Detection of Cellular Immunity by Migration Inhibition Test on Rabbits and Guinea Pigs Immunized with Anisakis Larval Antigens

  • Y. Kikuchi
  • H. Saeki
  • H. Ishikura


It is well documented that cell-mediated immunity (CMI) plays an important role in the protection of the host against parasite infections. Some earlier workers detected the development of CMI from several Protozoa [1–3], Schistosoma [4–6] or Trichina worm [7], Echinococcus infections [8–10] and others [11,12] by the appearance of delayed type hypersensitivity skin reactions and/or CMI in vitro reactions.


Macrophage Migration Inhibition Factor Migration Inhibition Migration Index Alveolar Echinococcosis Macrophage Migration Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Haq A, Sharma A, Ahamad S (1984) Increased macrophage migration inhibition factor production in hamsters sensitized by amoebic antigen and glycan. Parasitic Immunology 6: 391–396CrossRefGoogle Scholar
  2. 2.
    De Rossell RA, Bray RS, Alexander J (1987) The correlation between delayed hypersensitivity, lymphocyte activation and protective immunity in experimental murine leishmaniasis. Parasite Immunology 9: 105–115PubMedCrossRefGoogle Scholar
  3. 3.
    Fahey JR, Herman R (1981) Relationship between delayed hypersensitivity response and acquired cell-mediated immunity in C57BL/6J mice infected with Leishmania donovani. Infection and Immunity 49: 447–451Google Scholar
  4. 4.
    Stephen W, Boros DL, David CS (1983) Regulation of granulomatous inflammation in murine schistosomiasis. 11. T suppresser cell-derived, I-C subregion-encoded soluble suppressor factor mediates regulation of Iymphokine production. J Exp Med 157: 219–230CrossRefGoogle Scholar
  5. 5.
    Auriault C, Balloul J, Pierce RJ, Damonneville M, Sondermeijer P, Capron A (1987) Helper T cells induced by a purified 28-kilodalton antigen of Schistosoma mansoni protect rats against infection. Infection and Immunity 55: 1163–1169PubMedGoogle Scholar
  6. 6.
    Lewis FA, Winestock J, James SL (1987) Macrophage activation as an immune correlate to protective immunity against Schistosomiasis in mice immunized with an irradiated, cryopreserved live vaccine. Infection and Immunity, 55: 1339–1345PubMedGoogle Scholar
  7. 7.
    Palmas C, Conchedda M, Bortoletti G, Gabriele H (1985) In vitro cellular immune response in mice and rabbits immunized with Trichinella spiralis antigen. Parassitologia 64: 216–222Google Scholar
  8. 8.
    Phillips SM, Fox EG (1984) Immunopathology of parasitic disease: a conceptual approach. Contemp Top Immunobiol 12: 421–461PubMedGoogle Scholar
  9. 9.
    Vuitton DA, Lassegue A, Miguet JP, Herve P, Barale T, Seilles E, Capron A (1984) Humoral and cellular immunity in patients with hepatic alveolar echinococcosis. A 2 year follow-up with and without flubendazole treatment. Parasite Immunology 6: 329–340PubMedCrossRefGoogle Scholar
  10. 10.
    Al-Kahalidi NW, Barriga OO (1986) Cell-mediated immunity in the prepatent primary infection of dogs with Echinococcus granulosus. Veternary Immunology and Immunopathology 11: 73–82CrossRefGoogle Scholar
  11. 11.
    Blundell-Hasell SK (1974) Cellular immunity to Nippostrongylus brasiliensis in the rat. 11. Influence of infection dose on the production of migration inhibitory factor in vitro. Int Arch Allergy 47: 95–101PubMedCrossRefGoogle Scholar
  12. 12.
    Green BM, Gbakima AA, Albiez, Taylor HR (1985) Humoral and cellular immune responses to Onchocerca volvulus infection in humans. Reviews of Infectious Disease 7: 789–795CrossRefGoogle Scholar
  13. 13.
    Kikuchi Y, Ishikura H, Kikuchi K, Hayasaka H (1989) Pathology of gastric anisakiasis. Gastric Anisakiasis in Japan. Epidemiology, Diagnosis, Treatment. Ishikura H, Namiki M (eds) Springer, Tokyo, pp. 117–127Google Scholar
  14. 14.
    Kikuchi Y, Ueda T, Yoshiki T, Aizawa M, Ishikura H (1967) Experimental immunopathological studies of intestinal anisakiasis. Igaku No Ayumi 62: 731–736 (in Japanese)Google Scholar
  15. 15.
    Kojima K, Koyanagi T, Shiraki K (1966) Pathological studies of anisakiasis (parasitic abscess formation in, gastrointestinal tracts). Jpn J Clin Med 24: 134–143 (in Japanese)Google Scholar
  16. 16.
    Saeki H (1975) Studies on cell-mediated immunity in experimental anisakiasis. J Sapporo Medical College 44: 309–322 (in Japanese)Google Scholar
  17. 17.
    George M, Voughan J (1962) In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Med 111: 514–521Google Scholar
  18. 18.
    Lowry OH, Roserough NJ, Farr AL, Rondall RJ (1956) Protein measurement with Folin phenol reagent. J Bioi Chem 193: 265–275Google Scholar
  19. 19.
    Akao N, Yoshimura H (1989) Latex agglutination test for immunodiagnosis of gastric anisakiasis. Gastric Anisakiasis in Japan. Epidemiology, Diagnosis, Treatment. Ishikura H, Namiki M (eds) Springer, Tokyo, pp 97–102Google Scholar
  20. 20.
    Kikuchi K, Toyokawa O, Nakamura K, Ishiyama H, Yokota H, Sato H, Natori T, Ishikura H, Aizawa M (1970) Immunopathology of experimental anisakiasis. Minophagen Medical Review 15: 54–58 (in Japanese)Google Scholar
  21. 21.
    Saeki H, Mizugaki H, Ishikura H, Hayasaka H (1972) Immunological studies on Anisakiasis 11. Participation of immune response in host-tissue reaction and destruction of parasite bodies. Hokkaido J Med Sci 47: 541–550 (in Japanese)Google Scholar
  22. 22.
    Larsh JE Jr, Weatherly NF (1974) Studies on delayed (cellular) hypersensitivity in mice infected with Trichinella spiralis 1X Delayed dermal sensitivity in artificially sensitized donors. J Parasitology 60: 93–98CrossRefGoogle Scholar
  23. 23.
    Dumonde DC, Wolstencroft RA, Panayi GS, Matthew M, Morley J, Howson WT (1969) “Lymphokines”: Non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature 224: 38–42PubMedCrossRefGoogle Scholar
  24. 24.
    David JR, Schlossman SF (1968) Immunochemical studies on the specificity of cellular hypersensitivity. J Exptl Med 128: 145–1458CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1990

Authors and Affiliations

  • Y. Kikuchi
  • H. Saeki
  • H. Ishikura

There are no affiliations available

Personalised recommendations