Summary
We developed an efficient differentiation induction system from mouse embryonic stem (ES) cells into blood cells by coculture on a novel stromal cell line named OP9. ES cells could give rise to adult type definitive erythrocytes, myeloid and B lineage cells, when the cells were simply cocultured with the OP9 stromal cells. This stromal cell line does not produce functional macrophage colony-stimulating factor (M-CSF) and presumably the deficiency of M-CSF production of OP9 cells might induce preferential differentiation into hematopoietic cells other than monocyte-macrophage lineage. Unfortunately, there did not appear to be any self-renewing hematopoietic stem cells to produce spleen colonies or bring about long term reconstitution of hematopoiesis in lethally irradiated mice during the differentiation induction. Several systems of in vitro differentiation induction from ES cells to lympho-hematopoietic cells are summarized. The usefulness and the limitation of the in vitro differentiation induction systems are discussed.
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© 1996 Springer-Verlag Tokyo
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Nakano, T., Era, T., Kodama, H., Honjo, T. (1996). Development of Blood Cells from Mouse Embryonic Stem Cells in Culture. In: Ikehara, S., Takaku, F., Good, R.A. (eds) Bone Marrow Transplantation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68320-9_2
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DOI: https://doi.org/10.1007/978-4-431-68320-9_2
Publisher Name: Springer, Tokyo
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