Summary
With the aid of a novel blood filtration system into which microbeads coated with confluent endothelial cells can be incorporated, cellular interactions can now be investigated in streaming whole blood in vitro under conditions largely approximating those in vivo. Reductions in filtration rate are elicited by f-MLP, ADP, and PAF in a concentration-dependent manner, due to the activation and aggregation of neutrophils (PMN) and platelets. These effects can be antagonized by exogenously applied adenosine, PGE1 and PGE2 in physiological concentrations. Cultured coronary endothelial cells also inhibit the actions of the above-listed mediators of inflammation on these blood cells, presumably by releasing the corresponding endogenously produced adenosine and prostaglandins into the streaming blood. In the physiological state, this inhibitory effect of the endothelium probably suppresses the manifestation of inflammatory processes in the intact parts of the coronary system downstream of the inflammatory site.
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© 1991 Springer Japan
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Nees, S., Dendorfer, A. (1991). Inhibition of PMN and Platelets in the Coronary System by Endothelium-Derived Adenosine, PGE1 and PGE2 . In: Inoue, M., Hori, M., Imai, S., Berne, R.M. (eds) Regulation of Coronary Blood Flow. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68367-4_14
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DOI: https://doi.org/10.1007/978-4-431-68367-4_14
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