Sequential Changes of Brain Metabolism During Temporary Global Ischemia in the Rat Detected by Simultaneous 1H and 31P MRS

  • Reizo Shirane
  • Lee-Hong Chang
  • Philip R. Weinstein
  • Thomas L. James


In vivo magnetic resonance spectroscopy (MRS) provides a noninvasive method for evaluating brain metabolism. Previous studies with 31P MRS have developed techniques for continuous monitoring of phosphate metabolites and intracellular pH (pHi) during ischemia and reperfusion [1]. Furthermore, recent progress has made it possible to monitor brain lactate content quantitatively with 1H MRS [2]. In this study, we utilized a double-tuned surface coil for 1H and 31P MRS. With this recently described method, in vivo 1H and 31P spectroscopy can be carried out simultaneously without retuning or replacement of the coil [3].


Nuclear Magnetic Resonance Spectroscopy Brain Energy Metabolism Complete Ischemia Incomplete Ischemia Acetyl Aspartate 
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  1. 1.
    Andrews BT, Keniry MA, Richards TR, et al. (1987) 31-phosphorus nuclear magnetic resonance spectroscopy in global cerebral ischemia and reperfusion in the rat. Neurosurgery 5:699–708CrossRefGoogle Scholar
  2. 2.
    Chang LH, Pereira BM, Keniry MA, et al. (1987) Comparison of lactate concentration determinations in ischemic and hypoxic rat brains by in vivo and in vitro 1H NMR spectroscopy. Magn Reson Med 4:575–581PubMedCrossRefGoogle Scholar
  3. 3.
    Chang LH, Chew WM, Weinstein PR, et al. (1987) A balanced-matched double-tuned probe for in vivo 1H and 31P NMR. J Magn Reson 72:168–172Google Scholar
  4. 4.
    Gonzalez-Mendez R, Litt L, Koretsky AP, et al. (1984) Comparison of 31P NMR spectra of in vivo rat brain using convolution difference and saturation with a surface coil: Source of the broad component in the brain spectrum. J Magn Reson 57:526–533Google Scholar
  5. 5.
    von Hanwehr R, Smith ML, Siesjo BK (1986) Extra-and intracellular pH during near-complete forebrain ischemia in the rat. J Neurochem 46:331–339CrossRefGoogle Scholar
  6. 6.
    Kameyama M, Suzuki J, Shirane R, et al. (1985) A new model of bilateral hemispheric ischemia in the rat: Three-vessel occlusion model. Stroke 16:489–493PubMedCrossRefGoogle Scholar
  7. 7.
    Ljunggren B, Norberg K, Siesjo BK (1974) Influence of tissue acidosis upon restitution of brain energy metabolism following total ischemia. Brain Res 77:173–186PubMedCrossRefGoogle Scholar
  8. 8.
    Petroff OAC, Prichard JW, Behar KL, et al. (1985) Cerebral intracellular pH by 31P nuclear magnetic resonance spectroscopy. Neurology 35:781–788PubMedCrossRefGoogle Scholar
  9. 9.
    Seo Y, Murakami M, Watari H, et al. (1983) Intracellular pH determination by a 31P NMR technique: The second dissociation constant of phosphoric acid in a biological system. J Biochem 94:729–734PubMedGoogle Scholar
  10. 10.
    Yoshida S, Busto R, Martinez, et al. (1985) Regional brain energy metabolism after complete versus incomplete ischemia in the rat in the absence of severe lactic acidosis. J Cereb Blood Flow Metabol 5:490–501CrossRefGoogle Scholar

Copyright information

© Springer Japan 1988

Authors and Affiliations

  • Reizo Shirane
    • 1
  • Lee-Hong Chang
    • 1
    • 2
  • Philip R. Weinstein
    • 1
  • Thomas L. James
    • 2
  1. 1.Department of NeurosurgeryUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of Pharmaceutical ChemistryUniversity of CaliforniaSan FranciscoUSA

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