The Effect of a Fluid-Imposed Shear Stress on the Mechanical Properties of Cultured Endothelial Cells

  • M. Sato
  • M. J. Levesque
  • R. M. Nerem


The mechanical properties of cultured bovine aortic endothelial cells exposed to a fluid-imposed shear stress were studied using the micropipette technique. The cells, which were attached to a Thermanox plastic substrate, were exposed to a specific steady or pulsatile shear stress. The cells exposed to shear stress demonstrated a mechanical stiffness which was significantly larger than those of control cells, being dependent both on the level of shear stress and the duration of exposure. The cells exposed to pulsatile shear stress became much stiffer in their mechanical property than those exposed to steady shear stress. This effect was particularly dramatic in the case of high shear stress.


Shear Stress Flow Chamber High Shear Stress Stiffness Parameter Bovine Aortic Endothelial Cell 
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Copyright information

© Springer-Verlag Tokyo 1988

Authors and Affiliations

  • M. Sato
    • 1
  • M. J. Levesque
    • 2
  • R. M. Nerem
    • 2
  1. 1.Institute of Basic Medical SciencesUniversity of TsukubaTsukuba, 305Japan
  2. 2.Biomechanics Laboratory and School of Mechanical EngineeringGeorgia Institute of TechnologyAtlantaUSA

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