Summary
HTK is a receptor tyrosine kinase (RTK) which belongs to the Eph subfamily of RTK. Using a BlAcore, a surface plasmon resonance detection system, it was determined that a colon cancer cell line C-1 expressed HTK ligand (HTKL). From the conditioned medium of C-1 cells, a soluble form of HTKL was purified by receptor affinity chromatography. The N-terminal amino acid sequence of purified HTKL was determined and the full-length cDNA of HTKL was isolated from a C-1 cell cDNA library. HTKL is a type I transmembrane protein which consists of 333 amino acids. HTK receptor tyrosine phosphorylation was induced by membrane-bound or clustered soluble ligands but not by unclustered soluble ligands, indicating that HTKL requires cell-to-cell interaction for receptor phosphorylation. FACS analysis of human bone marrow mononuclear cells showed that HTK receptor was expressed in CD34lowc-KIT+ hematopoietic progenitor cell fraction. These findings suggest the interaction of hematopoietic progenitor cells with HTKL-expressing cells in bone marrow and the involvement of HTKL in the differentiation and/or proliferation of these progenitor cells.
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© 1996 Springer-Verlag Tokyo
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Sakano, S. et al. (1996). Identification and Characterization of a Ligand for Receptor Protein-Tyrosine Kinase HTK Expressed in Hematopoietic Cells. In: Ikehara, S., Takaku, F., Good, R.A. (eds) Bone Marrow Transplantation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68320-9_5
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DOI: https://doi.org/10.1007/978-4-431-68320-9_5
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