The Effect of Mannitol, Nimodipine, and Indomethacin, Singly or in Combination, on Cerebral Ischemia

  • Garnette Sutherland
  • Howard Lesiuk
  • Ranjan Bose
  • Anders A. F. Sima
Conference paper


The effects of mannitol, nimodipine, and indomethacin on ischemic neuronal injury were examined in 45 rats (divided equally into nine groups) subjected to 10 min of forebrain ischemia. Of two control groups, one received maintenance fluids, while the other, a normal saline bolus. In the remaining seven groups, mannitol, nimodipine, and indomethacin were administered either singly or in combination 5 min prior to forebrain ischemia. Seven days postischemia, the brains were perfusion-fixed, sectioned coronally into 2.8-mm slices, and stained with hematoxylin and eosin. Quantification of dying neurons was performed by direct counting of standardized levels chosen from the serial sections.


Ischemic Injury Neuronal Injury Ischemic Insult Cereb Blood Flow Forebrain Ischemia 
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  1. 1.
    Ames A, Nesbett FB (1983) Pathophysiology of ischemic cell death. 1. Time of onset of irreversible damage; importance of the different components of the ischemic insult. Stroke 14:219–226PubMedCrossRefGoogle Scholar
  2. 2.
    Cheung JY, Bonventre JV, Malis CD, Leaf A (1986) Calcium and ischemic injury. N Engl J Med 314:1670–1676PubMedCrossRefGoogle Scholar
  3. 3.
    Dienel GA, Pulsinelli WA, Duffy TE (1980) Regional protein synthesis in rat brain following acute hemispheric ischemia. J Neurochem 35:1216–1226PubMedCrossRefGoogle Scholar
  4. 4.
    Harris R, Symon L (1984) Extracellular pH, potassium and calcium activities in progressive ischaemia of rat cortex. J Cereb Blood Flow Metabol 4:178–186CrossRefGoogle Scholar
  5. 5.
    Little JR (1979) Treatment of acute focal cerebral ischemia with intermittent boluses of low-dose mannitol. Neurosurgery 5(6):687–691PubMedCrossRefGoogle Scholar
  6. 6.
    Shigeno S, Fritschka E, Shigeno T, Brock M (1985) Effects of indomethacin on rCBF during and after focal cerebral ischemia in the cat. Stroke 16(2):235–240PubMedCrossRefGoogle Scholar
  7. 7.
    Siesjo BK (1981) Cell damage in the brain: A speculative synthesis. J Cereb Blood Flow Metabol 1:155–185CrossRefGoogle Scholar
  8. 8.
    Smith M-L, Auer RN, Siesjo BK (1984) The density and distribution of ischemic brain injury in the rat following 2–10 min of forebrain ischemia. Acta Neuropathol (Berl.) 64:319–332CrossRefGoogle Scholar
  9. 9.
    Steen PA, Newberg La, Milde JH, et al. (1983) Nimodipine improves cerebral blood flow and neurologic recovery after complete cerebral ischemia in the dog. J Cereb Blood Flow Metab 3:38PubMedCrossRefGoogle Scholar
  10. 10.
    Symon L (1985) Flow thresholds in brain ischemia and the effects of drugs. Br J Anaesth 57:34–43PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 1988

Authors and Affiliations

  • Garnette Sutherland
    • 1
    • 2
  • Howard Lesiuk
    • 1
    • 2
  • Ranjan Bose
    • 1
  • Anders A. F. Sima
    • 3
  1. 1.Departments of PharmacologyThe University of ManitobaWinnipegCanada
  2. 2.Departments of SurgeryThe University of ManitobaWinnipegCanada
  3. 3.Departments of PathologyThe University of ManitobaWinnipegCanada

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