Immunodiagnosis for Anisakiasis with Detection of IgE Antibody to Human Anisakiasis by Enzyme-Linked Immunosorbent Assay

  • Y. Takemoto
  • M. Tsuji
  • Y. Iwanaga


Studies on the production of reaginic antibodies in infections of Anisakis larvae in man, have shown that allergic reactions caused by infection of Anisakis larvae were due to Type-I allergic reactions in experimental animals [1], while larval re-infection was expected to induce an allergic especially Type-I reaction. Therefore the diagnosis of anisakiasis by enzyme-linked immnosorbent assay (ELISA) has used the values of IgE in patient sera.


Patient Seron Control Seron McIlvain Buffer Crude Antigen Precipitin Band 
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  1. 1.
    Sato Y, Yamashita T, Otsuru M, Suzuki T, Asaishi T, Nishino C (1975) Studies on the etiologic mechanism of anisakiasis. 1. The anaphylactic reaction of digestive tract to the worm extracts. Jpn J Parasitol 24: 192–202 (in Japanese)Google Scholar
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    Tsuji M (1974) On the immunoelectrophoresis for helminthological researches. Jpn J Parasitol 23: 335–345 (in Japanese)Google Scholar
  3. 3.
    Yoshimura H, Kondo K, Onishi Y, Akao N (1983) Studies on immunodiagnosis of the diseases by heterotopic parasitism of helminthes. Report for Scientific Research (No. 56750159) for the Ministry of Education, pp. 13–27 (personal publication, in Japanese)Google Scholar

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© Springer-Verlag Tokyo 1990

Authors and Affiliations

  • Y. Takemoto
  • M. Tsuji
  • Y. Iwanaga

There are no affiliations available

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