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Air Pollution and Plant Biotechnology pp 309–319Cite as

Field-Portable Imaging System for Measurement of Chlorophyll Fluorescence Quenching

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Abstract

Chlorophyll fluorescence is a powerful tool for noninvasive evaluation of photosynthesis, especially during induction experiments (Govindjee 1995). At ambient temperatures a small amount (about 1%) of photosynthetically active radiation (400–700nm) absorbed by chlorophyll is re-emitted as fluorescence in the near infra-red (>690nm). The fluorescence arises principally from PSII, the primary charge separating and O2 evolving site in photosynthesis, and the quenching of this fluorescence is a reliable indicator of photochemical (useful) and nonphotochemical (wasteful) fates of the absorbed photons (Krause and Weis 1991). The functioning of the multi-component PSII is sensitive to many environmental factors, and as a consequence, fluorescence monitoring has found widespread application in stress physiology and has transformed the assessment of photosynthesis in the field (Osmond et al. 1999).

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Author information

Author notes

  1. Barry Osmond

    Present address: Biosphere 2 Center, Columbia University, 32540 S Biosphere Road, PO Box 689, Oracle, AZ, 85623, USA

  2. Yong-Mok Park

    Present address: Department of Life Science, College of Natural Science and Engineering, Chongju University, Chongju, 360-764, Korea

Authors and Affiliations

  1. Photobioenergetics Group, Research School of Biological Sciences, Institute of Advanced Studies, Australian National University, Box 3252, Weston Creek, ACT, 2611, Australia

    Barry Osmond & Yong-Mok Park

Authors
  1. Barry Osmond
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  2. Yong-Mok Park
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Editor information

Editors and Affiliations

  1. The University of Tokyo, Yahoi 1-1-1, 113-8657, Bunkyo-ku, Tokyo, Japan

    Kenji Omasa Ph.D. (Professor) (Professor)

  2. Molecular Ecotoxicology Section Environmental Biology Division, National Institute for Environmental Studies, Onogawa 16-2, 305-8506, Tsukuba, Ibaraki, Japan

    Hiharu Saji Ph.D. (Head) (Head)

  3. Akita Prefectural University, Minami 2-2, 010-0444, Ohgata-mura, Akita, Japan

    Shohab Youssefian Ph.D. (Associate Professor) (Associate Professor)

  4. The University of Tokyo, Hongo 7-3-1, 113-0033, Bunkyo-ku, Tokyo, Japan

    Noriaki Kondo Ph.D. (Professor) (Professor)

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© 2002 Springer -Verlag Tokyo

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Osmond, B., Park, YM. (2002). Field-Portable Imaging System for Measurement of Chlorophyll Fluorescence Quenching. In: Omasa, K., Saji, H., Youssefian, S., Kondo, N. (eds) Air Pollution and Plant Biotechnology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68388-9_16

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  • DOI: https://doi.org/10.1007/978-4-431-68388-9_16

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68390-2

  • Online ISBN: 978-4-431-68388-9

  • eBook Packages: Springer Book Archive

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