Monoclonal Antibody, Intradermal Reaction, and Sarles ‘Phenomenon

  • S. Takahashi
  • H. Ishikura
  • H. Hayasaka


There have been a considerable number of reports in which the antigens of Anisakis simplex larvae were analyzed by using xenogenic serum. However, it is still difficult to detect specific antigens of the worm because of the high degree of cross reactivities of the antiserum with other nematodes such as Pseudoterranova decipiens larva. The recent success with monoclonal antibodies has led to the production of very specific antibodies in many areas of parasitology. In the present study, we developed hybridomas to obtain monoclonal antibodies for the purpose of detecting the specific antigens present in Anisakis larvae. In this attempt, it has become possible to detect specific epitopes of larvae, distinguishing them from other nematodes, and to analyze the corresponding antigens biochemically. It is obviously important to detect specific antigens of Anisakis larvae in the study of the taxonomic features of anisakid species and the pathological aspects ofanisakiasis. This approach may also lead to improvement in the specificity of the enzyme-linked immunosorbent assay (ELISA) as a serodiagnostic test for anisakiasis.


Monoclonal Antibody Specific Antigen Cross Reactivity Echinococcus Multilocularis Plasmodium Knowlesi 
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© Springer-Verlag Tokyo 1989

Authors and Affiliations

  • S. Takahashi
  • H. Ishikura
  • H. Hayasaka

There are no affiliations available

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