Field-Portable Imaging System for Measurement of Chlorophyll Fluorescence Quenching

  • Barry Osmond
  • Yong-Mok Park


Chlorophyll fluorescence is a powerful tool for noninvasive evaluation of photosynthesis, especially during induction experiments (Govindjee 1995). At ambient temperatures a small amount (about 1%) of photosynthetically active radiation (400–700nm) absorbed by chlorophyll is re-emitted as fluorescence in the near infra-red (>690nm). The fluorescence arises principally from PSII, the primary charge separating and O2 evolving site in photosynthesis, and the quenching of this fluorescence is a reliable indicator of photochemical (useful) and nonphotochemical (wasteful) fates of the absorbed photons (Krause and Weis 1991). The functioning of the multi-component PSII is sensitive to many environmental factors, and as a consequence, fluorescence monitoring has found widespread application in stress physiology and has transformed the assessment of photosynthesis in the field (Osmond et al. 1999).


Chlorophyll Fluorescence Crown Rust Photosynthetic Induction Leaf Clip Saturate Flash 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Balachandran S, Osmond CB, Daley PF (1994) Diagnosis of the earliest strain-specific interactions between tobacco mosaic virus and chloroplasts of tobacco leaves in vivo by means of chlorophyll fluorescence imaging. Plant Physiol 104: 1059–1065PubMedGoogle Scholar
  2. Balachandran S, Hurry VM, Kelley SE, Osmond CB, Robinson SA, Rohozinski J, Seaton GGR, Sims DA (1997) Concepts of biotic stress. Some insights into the stress physiology of virus-infected plants, from the perspective of photosynthesis. Physiol Plant 100: 203–213CrossRefGoogle Scholar
  3. Bro E, Meyer S, Genty B (1996) Heterogeneity of leaf C02 assimilation during photosynthetic induction. Plant Cell Environ 19: 1349–1358CrossRefGoogle Scholar
  4. Buschmann C, Lichtenthaler HK (1998) Principles and characteristics of multi-colour fluorescence imaging of plants. J Plant Physiol 152: 297–314CrossRefGoogle Scholar
  5. Chaerle L, Van Der Straeten D (2000) Imaging techniques and early detection of plant stress. TIPS 5: 495–501Google Scholar
  6. Daley PF (1995) Chlorophyll fluorescence analysis and imaging in plant stress and disease. Canad J Plant Pathol 17: 167–173CrossRefGoogle Scholar
  7. Daley PF, Raschke K, Ball, JT, Berry JA (1989) Topography of photosynthetic activity in leaves obtained from video images of chlorophyll fluorescence. Plant Physiol 90: 1233–1238PubMedCrossRefGoogle Scholar
  8. Funayama S, Sonoike K, Terashima I (1997) Photosynthetic properties of Eupatorium makinoi infected by a geminivirus. Photosynth Res 53; 253–261CrossRefGoogle Scholar
  9. Genty B, Meyer S (1995) Quantitative mapping of leaf photosynthesis using chlorophyll fluorescence imaging. Aust J Plant Physiol 22: 277–284CrossRefGoogle Scholar
  10. Govindjee (1995) Sixty three years since Kautsky: chlorophyll a fluorescence. Aust J Plant Physiol 22: 131–160CrossRefGoogle Scholar
  11. Kemp M (2000) Science in culture. Nature 403: 364CrossRefGoogle Scholar
  12. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol. 42: 313–349CrossRefGoogle Scholar
  13. Lichtenthaler HK, Miehe J A (1997) Fluorescence imaging as a diagnostic tool for plant stress. TIPS 2: 316–320Google Scholar
  14. Lohaus G, Heldt H, Osmond CB (2000) Infection with phloem limited Abutilon mosaic virus causes localised carbohydrate accumulation in leaves of Abutilon striatum: relationships to symptom development and effects on chlorophyll fluorescence quenching during photosynthetic induction. Plant Biol 2: 161–167CrossRefGoogle Scholar
  15. Lootens P, Vandercasteele P (2000) A cheap chlorophyll a fluorescence imaging system. Photosynthetica 38: 53–56CrossRefGoogle Scholar
  16. Mott KA, Buckley TN (2000) patchy stomatal conductance: emergent behaviour of stomata. TIPS 5: 258–262Google Scholar
  17. Molisch H (1914) Uber die Herstellung von Photographien im Laubblatte. Sitzungsberichte der kaiserlichen Akademie der Wissenschaften, Wein. Reprinted in Molisch, H (1922) Populäre biologische Vorträge. Verlag Gustav Fischer, Jena, pp 243–246Google Scholar
  18. Ning L, Edwards GE, Strobel A, Daley LS, Callis JB (1995) Imaging fluorometer to detect pathological and physiological change in plants. Appl Spectroscopy 49: 1381–1389CrossRefGoogle Scholar
  19. Omasa K, Shimazaki K-I, Aiga I, Larcher W, Onoe M (1987) Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves. Plant Physiol 84: 748–752PubMedCrossRefGoogle Scholar
  20. Omasa K, Murayama S, Matthews MA, Boyer JS (1991) Image diagnosis of photosynthesis in water-deficit plants. In: Hashimoto Y, Day W, Eds., Mathematical and Control Applications in Agriculture and Horticulture. Pergamon Press, Oxford, pp 383–388Google Scholar
  21. Osmond CB, Berry JA, Balachandran S, Büchen-Osmond C, Daley PF Hodgson RC (1990) Potential consequences of virus infection for shade-sun acclimation in leaves. Bot Acta 103: 226–229Google Scholar
  22. Osmond CB, Daley PF, Badger MR, Lüttge U (1998) Chlorophyll fluorescence quenching during photosynthetic induction in leaves of Abutilon striatum Dicks, infected with Abutilon mosaic virus, observed with a field-portable imaging system. Bot Acta 111: 390–397Google Scholar
  23. Osmond CB, Kramer D, Lüttge U (1999a) Reversible, water stress-induced non-uniform chlorophyll fluorescence quenching in wilting leaves of Potentilla reptans may not be due to patchy stomatal responses. Plant Biol 1: 618–624CrossRefGoogle Scholar
  24. Osmond B, Schwartz O and Gunning B (1999b) Photoinhibitory printing on leaves, visualised by chlorophyll fluorescence imaging and confocal microscopy, is due to diminished fluorescence from grana. Aust J Plant Physiol 26; 717–724CrossRefGoogle Scholar
  25. Peterson RB, Aylor DE (1995) Chlorophyll fluorescence induction in leaves of Phaseolus vulgaris infected with bean rust ( Uromyces appendiculatus ). Plant Physiol 108: 163–171PubMedGoogle Scholar
  26. Rolfe SA, and Scholes JD (1995) Quantitative imaging of chlorophyll fluorescence. New Phytol 131: 69–79CrossRefGoogle Scholar
  27. Schlegel HG (1999) Geschichte der Mikrobiologie. Acta Historica Leopoldina 28: 77–79Google Scholar
  28. Scholes JD, Rolfe SA (1996) Photosynthesis in localised regions of oat leaves infected with crown rust (Puccinia coronata): quantitative imaging of chlorophyll fluorescence. Planta 199: 573–582CrossRefGoogle Scholar
  29. Siebke K, Weis, E (1995a) Assimilation images of leaves of Glechoma hederacea: analysis of non-synchronous stomata related oscillations. Planta 196: 155–165CrossRefGoogle Scholar
  30. Siebke K, Weis E (1995b) Imaging of chlorophyll-a-fluorescence in leaves: topography of photosynthetic oscillations in leaves of Glechoma hederacea. Photosynth Res 45: 225–237CrossRefGoogle Scholar
  31. Tecsi LI, Smith AM, Manie AJ, Leegood RC (1996) A spatial analysis of physiological changes associated with infection of cotyledons of marrow plants with cucumber mosaic virus. Plant Physiol 111: 975–985PubMedGoogle Scholar
  32. Terashima I (1992) Anatomy of non-uniform leaf photosynthesis. Photosynth Res 31: 195–212CrossRefGoogle Scholar

Copyright information

© Springer -Verlag Tokyo 2002

Authors and Affiliations

  • Barry Osmond
    • 1
  • Yong-Mok Park
    • 1
  1. 1.Photobioenergetics Group, Research School of Biological Sciences, Institute of Advanced StudiesAustralian National UniversityWeston CreekAustralia

Personalised recommendations