Ex Vivo Expansion of Human Primitive Hemopoietic Progenitors
The hemopoietic system is characterized by continuous cell turnover supported by a small populations of cells termed hemopoietic stem cells. It is generally held that multipotential hemopoietic stem cells possess extensive capabilities to self-renew and generate various committed progenitor cells(1). Intercellular communication in the hemopoietic system is mediated by soluble factors called cytokines, whose functional characteristics are now partly explained by their receptor system (2–5). Most cytokine receptor systems consist of a multi-chain complex, a ligand-specific receptor chain (α-chain) and a signal transducing chain (β-chain), the latter of which is often utilized in common by several receptor complexes(2,6).
KeywordsHuman CD34 Hemopoietic Stem Cell Hemopoietic Progenitor Hemopoietic System Signal Transduce Chain
Unable to display preview. Download preview PDF.
- 8.Saito, M., Yoshida, K., Hibi, M., Taga, T. & Kishimoto, T.: Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo. J. Immuno. 148, 4066, 1992.Google Scholar
- 9.Koike, K., T. Nakahata, T. Takagi, T. Kobayashi, A. Ishiguro, K. Tsuji, K. Naganuma, A. Okano, Y. Akiyama, and T. Akabane.: Synergism of BSF-2/interlukin 6 and interlukin 3 on development of multi-potential hemopoietic progeniotrs in serum-free culture. J. Exp. Med. 168: 879, 1988.PubMedCrossRefGoogle Scholar
- 11.Sui X, Tsuji K, Tajima S, Tanaka R, Muraoka K, Ebihara Y, Ikebuchi K, Yasukawa K, Taga T, Kishimoto T, Nakahata T Erythropoietinindependent erythrocyte production: signals through gp130 and c-Kit dramatically promote erythropoiesis from human CD34+ cells. J. Exp. Med. in press.Google Scholar
- 13.Yoshida K, Taga T, Saito M, Suematsu S, Kumanogoh A, Tanaka T, Fujiwara H, Hirata M, Yamagami T, Nakahata T, Hirabayashi T, Yoneda Y, Tanaka K, Wang WZ, Mori C, Shiota K, Yoshida N, and Kishimoto T Targeted disruption of gp130, a common signal transducer for IL-6-family of cytokines, leads to myocardial and hematological disorders. Proc. Natl. Acad. Sci. USA in press.Google Scholar
- 16.Lanley KE, Bennett LG, Wypych J, Yancik SA, Liu XD, Westcott KR, Chang WD, Smith KA, Zsebo KM.: Soluble stem cell factor in human serum. Blood 81: 656, 1993.Google Scholar
- 17.Montero-Julian FA, Liautard J, Flavetta S, Romagne F, Gaillard JP, Brochier J, Klein B, Brailly H.: Immunoassay for functional human soluble interleukin-6 receptor in plasma based on ligand/receptor interactions. J. Immuno. Methods 169: 111, 1994.Google Scholar