The Application of Immunohistochemistry in Assessment of Cellular Proliferation

  • C. C.-W. Yu
  • A. L. Woods
  • D. A. Levison


The use of immunohistochemical techniques enables the visualisation of specific cell cycle related antigens in tissue, with the important advantage of preserving the spatial orientation of cells. Antibodies may recognise endogenous cell cycle related molecules occurring naturally within the tissue to be studied, or exogenous substances, such as bromodeoxyuridine (BUdR), a thymidine analogue incorporated during the S (DNA synthetic) phase of the cell cycle, which may be introduced by in vivo administration (see Chap. 7) or in vitro incubation. There is a wide range of targets for these antibodies, most of which are cell cycle associated antigens, but it is also possible that markers of quiescent and non-proliferating cells and markers of transition from the quiescent to the proliferating state may convey information about proliferative activity (Hall and Woods 1990).


Proliferate Cell Nuclear Antigen Tritiated Thymidine Thymidine Labelling Index Proliferate Cell Nuclear Antigen Labelling Index Proliferate Cell Nuclear Antigen Gene 
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© Springer-Verlag London Limited 1992

Authors and Affiliations

  • C. C.-W. Yu
  • A. L. Woods
  • D. A. Levison

There are no affiliations available

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