamander (Ambystoma tigrinum) using three different approaches: intracellular pH (pHi) measurements, whole-cell voltage-clamp, and immunocytochemistry.

Key words

Müller cell Glutamine transport Glutamate/glutamine cycle 

Reference

  1. 1.
    Chaudhry FA, Reimer RJ, Krizaj D, et al. (1999) Molecular analysis of System N suggests novel physiological roles in nitrogen metabolism and synaptic transmission, Cell 99: 769–780PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 2003

Authors and Affiliations

  • Kyoh-Ichi Takahashi
    • 1
    • 2
  • David Krizaj
    • 2
  • David R. Copenhagen
    • 2
  1. 1.Division of Biological Sciences, Faculty of Human Environmental StudiesHiroshima Shudo UniversityAsaminami-ku, HiroshimaJapan
  2. 2.Departments of Ophthalmology and PhysiologyUCSF School of MedicineSan FranciscoUSA

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