The DiFi Rectal Carcinoma Cell Line from a Familial Adenomatous Polyposis Patient: An In Vitro Model System to Study Genetic Changes in Colorectal Cancer
A new human colorectal cancer cell line (DiFi) has been established from a patient with the Gardner Syndrome. Characterization studies have shown that DiFi cells secrete carcinoembryonic antigen (CEA) and CA 19-9, are tumorigenic in nude mice, have a tetraploid karyotype, and possess an isozyme pattern characteristic of colorectal cancer cell lines. Southern blot analysis shows that DiFi cells have allelic loss on chromosomes 17p and 18, plus amplification of the EGF receptor gene. Northern blot analysis reveals that transforming growth factor (TGF)-alpha, c-myc, and p53 transcripts are clearly expressed in these cells. The significance of these molecular changes in relation to mechanisms leading to a selective growth advantage in DiFi cells will be discussed.
KeywordsFamilial Adenomatous Polyposis Lynch Syndrome Colorectal Cancer Cell Line Familial Adenomatous Polyposis Patient Transform Growth Factor Alpha
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