Monoclonal Antibody, Intradermal Reaction, and Sarles ‘Phenomenon
There have been a considerable number of reports in which the antigens of Anisakis simplex larvae were analyzed by using xenogenic serum. However, it is still difficult to detect specific antigens of the worm because of the high degree of cross reactivities of the antiserum with other nematodes such as Pseudoterranova decipiens larva. The recent success with monoclonal antibodies has led to the production of very specific antibodies in many areas of parasitology. In the present study, we developed hybridomas to obtain monoclonal antibodies for the purpose of detecting the specific antigens present in Anisakis larvae. In this attempt, it has become possible to detect specific epitopes of larvae, distinguishing them from other nematodes, and to analyze the corresponding antigens biochemically. It is obviously important to detect specific antigens of Anisakis larvae in the study of the taxonomic features of anisakid species and the pathological aspects ofanisakiasis. This approach may also lead to improvement in the specificity of the enzyme-linked immunosorbent assay (ELISA) as a serodiagnostic test for anisakiasis.
KeywordsMonoclonal Antibody Specific Antigen Cross Reactivity Echinococcus Multilocularis Plasmodium Knowlesi
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- 2.Takahashi S, Sato N, Sato T, Takami T, Mukaiya M, Yagihashi A, Tsurushiin M, Hayasaka H, Kikuchi K, Ishikura H (1986) Detection of anti-Anisakis larvae antibodies using micro-ELISA method. Igakunoayumi 136: 691–692Google Scholar
- 8.Agatsuma T (1981) Electrophoretic studies on glucosephosphatase isomerase and phosphoglucomutase in two types of Anisakis larvae. Int J Parasitol 12: 35–39Google Scholar
- 9.Suzuki T (1968) Immunodiagnositic studies on anisakiasis. Jpn J Parasitol 17: 213–220Google Scholar
- 10.Asaishi K (1974) Antigenic analysis of Anisakis larva and application of fluorescent antibody technique to histological diagnosis of anisakiasis. Sapporo Med J 43: 104–120Google Scholar
- 12.Ruitenberg EJ (1971) Histochemical properties of the excretory organ of Anisakis sp. larva. J Parasitol 6: 9–13Google Scholar
- 13.Hayasaka H, Ishikura H, Miyagi H, Ueno T, Utsumi A, Sato Y (1968) Immunologi-cal study on anisakiasis (1) on its intracutaneous reaction. J Jpn Soc Clin Surg 29: 81–87 (in Japanese)Google Scholar
- 14.Suzuki T, Shiraki T, Sekino S, Osturu M (1970) Studies on the immunological diagnosis of anisakiasis 3 intradermal test with purified antigen. Jpn J Parasitol 19: 1–9 (in Japanese)Google Scholar
- 15.Nishino T (1977) Epidemiological studies on anisakiasis. Sapporo Med J 46: 73–88 (in Japanese)Google Scholar
- 17.Morishita Y, Nishimura T (1968) Sarles phenomenon on Anisakis larvae. Jpn J Parasitol 17: 30 (in Japanese)Google Scholar
- 18.Hayasaka H, Mizugaki H, Asaishi K, Takagi R, Iwano H, Ishikura H, Aizawa M (1970) Immunological reaction of intestinal anisakiasis—especially on Sarles phenomenon. Minophagen Med Review 15: 54–58 (in Japanese)Google Scholar