Metabolism of Inositol Polyphosphates by Malignant Hyperthermia-Susceptible and Control Porcine Skeletal Muscle
Malignant hyperthermia-susceptible (MH-susceptible, MHS) skeletal muscle cells have an inherited abnormality in the processes that are intimately involved in excitation-contraction coupling (EC coupling) or myoplasmic Ca2+ regulation [1,2]. MHsusceptible muscle cells have an elevated level of myoplasmic Ca2+, and a rapid and sustained rise in myoplasmic Ca2+ is also central to the etiology of fulminant anesthetic-induced MH. The mechanism of signal transduction across the triadic junction during EC coupling of skeletal muscle is unknown . However, mechanical coupling between highly specialized triadic proteins has been proposed as the primary mechanism for voltage-activated generation of sarcoplasmic reticulum (SR) Ca2+ signals and subsequent construction. The dihydropyridine-sensitive Ca2+ channel (voltage sensor) of the transverse tubule (T-tubule) membrane and the ryanodine receptor or Ca2+ release channel of the SR are key proteins involved in this process .
KeywordsHigh Pressure Liquid Chromatography Inositol Phosphate Malignant Hyperthermia Soluble Extract Inositol Polyphosphate
Unable to display preview. Download preview PDF.
- 6.Otsu K, Nishida K, Kimura Y, Kuzuya T, Hori M, Kamada T, Tada M (1994) The point mutation Arg615–Cys in the Ca2+ release channel of skeletal sarcoplasmic reticulum is responsible for hypersensitivity to caffeine and halothane in malignant hyperthermia. J Biol Chem 265: 13472–13483Google Scholar
- 7.Quane KA, Keating KE, Manning BM, Healy JMS, Monsieurs K, Heffron JJA, Lehane M, Heytens L, Krisovic-Horber R, Adnet P, et al (1994) Detection of a novel common mutation in the ryanodine receptor gene in malignant hyperthermia: implications for diagnosis of heterogeneity studies. Hum Mol Genet 3: 471–476PubMedCrossRefGoogle Scholar
- 10.Lopez JR, Perez C, Alfonzo M, Cordovez G, Linares N, Allen PD (1993) Changes in intracellular Ca2+ concenration induced by inositol 1,4,5-trisphoshate in human malignant hyperthermia skeletal muscle. Biophys (Life Sci Adv) 12: 131–140Google Scholar