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Manipulation of Genes Involved in Sulfur and Glutathione Metabolism

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Air Pollution and Plant Biotechnology

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Abstract

Sulfur is an essential nutrient for plant growth and development and, under conditions of sufficient sulfur availability, is taken up from the soil by roots in the form of sulfate and transferred to the shoots. The sulfate is subsequently activated and then reduced to sulfite and then to sulfide, in what is generally referred to as the sulfur reduction assimilatory pathway; the main function and ultimate product of which is the biosynthesis of cysteine (Cys). The Cys thus formed serves as a precursor for several other reduced sulfur-containing metabolites, notably methionine and glutathione (GSH), the latter constituting the principal storage and transport form of reduced sulfur in plants, as well as a key factor controlling plant responses to a variety of biotic and abiotic stresses (Noctor et al. 1998a; May et al. 1998a).

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References

  • Arisi ACM, Noctor G, Foyer C, Jouanin L (1997) Modifications of thiol contents in poplars (poplar tremula x P. alba) overexpressing enzymes involved in glutathione synthesis. Planta 203: 362–372

    Article  PubMed  CAS  Google Scholar 

  • Barroso C, Romero LC, Cejudo FJ, Vega JM, Gotor C (1999) Salt-specific regulation of the cytosolic O-acetylserine(thiol)lyase gene from Arabidopsis is dependent on abscisic acid. Plant Mol Biol 40: 729–736

    Article  PubMed  CAS  Google Scholar 

  • Bergmann L, Rennenberg H (1993) Glutathione metabolism in plants. In: De Kok LJ, Stulen I, Rennenberg H et al. (eds) Sulphur nutrition and assimilation in higher plants. SPB Academic, The Hague, pp 107–123

    Google Scholar 

  • Bick J A, Leustek T (1998) Plant sulfur metabolism: the reduction of sulfate to sulfite. Curr Opin Plant Biol 1: 240–244

    Article  PubMed  CAS  Google Scholar 

  • Blaszczyk A, Brodzik R, Sirko A (1999) Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase. Plant J 20: 237–243

    Article  PubMed  CAS  Google Scholar 

  • Bogdanova N, Bork C, Hell R (1995) Cysteine biosynthesis in plants: isolation and functional characterization of a cDNA encoding serine acetyltransferase from Arabidopsis thaliana. FEBS Lett 358: 43–47

    Article  PubMed  CAS  Google Scholar 

  • Bolchi A, Petrucco S, Tenca PL, Foroni C, Ottonello S (1999) Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status: stereospecific down-regulation by L-cysteine. Plant Mol Biol 39: 527–537

    Article  PubMed  CAS  Google Scholar 

  • Bork C, Schwenn JD, Hell R (1998) Isolation and characterization of a gene for assimilatory sulfite reductase from Arabidopsis thaliana. Gene 212: 147–153

    Article  PubMed  CAS  Google Scholar 

  • Bowler C, Van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43: 83–116

    Article  CAS  Google Scholar 

  • Brunold C, Rennenberg H (1997) Regulation of sulphur metabolism in plants: first molecular approaches. Prog Bot 58: 164–186

    CAS  Google Scholar 

  • Creissen G, Broadbent P, Stevens R, Wellburn AR, Mullineaux P (1996) Manipulation of glutathione metabolism in transgenic plants. Biochem Soc Trans 24: 465–469

    PubMed  CAS  Google Scholar 

  • Creissen G, Firmin J, Fryer M, Kular B, Leyland N, Reynolds H, Pastori G, Wellburn F, Baker N, Wellburn A, Mullineaux P (1999) Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell 11: 1277–1291

    Article  PubMed  CAS  Google Scholar 

  • De Kok LJ (1990) Sulfur metabolism in plants exposed to atmospheric sulphur. In: Rennenberg H, Brunold C, De Kok LJ, Stulen I (eds) Sulfur nutrition and sulfur assimilation in higher plants. SPB Academic, The Hague, pp 111–130

    Google Scholar 

  • Droux M, Ruffet ML, Douce R, Job D (1998) Interactions between serine acetyltransferase and 0-acetylserine (thiol) lyase in higher plants: structural and kinetic properties of the free and bound enzymes. Eur J Biochem 255: 235–245

    Article  PubMed  CAS  Google Scholar 

  • Foyer CH, Souriau N, Perret S, Lelandais M, Kunert K-J, Pruvost C, Jouanin L (1995) Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109: 1047–1057

    Article  PubMed  CAS  Google Scholar 

  • Frendo P, Gallessi D, Turnbull R, Van de Sype G, Hérouart D, Puppo A (1999) Localization of glutathione and homoglutathione in Medicago trunculata is correlated to a differential expression of genes involved in their synthesis. Plant J 17: 215–219

    Article  CAS  Google Scholar 

  • Genty BE, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92

    Article  CAS  Google Scholar 

  • Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL (1996) Three members of a novel small gene family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and “APS reductase” activity. Proc Natl Acad Sci USA 93: 13377–13382

    Article  PubMed  CAS  Google Scholar 

  • Hatzfeld Y, Cathala N, Grignon C, Davidian J-C (1998) Effect of ATP sulfurylase overexpression in Bright Yellow 2 tobacco cells. Regulation of ATP sulfurylase and SO42- transport activities. Plant Physiol 116: 1307–1313

    Article  PubMed  CAS  Google Scholar 

  • Hell R (1997) Molecular physiology of plant sulphur metabolism. Planta 202: 138–148

    Article  PubMed  CAS  Google Scholar 

  • Hell R, Bergman L (1990) γ-Glutamylcysteine synthase in higher plants: catalytic properties and subcellular localization. Planta 180: 603–612

    Google Scholar 

  • Hérouart D, Van Montagu M, Inze D (1993) Redox-activated expression of the cytosolic copper/zinc superoxide dismutase gene in Nicotiana. Proc Natl Acad Sci USA 90: 3108–3112

    Article  PubMed  Google Scholar 

  • Klonus D, Hofgen R, Willmitzer L, Riesmeier JW (1994) Isolation and characterization of two cDNA clones encoding ATP-sulfurylases from potato by complementation of a yeast mutant. Plant J 6: 105–112

    Article  PubMed  CAS  Google Scholar 

  • Klonus D, Riesmeier JW, Willmitzer L (1995) A cDNA clone for an ATP-sulfurylase from Arabidopsis thaliana. Plant Physiol 107: 653–654

    Article  PubMed  CAS  Google Scholar 

  • Kopriva S, Muheim R, Koprivova A, Trachsel N, Catalano C, Suter M, Brunold C (1999) Light regulation of assimilatory sulphate reduction in Arabidopsis thaliana. Plant J 20: 37–44

    Article  PubMed  CAS  Google Scholar 

  • Lappartient AG, Vidmar JJ, Leustek T, Glass ADM, Touraine B (1999) Inter-organ signalling in plants: regulation of ATP-sulfurylase and sulfate transporter genes expression in roots mediated by phloem-translocated compound. Plant J 18: 89–95

    Article  PubMed  CAS  Google Scholar 

  • Lee S, Leustek T (1999) The effect of cadmium on sulfate assimilation enzymes in Brassica juncea. Plant Sci 141: 201–207

    Article  CAS  Google Scholar 

  • Leustek T (1996) Molecular genetics of sulfate assimilation in plants. Physiol Plant 97: 411–419

    Article  CAS  Google Scholar 

  • Leustek T, Saito K (1999) Sulphate transport and assimilation in plants. Plant Physiol 120: 637–643

    Article  PubMed  CAS  Google Scholar 

  • Leustek T, Murillo M, Cervantes M (1994) Cloning of a cDNA encoding ATP sulfurylase from Arabidopsis thaliana by functional complementation in Saccharomyces cerevisae. Plant Physiol 105: 897–902

    Article  PubMed  CAS  Google Scholar 

  • Lunn JE, Droux M, Martin J, Douce R (1990) Localization of ATP sulfurylase and O-acetyl-L-serine(thiol)lyase in spinach leaves. Plant Physiol 94: 1345–1352

    Article  PubMed  CAS  Google Scholar 

  • Madamanchi NR, Alscher RG (1991) Metabolic bases for differences in sensitivity of two pea cultivars to sulfur dioxide. Plant Physiol 97: 88–93

    Article  PubMed  CAS  Google Scholar 

  • Maruyama A, Ishizawa K, Takagi T, Esashi Y (1998) Cytosolic β-cyanoalanine synthase activity attributed to cysteine synthases in cocklebur seeds. Purification and characterization of cytosolic cysteine synthases. Plant Cell Physiol 39: 671–680

    PubMed  CAS  Google Scholar 

  • May MJ, Leaver CJ (1994) Arabidopsis thaliana γ-glutamylcysteine synthase is structurally unrelated to mammalian, yeast and E. coli homologs. Proc Natl Acad Sci USA 91: 10059–10063

    Article  PubMed  CAS  Google Scholar 

  • May MJ, Vernoux T, Leaver C, Van Montagu M, Inze D (1998a) Glutathione homeostasis in plants: implications for environmental sensing and plant development. J Exp Bot 49: 649–667

    Article  CAS  Google Scholar 

  • May MJ, Vernoux T, Sanchez-Fernandez R, Van Montagu M, Inze D (1998b) Evidence for posttranscriptional activation of γ-glutamylcysteine synthase during plant stress responses. Proc Natl Acad Sci USA 95: 12049–12054

    Article  PubMed  CAS  Google Scholar 

  • Nakamura T, Yamaguchi Y, Sano H (1999) Four rice genes encoding cysteine synthase: isolation and differential responses to sulfur, nitrogen and light. Gene 229: 155–161

    Article  PubMed  CAS  Google Scholar 

  • Noctor G, Strohm, M, Jouanin L, Kunert KJ, Foyer CH, Rennenberg H (1996) Synthesis of glutathione in leaves of transgenic poplar (Populus tremula x P. alba) overexpressing γ-glutamylcysteine synthetase. Plant Physiol 112: 1071–1078

    PubMed  CAS  Google Scholar 

  • Noctor G, Jouanin L, Foyer CH (1997) The biosynthesis of glutathione explored in transgenic plants. In: Hatzios K (ed) Regulation of enzymatic systems detoxifying xenobiotics in plants. Nato ASI series. Kluwer, Dordrecht, pp 109–124

    Google Scholar 

  • Noctor G, Arisi ACM, Jouanin L, Kunert KJ, Rennenberg H, Foyer CH (1998a) Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J Exp Bot 49: 623–647

    Article  CAS  Google Scholar 

  • Noctor G, Arisi ACM, Jouanin L, Foyer CH (1998b) Manipulation of glutathione and amino acid biosynthesis in the chloroplast. Plant Physiol 118: 471–482

    Article  PubMed  CAS  Google Scholar 

  • Pilon-Smits EHA, Hwang S, Lytle CM, Zhu Y, Tai JC, Bravo RC, Chen Y, Leustek T, Terry N (1999) Overexpression of ATP sulfurylase in Indian mustard leads to increased selenate uptake, reduction and tolerance. Plant Physiol 119: 123–132

    Article  PubMed  CAS  Google Scholar 

  • Rawlins MR, Leaver CJ, May MJ (1995) Characterization of a cDNA encoding Arabidopsis glutathione synthetase. FEBS Lett 376: 81–86

    Article  PubMed  CAS  Google Scholar 

  • Rennenberg H (1984) The fate of excess sulphur in higher plants. Annu Rev Plant Physiol 35: 121–153

    Article  CAS  Google Scholar 

  • Rennenberg H (1997) Molecular approaches to glutathione biosynthesis. In: Cram WJ, De Kok LJ, Stulen I, et al. (eds) Sulphur metabolism in higher plants, molecular, ecophysiological and nutritional aspects. Backhuys, Leiden, pp 59–70

    Google Scholar 

  • Rennenberg H, Herschbach C (1996) Responses of plants to atmospheric sulphur. In: Yunus M, Iqbal M (eds) Plant response to air pollution. J Wiley, London, pp 285–293

    Google Scholar 

  • Rolland N, Droux M, Douce R (1992) Subcellular distribution of O-acetylserine(thiol)lyase in cauliflower (Brassica oleracea L.) inflorescence. Plant Physiol 98: 927–935

    Article  PubMed  CAS  Google Scholar 

  • Ruffet M-L, Droux M, Douce R (1994) Purification and kinetic properties of serine acetyl transferase free of 0-acetylserine(thiol)lyase from spinach chloroplasts. Plant Physiol 104: 597–604

    PubMed  CAS  Google Scholar 

  • Saito K, Kurosawa M, Tatsuguchi K, Takagi Y, Murakoshi I (1994) Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O- acetylserine(thiol)-lyase]. Plant Physiol 106: 887–895

    Article  PubMed  CAS  Google Scholar 

  • Schäfer HJ, Greiner S, Rausch T, Haag-Kerwer A (1997) In seedlings of the heavy metal accumulator Brassica juncea Cu2+ differentially affects transcript amounts for γ-glutamylcysteine synthase (γ-ECS) and metallothionein (MT2). FEBS Lett 404: 216–220

    Article  PubMed  Google Scholar 

  • Schäfer HJ, Haag-Kerwer A, Rausch T (1998) cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L. evidence for Cd-induction of a putative mitochondrial γ-glutamylcysteine synthase isoform. Plant Mol Biol 37: 87–97

    Google Scholar 

  • Schnug E (1998) Response of plant metabolism to air pollution and global change - impact on agriculture. In: De Kok LJ, Stulen I (eds) Responses of plant metabolism to air pollution and global change. Backhuys, Leiden, pp 15–22

    Google Scholar 

  • Sen Gupta A, Alscher RG, McCune D (1991) Response of photosynthesis and cellular antioxidants to ozone in Populus leaves. Plant Physiol 96: 650–655

    Article  CAS  Google Scholar 

  • Shimazaki K-I, Sakaki T, Kondo N, Sugahara K (1980) Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2-fumigated leaves of spinach. Plant Cell Physiol 21: 1193–1204

    CAS  Google Scholar 

  • Smith FW, Ealing PM, Hawkesford MJ, Clarkson DT (1995) Plant members of a family of sulphate transporters reveal functional subtypes. Proc Natl Acad Sci USA 92: 9373–9377

    Article  PubMed  CAS  Google Scholar 

  • Smith FW, Hawkesford MJ, Ealing PM, Clarkson DT, Vanden Berg PJ, Belcher AR, Warrilow AGS (1997) Regulation of expression of a cDNA encoding a high affinity sulphate transporter. Plant J 12: 875–884

    Article  PubMed  CAS  Google Scholar 

  • Strohm M, Jouanin L, Kunert, KJ, Pruvost C, Polle A, Foyer CH, Rennenberg H (1995) Regulation of glutathione synthesis in leaves of transgenic poplar (Populus tremula x P. alba) overexpressing glutathione synthetase. Plant J 7: 141–145

    Article  CAS  Google Scholar 

  • Strohm M, Eiblmeier M, Langebartels C, Jouanin L, Polle A, Sandermann H, Rennenberg H (1999) Responses of transgenic poplar (Populus tremula x P. alba) overexpressing glutathione synthetase or glutathione reductase to acute ozone stress: visible injury and leaf gas exchange. J Exp Bot 50: 363–372

    Article  Google Scholar 

  • Takahashi H, Saito K (1996) Subcellular localization of spinach cysteine synthase isoforms and regulation of their gene expression by nitrogen and sulfur. Plant Physiol 112: 273–280

    Article  PubMed  CAS  Google Scholar 

  • Takahashi H, Yamazaki M, Sasakura N, Watanabe A, Leustek T, de Almeida Engler J, Engler G, Van Montagu M, Saito K (1997) Regulation of sulphur assimilation in higher plants: a sulphur transporter induced in sulfate-starved roots plays a central role in Arabidopsis thaliana. Proc Natl Acad Sci USA 94: 11102–11107

    Article  PubMed  CAS  Google Scholar 

  • Wellburn AR (1988) Air pollution and acid rain: the biological impact. Longman, England, pp 1–274

    Google Scholar 

  • Wellburn FAM, Wellburn AR (1996) Variable patterns of antioxidant protection but similar ethylene differences in several ozone-sensitive and -tolerant selections. Plant Cell Environ 19: 754–760

    Article  CAS  Google Scholar 

  • Wellburn FAM, Creissen GP, Lake J A, Mullineaux PM, Wellburn AR (1998) Tolerance to atmospheric ozone in transgenic tobacco overexpressing glutathione synthetase in plastids. Physiol Plant 104: 623–629

    Article  CAS  Google Scholar 

  • Will B, Eiblmeier M, Langebartels C, Rennenberg H (1997) Consequences, of chronic ozone exposure in transgenic poplars overexpressing enzymes of the glutathione metabolism. In: Cram WJ, De Kok LJ, Stulen I, et al. (eds) Sulphur metabolism in higher plants, molecular, ecophysiological and nutritional aspects. Backhuys, Leiden, Netherlands, pp 257–259

    Google Scholar 

  • Youssefian S, Nakamura M, Sano H (1993) Tobacco plants transformed with the O-acetylserine (thiol) lyase gene of wheat are resistant to toxic levels of hydrogen sulphide gas. Plant J 4: 759–769

    Article  PubMed  CAS  Google Scholar 

  • Youssefian S, Nakamura M, Orudgev E, Kondo N (2001) Increased cysteine biosynthesis capacity of transgenic tobacco overexpressing an O-acetylserine(thiol) lyase modifies plant responses to oxidative stress. Plant Physiol 126 (in press)

    Google Scholar 

  • Yunus M, Singh N, Iqbal M (1996) Global status of air pollution. In: Yunus M, Iqbal M (eds) Plant response to air pollution. J Wiley, London, pp 1–34

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Institute, Faculty of Bioresource Sciences, Akita Prefectural University, Minami 2-2, Ohgata-mura, Akita, 010-0444, Japan

    Shohab Youssefian

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  1. Shohab Youssefian
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Editors and Affiliations

  1. The University of Tokyo, Yahoi 1-1-1, 113-8657, Bunkyo-ku, Tokyo, Japan

    Kenji Omasa Ph.D. (Professor) (Professor)

  2. Molecular Ecotoxicology Section Environmental Biology Division, National Institute for Environmental Studies, Onogawa 16-2, 305-8506, Tsukuba, Ibaraki, Japan

    Hiharu Saji Ph.D. (Head) (Head)

  3. Akita Prefectural University, Minami 2-2, 010-0444, Ohgata-mura, Akita, Japan

    Shohab Youssefian Ph.D. (Associate Professor) (Associate Professor)

  4. The University of Tokyo, Hongo 7-3-1, 113-0033, Bunkyo-ku, Tokyo, Japan

    Noriaki Kondo Ph.D. (Professor) (Professor)

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Youssefian, S. (2002). Manipulation of Genes Involved in Sulfur and Glutathione Metabolism. In: Omasa, K., Saji, H., Youssefian, S., Kondo, N. (eds) Air Pollution and Plant Biotechnology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68388-9_19

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  • DOI: https://doi.org/10.1007/978-4-431-68388-9_19

  • Publisher Name: Springer, Tokyo

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