The Effect of Centrifugal Force on Glycosaminoglycans Synthesis and Cell Proliferation of Vascular Smooth Muscle Cells in Culture
To evaluate the role of hypertension independent of humoral factors on atherogenesis, synthesis of glycosaminoglycans (GAGs), protein and DNA were tested in the cultured vascular smooth muscle cells(NSMCs) from aortae of spontaneously hypertensive rats(SHR) and Wistar-Kyoto rats(WKY) under centrifugal force. Basal levels of GAGs and DNA were greater in SHR compared with WKY. Under the centrifugal force, cultured VSMCs synthesized more GAGs in both strains. DNA and protein syntheses were more suppressed by centrifugal force in WKY than in SHR. These results suggest that high blood pressure per se might promote atherosclerosis via metabolic changes in VSMCs, especially in genetic hypertensive animals.
KeywordsVascular Smooth Muscle Cell Centrifugal Force Thymidine Incorporation Mucopoly Saccharide Gravity Level
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