Field-Portable Imaging System for Measurement of Chlorophyll Fluorescence Quenching
Chlorophyll fluorescence is a powerful tool for noninvasive evaluation of photosynthesis, especially during induction experiments (Govindjee 1995). At ambient temperatures a small amount (about 1%) of photosynthetically active radiation (400–700nm) absorbed by chlorophyll is re-emitted as fluorescence in the near infra-red (>690nm). The fluorescence arises principally from PSII, the primary charge separating and O2 evolving site in photosynthesis, and the quenching of this fluorescence is a reliable indicator of photochemical (useful) and nonphotochemical (wasteful) fates of the absorbed photons (Krause and Weis 1991). The functioning of the multi-component PSII is sensitive to many environmental factors, and as a consequence, fluorescence monitoring has found widespread application in stress physiology and has transformed the assessment of photosynthesis in the field (Osmond et al. 1999).
KeywordsChlorophyll Fluorescence Crown Rust Photosynthetic Induction Leaf Clip Saturate Flash
Unable to display preview. Download preview PDF.
- Chaerle L, Van Der Straeten D (2000) Imaging techniques and early detection of plant stress. TIPS 5: 495–501Google Scholar
- Lichtenthaler HK, Miehe J A (1997) Fluorescence imaging as a diagnostic tool for plant stress. TIPS 2: 316–320Google Scholar
- Lohaus G, Heldt H, Osmond CB (2000) Infection with phloem limited Abutilon mosaic virus causes localised carbohydrate accumulation in leaves of Abutilon striatum: relationships to symptom development and effects on chlorophyll fluorescence quenching during photosynthetic induction. Plant Biol 2: 161–167CrossRefGoogle Scholar
- Mott KA, Buckley TN (2000) patchy stomatal conductance: emergent behaviour of stomata. TIPS 5: 258–262Google Scholar
- Molisch H (1914) Uber die Herstellung von Photographien im Laubblatte. Sitzungsberichte der kaiserlichen Akademie der Wissenschaften, Wein. Reprinted in Molisch, H (1922) Populäre biologische Vorträge. Verlag Gustav Fischer, Jena, pp 243–246Google Scholar
- Omasa K, Murayama S, Matthews MA, Boyer JS (1991) Image diagnosis of photosynthesis in water-deficit plants. In: Hashimoto Y, Day W, Eds., Mathematical and Control Applications in Agriculture and Horticulture. Pergamon Press, Oxford, pp 383–388Google Scholar
- Osmond CB, Berry JA, Balachandran S, Büchen-Osmond C, Daley PF Hodgson RC (1990) Potential consequences of virus infection for shade-sun acclimation in leaves. Bot Acta 103: 226–229Google Scholar
- Osmond CB, Daley PF, Badger MR, Lüttge U (1998) Chlorophyll fluorescence quenching during photosynthetic induction in leaves of Abutilon striatum Dicks, infected with Abutilon mosaic virus, observed with a field-portable imaging system. Bot Acta 111: 390–397Google Scholar
- Schlegel HG (1999) Geschichte der Mikrobiologie. Acta Historica Leopoldina 28: 77–79Google Scholar