Antigenicity of the Cuticle with Emphasis on the Immunocytochemical Approach

  • Y. Takahashi
  • T. Araki


Since the introduction of immunostaining technology, our knowledge of reading and interpretating histological specimens has expanded enormously. This powerful tool allows us to study the in situ localization of the antigen providing new insights into both experimental and clinical immuno-parasitology. However, inherent problems such as false negative and false positive results are always present in spite of technological advancements in resin, antibody and stain enhancement. In this article, we briefly explain current immunostaining techniques, with emphasis on why immunostaining of the cuticle inner-layers is so difficult.


Visual Marker Ascaris Lumbricoides Muscle Larva Cuticle Surface Cuticular Surface 
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  1. 1.
    Beesley JF (1985) Colloidal gold probes for parasite antigens. Parasitol Today 1: 145–146PubMedCrossRefGoogle Scholar
  2. 2.
    Brzosko WJ, Gancarz Z (1969) Immunoelectronoscopic studies on antibody binding to the cuticle of Trichinella spiralis larvae. Wiadomosci Parazytologiczne T. XV, Nr 5–6Google Scholar
  3. 3.
    Chen SN, Howells RE (1979) The uptake in vitro of dyes, monosaccharides and aminoacids by the filarial worm Brugia pahangi. Parasitology 78: 343–354PubMedCrossRefGoogle Scholar
  4. 4.
    Crandall CA, Echevarria R, Arean VM (1963) Localization of antibody binding sites in the larvae of Ascaris lumbricoides var. suum by means of fluorescent techniques. Exp Parasitol 14: 296–303PubMedCrossRefGoogle Scholar
  5. 5.
    Despommier DD (1974) The stichocyte of Trichinella spiralis during morphogenesis in the small intestine of the rat. In: Kim C (ed) Trichinellosis, Intext Educational Publishers, New York, pp. 239–254Google Scholar
  6. 6.
    Despommier DD, Kajima M, Wostman S (1967) Ferritin-conjugated antibody studies on the larva of Trichinella spiralis. J Parasitol 53: 618–624PubMedCrossRefGoogle Scholar
  7. 7.
    Gadea DL, Moore LA, Oliver-Gonzalez J (1967) Adsorption of globulin to the cuticle oflarvae and adults of Trichinella spiralis. Am J Trap Med Hyg 16(6): 750–751Google Scholar
  8. 8.
    Jungery M, Clark NWT, Parkhouse RM (1983a) A major change in surface antigens during the maturation of newborn larvae of Trichinella spiralis. Mol Biochem Parasitol 7: 101–109PubMedCrossRefGoogle Scholar
  9. 9.
    Jungery M, Mackenzie CD, Ogilvie BM (1983b) Some properties of the surface of nematode larvae. J Helminthol 57: 291–295PubMedCrossRefGoogle Scholar
  10. 10.
    Kim CW, Ledbetter MC (1981) Detection of specific antigen-antibody precipitates on the surface of Trichinella spiralis by scanning electron microscopy. In: Kim CW, Ruitenberg EJ, Teppema JS (eds) Trichinellosis, Reedbooks, Chertsey, England, pp.65–69Google Scholar
  11. 11.
    Leventhal R, Soulsby EJL (1977) Ascalis suum: Cuticular binding of the third component of complement by early larval stages. Exp Parasitology 41: 423–431CrossRefGoogle Scholar
  12. 12.
    Maize1s RM, Philipp M, Ogilvie BM (1982) Molecules on the surface of parasitic nematodes as probes of the immune response in infection. Immunological Rev 61: 109–136CrossRefGoogle Scholar
  13. 13.
    McLaren DJ, Mackenzie CD, Ramaho-Pinto FJ (1977) Ultrastructural observations on the in vitro interaction between rat eosinophils and some parasitic helminths. Clin Exp Immunology 30: 105–118Google Scholar
  14. 14.
    Murrell KD, Graham C (1982) Solubilization studies on the epicuticular antigens of Strongyloides ratti. Veterinary Parasitology 10: 191–203PubMedCrossRefGoogle Scholar
  15. 15.
    Ogilvie BM, Philipp M, Jungery M, Maizeles RM, Worms MJ, Parkhouse RME (1980) The surface of nematodes and the immune response of the host. In: Van den Bossche H (ed) The host Invader Interplay, Elsevier, Amsterdam, pp. 99–104Google Scholar
  16. 16.
    Ogimoto K (1984) Study on immunofluorescence of Japanese strain of Trichinella spiralis (Iwasaki strain, Yamagichi 1974) V. Immunoelectron and immunofluorescent microscopic localization of antigen. Hirosaki Med J 36: 283–296Google Scholar
  17. 17.
    Parkhouse RME, Phillip M, Ogilvie BM (1981) Characterization of surface antigens of Trichinella spiralis infectivae larvae. Parasit Immunol 3: 339–352CrossRefGoogle Scholar
  18. 18.
    Perrudet-Badoux A, Anteunis A, Dunitresucu SM, Binaghi RA (1978) Ultrastructural study of the immune interaction between perioneal cells of larvae of Trichinella spiralis. J Reticuloendothel Soc 24 (3): 311–314PubMedGoogle Scholar
  19. 19.
    Philipp M, Parkhouse RME, Ogilvie BM (1980) Changing proteins on the surface of a parasitic nematode. Nature (London) 287: 538–540CrossRefGoogle Scholar
  20. 20.
    Philipp M, Parkhouse RME, Ogilvie BM (1981) The molecular basis for stage specificity of the primary antibody response to the suface of Trichinella spiralis. In: Kim CW, Ruitenberg FJ, Teppema JS (eds), Trichinellosis, Reedbooks, Chertsey, England, pp. 59–64Google Scholar
  21. 21.
    Philipp M, Worms MJ, McLaren DJ, Ogilvie BM, Parkhouse RM, Taylor PM (1984) Surface proteins of a filarial nematode: a major soluble antigen and a host component on the cuticle of Litomosoides carinii.. Parasit Immunol 6: 63–82CrossRefGoogle Scholar
  22. 22.
    Pritchard DI, Crawford CR, Duce IR, Behnke JM (1985) Antigen stripping from a nematode epicuticle using the cationic detergent cetyl trim ethyl ammonium bromide (CTAB). Parasit Immunol 7: 575–585CrossRefGoogle Scholar
  23. 23.
    Stankiewicz M, Jeska EL (1973) Leukocytes and Trichinella spiralis. Immunology 25: 827–834PubMedGoogle Scholar
  24. 24.
    Takahashi Y, Uno T, Nishiyama T, Yamada S, Araki T (1988) Immunocytolocalization study of the external covering of Trinchinella spiralis muscle larva. J Parasitol 74: 270–274PubMedCrossRefGoogle Scholar
  25. 25.
    Tam ND, Paradimitriou J, Keast D (1983) The location of antigenic sites on the surface of the nematode parasite Strongyloides ratii reactive to antibody-directed cellular cytotoxicity from both macrophages and eosinophils from the natural host. Aust J Exp Boil Med Sci 61: 629–636CrossRefGoogle Scholar
  26. 26.
    Vernes A, Poulain D, Prensier G, Deblock S, Biguet J, Wattez A (1974) Trichinose experimentale. III. Action in vitro des cellules peritoneales sensibilsees sur les larves musculaires des premiers stades: Etude preliminaire comparative en microscopie optique et electronique a transmission et balayage. Biomedicine 21: 140–145PubMedGoogle Scholar

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© Springer-Verlag Tokyo 1990

Authors and Affiliations

  • Y. Takahashi
  • T. Araki

There are no affiliations available

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