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The DiFi Rectal Carcinoma Cell Line from a Familial Adenomatous Polyposis Patient: An In Vitro Model System to Study Genetic Changes in Colorectal Cancer

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Hereditary Colorectal Cancer

Abstract

A new human colorectal cancer cell line (DiFi) has been established from a patient with the Gardner Syndrome. Characterization studies have shown that DiFi cells secrete carcinoembryonic antigen (CEA) and CA 19-9, are tumorigenic in nude mice, have a tetraploid karyotype, and possess an isozyme pattern characteristic of colorectal cancer cell lines. Southern blot analysis shows that DiFi cells have allelic loss on chromosomes 17p and 18, plus amplification of the EGF receptor gene. Northern blot analysis reveals that transforming growth factor (TGF)-alpha, c-myc, and p53 transcripts are clearly expressed in these cells. The significance of these molecular changes in relation to mechanisms leading to a selective growth advantage in DiFi cells will be discussed.

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Authors
  1. Bruce M. Boman
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  2. Matilde Olive
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  3. Seema Untawale
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  4. Mark Blick
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  5. Susan North
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  6. Gary Gallick
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  7. Gaudenz Dolf
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  8. Michael J. Siciliano
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  9. Leor D. Roubein
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  10. Herbert Fritche
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  11. Gilles Thomas
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  12. Sen Pathak
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  13. David M. Wildrick
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  14. Bernard Levin
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Editor information

Editors and Affiliations

  1. Second Department of Surgery, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663, Japan

    Joji Utsunomiya M. D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Preventive Medicine, Creighton University School of Medicine, Hereditary Cancer Center, California at 24th Street, Omaha, NE, 68178, USA

    Henry T. Lynch M. D. (Professor and Chairman) (Professor and Chairman)

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© 1990 Springer Japan

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Boman, B.M. et al. (1990). The DiFi Rectal Carcinoma Cell Line from a Familial Adenomatous Polyposis Patient: An In Vitro Model System to Study Genetic Changes in Colorectal Cancer. In: Utsunomiya, J., Lynch, H.T. (eds) Hereditary Colorectal Cancer. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68337-7_80

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  • DOI: https://doi.org/10.1007/978-4-431-68337-7_80

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68339-1

  • Online ISBN: 978-4-431-68337-7

  • eBook Packages: Springer Book Archive

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