The Effect of Mannitol, Nimodipine, and Indomethacin, Singly or in Combination, on Cerebral Ischemia
The effects of mannitol, nimodipine, and indomethacin on ischemic neuronal injury were examined in 45 rats (divided equally into nine groups) subjected to 10 min of forebrain ischemia. Of two control groups, one received maintenance fluids, while the other, a normal saline bolus. In the remaining seven groups, mannitol, nimodipine, and indomethacin were administered either singly or in combination 5 min prior to forebrain ischemia. Seven days postischemia, the brains were perfusion-fixed, sectioned coronally into 2.8-mm slices, and stained with hematoxylin and eosin. Quantification of dying neurons was performed by direct counting of standardized levels chosen from the serial sections.
KeywordsIschemic Injury Neuronal Injury Ischemic Insult Cereb Blood Flow Forebrain Ischemia
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