The Effect of a Fluid-Imposed Shear Stress on the Mechanical Properties of Cultured Endothelial Cells

  • M. Sato
  • M. J. Levesque
  • R. M. Nerem


The mechanical properties of cultured bovine aortic endothelial cells exposed to a fluid-imposed shear stress were studied using the micropipette technique. The cells, which were attached to a Thermanox plastic substrate, were exposed to a specific steady or pulsatile shear stress. The cells exposed to shear stress demonstrated a mechanical stiffness which was significantly larger than those of control cells, being dependent both on the level of shear stress and the duration of exposure. The cells exposed to pulsatile shear stress became much stiffer in their mechanical property than those exposed to steady shear stress. This effect was particularly dramatic in the case of high shear stress.


Shear Stress Flow Chamber High Shear Stress Stiffness Parameter Bovine Aortic Endothelial Cell 


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  1. [1]
    Nerem RM, Cornhill JF (1980) J Biomech Eng 102: 181–189PubMedCrossRefGoogle Scholar
  2. [2]
    Ross R (1981) Arteriosclerosis 1: 293–311PubMedCrossRefGoogle Scholar
  3. [3]
    Flaherty JT, Pierce JE, Ferrans VJ, Patel DJ, Tucker WK, Fry DL (1972) Circ Res 31: 23–33Google Scholar
  4. [4]
    Nerem RM, Levesque MJ, Cornhill JF (1981) J Biomech Eng 103: 172–176PubMedCrossRefGoogle Scholar
  5. [5]
    Dewey CF, Bussolari SR, Gimbrone MA Jr, Davies PF (1981) J Biomech Eng 103: 177–185PubMedCrossRefGoogle Scholar
  6. [6]
    Levesque MJ, Nerem RM (1985) J Biomech Eng 107: 341–347PubMedCrossRefGoogle Scholar
  7. [7]
    Silkworth JB, Stehbens W (1975) Angiology 26: 474–487CrossRefGoogle Scholar
  8. [8]
    Sato M, Levesque MJ, Nerem RM (1987) J Biomech Eng 109: 27–34PubMedCrossRefGoogle Scholar
  9. [9]
    Sato M, Levesque MJ, Nerem RM (1987) Arteriosclerosis 7: 276–286PubMedCrossRefGoogle Scholar
  10. [10]
    Levesque MJ, Sato M, Nerem RM (to be published)Google Scholar

Copyright information

© Springer-Verlag Tokyo 1988

Authors and Affiliations

  • M. Sato
    • 1
  • M. J. Levesque
    • 2
  • R. M. Nerem
    • 2
  1. 1.Institute of Basic Medical SciencesUniversity of TsukubaTsukuba, 305Japan
  2. 2.Biomechanics Laboratory and School of Mechanical EngineeringGeorgia Institute of TechnologyAtlantaUSA

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