Assessment of Malignancy of Bone and Soft Tissue Tumors Using DNA Cytofluorometry
Many recent reports have demonstrated the usefulness of cytometric DNA ploidy analysis in assessing the nature of the malignancy of human tumors. Among the researchers who have attempted over the past 10 years to apply this technique to bone and soft tissue tumors [1–7], most believe that DNA ploidy analysis may be helpful not only for the histological diagnosis of malignancy but also in predicting prognosis. Most of these previous studies used flow cytometry, a method in which examiners are unable to determine the morphology of the studied cells, even though a large number of cells can be measured instantly. However, flow cytometry may not provide an accurate measurement of DNA content due to possible contamination with non-tumorous cells, such as fibroblasts, granulocytes, and lymphocytes, and a large amount of debris from bony, cartilaginous, and collagenous matrices that commonly appear in bone and soft tissue tumors. In order to avoid these problems, we attempted to analyze the DNA ploidy of bone and soft tissue tumors using cytofluorometry. With this method, we were able to selectively measure only the tumor cells under a fluorescence microscope, although the measurable cell number was much lower than that obtained with flow cytometry and it was relatively time-consuming because we had to detect cells which had been smeared on a glass slide. The results of DNA ploidy analysis by cytofluorometry were statistically comparable with histological evaluation of malignancy performed by pathologists.
KeywordsBone Tumor Synovial Sarcoma Soft Tissue Tumor Giant Cell Tumor Giant Cell Tumor
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- 2.Mankin HJ, Connor JF, Schiller AL, Perlmutter A, Alho A, McGuire M (1985) Grading of bone tumors by analysis of nuclear DNA content using flow cytometry. J Bone Joint Surg [Am] 67: 404–413Google Scholar
- 7.Matsuno T, Gebhardt MC, Schiller AL, Rosenberg AE, Mankin Hi (1988) The use of flow cytometry as a diagnostic aid in the management of soft tissue tumors. J Bone Joint Surg [Am] 70: 751–759Google Scholar
- 9.Kusuzaki K, Takeshita H, Kuzuhara A, Bann S, Yamashita F, Sakakida K, Kamachi M, Tsuchihashi Y, Ashihara T (1984) DNA cytofluorometry of soft tissue tumors. Jpn J Cancer Clin 30: 1904–1912Google Scholar
- 10.Kusuzaki K, Takashita H, Bann S, Kamachi M, Yamashita F, Ashihara T, Sakakida K (1985) An attempt of DNA cytofluorometry of bone tumors (abstract). Seikeigeka 36: 647–653Google Scholar
- 11.Takeshita H, Kusuzaki K, Kuzuhara A, Bann S, Yamashita F, Sakakida K, Kamachi M, Ashihara T (1985) DNA cytofluorometric analysis of nerve sheath tumors. J Jpn Orthop Assoc 59: 763–772Google Scholar
- 12.Kusuzaki K, Takeshita H, Kuzuhara A, Bann S, Yamashita F, Sakakida K, Kamachi M, Ashihara T (1986) The relationship between cell kinetics and histological features of giant cell tumor of bone. J Jpn Orthop Assoc 60: 51–60Google Scholar
- 13.Kusuzaki K, Takeshita H, Kuzuhara A, Tsuji Y, Yamashita F, Sakakida K, Kamachi M, Ashihara T (1986) Biological malignancy of giant cell tumors of bone as analyzed by cell proliferative activity using DNA cytofluorometry (abstract). Seikeigeka: 38: 559–567Google Scholar