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Monoclonal Antibody, Intradermal Reaction, and Sarles ‘Phenomenon

  • S. Takahashi
  • H. Ishikura
  • H. Hayasaka

Abstract

There have been a considerable number of reports in which the antigens of Anisakis simplex larvae were analyzed by using xenogenic serum. However, it is still difficult to detect specific antigens of the worm because of the high degree of cross reactivities of the antiserum with other nematodes such as Pseudoterranova decipiens larva. The recent success with monoclonal antibodies has led to the production of very specific antibodies in many areas of parasitology. In the present study, we developed hybridomas to obtain monoclonal antibodies for the purpose of detecting the specific antigens present in Anisakis larvae. In this attempt, it has become possible to detect specific epitopes of larvae, distinguishing them from other nematodes, and to analyze the corresponding antigens biochemically. It is obviously important to detect specific antigens of Anisakis larvae in the study of the taxonomic features of anisakid species and the pathological aspects ofanisakiasis. This approach may also lead to improvement in the specificity of the enzyme-linked immunosorbent assay (ELISA) as a serodiagnostic test for anisakiasis.

Keywords

Monoclonal Antibody Specific Antigen Cross Reactivity Echinococcus Multilocularis Plasmodium Knowlesi 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 256: 495–497PubMedCrossRefGoogle Scholar
  2. 2.
    Takahashi S, Sato N, Sato T, Takami T, Mukaiya M, Yagihashi A, Tsurushiin M, Hayasaka H, Kikuchi K, Ishikura H (1986) Detection of anti-Anisakis larvae antibodies using micro-ELISA method. Igakunoayumi 136: 691–692Google Scholar
  3. 3.
    Danforth HD (1982) Development of hybridoma-produced antibodies directed against Eimeria tenella and E. mitis. J Parasitol 68: 392–397CrossRefGoogle Scholar
  4. 4.
    De Ibarra AAL (1982) Monoclonal antibodies to Leishmania tropica major: specificities and antigen location. Parasitology 85: 523–531PubMedCrossRefGoogle Scholar
  5. 5.
    Gowe JS (1983) All metacyclic variable antigen types of Trypanosoma conglence identified using monoclonal antibodies. Nature 306: 389–391CrossRefGoogle Scholar
  6. 6.
    Ortega-Pierres G (1984) Protection against Trichinella spiralis induced by a monoclonal antibody that promotes killing of newborn larvae by granulocytes. Parasit Immunol 6: 275–284CrossRefGoogle Scholar
  7. 7.
    Epstein N (1981) Monoclonal antibodies against a specific surface determinants on malarial (Plasmodium knowlesi) merzoites block erythrocyte invasion. J Immunol 127: 212–217PubMedGoogle Scholar
  8. 8.
    Agatsuma T (1981) Electrophoretic studies on glucosephosphatase isomerase and phosphoglucomutase in two types of Anisakis larvae. Int J Parasitol 12: 35–39Google Scholar
  9. 9.
    Suzuki T (1968) Immunodiagnositic studies on anisakiasis. Jpn J Parasitol 17: 213–220Google Scholar
  10. 10.
    Asaishi K (1974) Antigenic analysis of Anisakis larva and application of fluorescent antibody technique to histological diagnosis of anisakiasis. Sapporo Med J 43: 104–120Google Scholar
  11. 11.
    Matthews B (1984) The source, release and specificity of proteolytic enzyme activity produced by Anisakis simplex larvae in vitro. J Helminth 58: 175–185CrossRefGoogle Scholar
  12. 12.
    Ruitenberg EJ (1971) Histochemical properties of the excretory organ of Anisakis sp. larva. J Parasitol 6: 9–13Google Scholar
  13. 13.
    Hayasaka H, Ishikura H, Miyagi H, Ueno T, Utsumi A, Sato Y (1968) Immunologi-cal study on anisakiasis (1) on its intracutaneous reaction. J Jpn Soc Clin Surg 29: 81–87 (in Japanese)Google Scholar
  14. 14.
    Suzuki T, Shiraki T, Sekino S, Osturu M (1970) Studies on the immunological diagnosis of anisakiasis 3 intradermal test with purified antigen. Jpn J Parasitol 19: 1–9 (in Japanese)Google Scholar
  15. 15.
    Nishino T (1977) Epidemiological studies on anisakiasis. Sapporo Med J 46: 73–88 (in Japanese)Google Scholar
  16. 16.
    Taliaferro WH, Sarles MP (1939) The cellular reaction in skin, lungs and intestine of normal and immune rats after infection with Nippostrongyrus muris. J Infect Dis 64: 157–192CrossRefGoogle Scholar
  17. 17.
    Morishita Y, Nishimura T (1968) Sarles phenomenon on Anisakis larvae. Jpn J Parasitol 17: 30 (in Japanese)Google Scholar
  18. 18.
    Hayasaka H, Mizugaki H, Asaishi K, Takagi R, Iwano H, Ishikura H, Aizawa M (1970) Immunological reaction of intestinal anisakiasis—especially on Sarles phenomenon. Minophagen Med Review 15: 54–58 (in Japanese)Google Scholar

Copyright information

© Springer-Verlag Tokyo 1989

Authors and Affiliations

  • S. Takahashi
  • H. Ishikura
  • H. Hayasaka

There are no affiliations available

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