Assessment of Malignancy of Bone and Soft Tissue Tumors Using DNA Cytofluorometry

  • Katsuyuki Kusuzaki
  • Tsukasa Ashihara
  • Yasusuke Hirasawa
Conference paper


Many recent reports have demonstrated the usefulness of cytometric DNA ploidy analysis in assessing the nature of the malignancy of human tumors. Among the researchers who have attempted over the past 10 years to apply this technique to bone and soft tissue tumors [1–7], most believe that DNA ploidy analysis may be helpful not only for the histological diagnosis of malignancy but also in predicting prognosis. Most of these previous studies used flow cytometry, a method in which examiners are unable to determine the morphology of the studied cells, even though a large number of cells can be measured instantly. However, flow cytometry may not provide an accurate measurement of DNA content due to possible contamination with non-tumorous cells, such as fibroblasts, granulocytes, and lymphocytes, and a large amount of debris from bony, cartilaginous, and collagenous matrices that commonly appear in bone and soft tissue tumors. In order to avoid these problems, we attempted to analyze the DNA ploidy of bone and soft tissue tumors using cytofluorometry. With this method, we were able to selectively measure only the tumor cells under a fluorescence microscope, although the measurable cell number was much lower than that obtained with flow cytometry and it was relatively time-consuming because we had to detect cells which had been smeared on a glass slide. The results of DNA ploidy analysis by cytofluorometry were statistically comparable with histological evaluation of malignancy performed by pathologists.


Bone Tumor Synovial Sarcoma Soft Tissue Tumor Giant Cell Tumor Giant Cell Tumor 
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Copyright information

© Springer Japan 1992

Authors and Affiliations

  • Katsuyuki Kusuzaki
    • 1
  • Tsukasa Ashihara
    • 2
  • Yasusuke Hirasawa
    • 1
  1. 1.Department of Orthopaedic SurgeryKyoto Prefectural University of MedicineKamigyo-ku, Kyoto, 602Japan
  2. 2.Department of PathologyKyoto Prefectural University of MedicineKamigyo-ku, Kyoto, 602Japan

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