Effects of Feeding Levels and Body Weight Loading on Muscle Size and Visceral Organ Sizes in Rats

  • Kaoru Tachiyashiki
  • Kazuhiko Imaizumi
Conference paper


The effects of feeding levels and body weight loading on the soleus muscle size and visceral organ sizes in rats were studied by using the whole-body suspension technique. Body suspensions in the free-feeding and chowrestraint conditions were maintained for 18 and 11 days, respectively. The rats were divided into three groups: cage control (CON), body suspended (BS), and BS plus continuous weight-bearing of the hind limbs on the floor of the cage (BSW). In the free-feeding conditions, the body weights of the BS and BSW rats decreased during the first 4 days. The initial loss of weight correlated with a reduced chow intake during this period. In the chow-restraint conditions, however, the body weight of BS and BSW rats decreased with prolongation of the suspension period, indicating that factors other than chow intake participated in the weight decrease. In both feeding conditions, the adrenal weights of the BS and BSW rats were 2 times higher than those of CON rats, suggesting that suspension stress may produce adrenal hypertrophy. The rectal temperatures of BS and BSW rats were also significantly higher than those of CON rats, showing that a higher metabolic state may be induced by suspension. In both feeding conditions, the weights and protein concentrations of soleus muscles of BSW rats were significantly higher than those of BS rats, suggesting that the weight bearing by the soleus muscles during suspension may maintain the size and protein levels of muscles.


Soleus Muscle Muscle Size Hindlimb Suspension Feeding Level Denim Material 
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  1. 1.
    Booth FW (1977) Time course of muscular atrophy during immobilization of hindlimbs in rats. J Appl Physiol: Respirat Environ Exercise Physiol 43:656–661Google Scholar
  2. 2.
    Kondo H, Miura M, Nakagaki I, Sasaki S, Itokawa Y (1992) Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization. Am J Physiol 262 (Endocrinol Metab 25):E583–E590PubMedGoogle Scholar
  3. 3.
    MacDougal JD, Ward GR, Sale DG, Sutton JR (1977) Biochemical adaptation of human skeletal muscle to heavy resistance training and immobilization. J Appl Physiol: Respirat Environ Exercise Physiol 43:700–703Google Scholar
  4. 4.
    Conbertino VA, Keil LC, Greenleaf JE (1983) Plasma volume, renin, and vasopressin responses to graded exercise after training. J Appl Physiol: Respirat Environ Exercise Physiol 54:508–514Google Scholar
  5. 5.
    Oganov VS, Patapov AN (1976) On the mechanisms of changes in skeletal muscles in the weightless environment. Life Sci Space Res 19:137–143Google Scholar
  6. 6.
    Ilyina-Kakueva EI, Portugalov VV, Krivenkova NP (1976) Space flight effects on the skeletal muscle of rats. Aviat Space Environ Med 47:700–703PubMedGoogle Scholar
  7. 7.
    Yokogoshi H, Takase S, Goda T, Hoshi T (1990) Effects of suspension hypokinesia/ hypodynamia on the body weight and nitrogen balance in rats fed with various protein concentration. Agric Biol Chem 54:779–789CrossRefGoogle Scholar
  8. 8.
    Thomason DB, Booth FW (1990) Atrophy of the soleus muscle by hindlimb unweighting. J Appl Physiol 68:1–12PubMedCrossRefGoogle Scholar
  9. 9.
    Darr KC, Schultz E (1989) Hindlimb suspension suppresses muscle growth and satellite cell proliferation. J Appl Physiol 67:1827–1834PubMedGoogle Scholar
  10. 10.
    Booth FW, Thomason DB (1991) Molecular and cellular adaptation of muscle in response to exercise: Perspectives of various model. J Appl Physiol 71:541–585Google Scholar
  11. 11.
    Cooper RR (1972) Alterations during immobilization and regeneration of skeletal muscle in cats. J Bone Jt Surg Am 54-A:919–953Google Scholar
  12. 12.
    Musacchia XJ, Deavers DR, Meininger GA, Davis TP (1980) A model for hypokinesia: Effects on muscle atrophy in the rat. J Appl Physiol 48:479–486PubMedGoogle Scholar
  13. 13.
    Morey-Holten E, Wronski TJ (1981) Animal models for simulating weightlessness. Physiologist 24[Suppl VI]:S45-S48Google Scholar
  14. 14.
    Witzman FA, Kim DH, Fitt RH (1983) Effect of hindlimb immobilization on the fatigability of skeletal muscle. J Appl Physiol 54:1242–1248Google Scholar
  15. 15.
    Ohira Y (1989) Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles. Jpn J Physiol 39:21–31PubMedCrossRefGoogle Scholar
  16. 16.
    Baranski S, Kwarecki K, Szmigielski S, Rozynski J (1971) Histochemistry of skeletal muscle fibers in rats undergoing long term experimental hypokinesia. Follia Histochem Cytochem 9:381–386Google Scholar
  17. 17.
    Imaizumi K, Tachiyashiki K, Shirata M (1992) Hindlimb suspension-induced changes of skeletal muscles and physiological functions in rats. Jpn J Physiol 42 [Suppl]:S255Google Scholar
  18. 18.
    Imaizumi K, Tachiyashiki K, Jikihara K (1993) Responses of the sizes of visceral organs and muscles to whole body suspension, and recovery in rats. Jpn J Physiol 43 [Suppl]:S324Google Scholar
  19. 19.
    Hayase K, Yokogoshi H (1991) Effects of suspension hypokinesia/hypodynamia on tissue protein turnover in rats. Jpn J Physiol 41:473–482PubMedCrossRefGoogle Scholar
  20. 20.
    Ohira Y, Jiang B, Roy RR, Oganov V, Ilyina-Kakueva EI, Marini JF, Edgerton VR (1992) Rat soleus muscle fiber responses to 14 days of spaceflight and hindlimb suspension. J Appl Physiol 73[Suppl]:51S–57SPubMedGoogle Scholar
  21. 21.
    Jiang B, Ohira Y, Roy RR, Nguyen Q, Ilyina-Kakueva EI, Oganov V, Edgerton VR (1992) Adaptation of fibers in fast-twitch muscles of rats to spaceflight and hindlimb suspension. J Appl Physiol 73[Suppl]:58S–65SPubMedGoogle Scholar
  22. 22.
    Thomason DB, Morrison PR, Oganov V, Ilyina-Kakueva EI, Booth FW, Baldwin KM (1992) Altered actin and myosin expression in muscle during exposure to microgravity. J Appl Physiol 73[Suppl]:90S–93SPubMedGoogle Scholar
  23. 23.
    Tachiyashiki K, Imaizumi K (1992) Lowering and delaying actions of bovine bile on plasma ethanol levels in rats. J Nutr Sci Vitaminol 38:69–82PubMedCrossRefGoogle Scholar
  24. 24.
    Tachiyashiki K, Imaizumi K (1993) Effects of vegetable oils and C18-unsaturated fatty acids on plasma ethanol levels and gastric emptying in ethanol-administered rats. J Nutr Sci Vitaminol 39:163–176PubMedCrossRefGoogle Scholar
  25. 25.
    Hauschka EO, Roy RR, Edgerton VE (1987) Size and metabolic properties of single muscle fibers in rat soleus after hindlimb suspension. J Appl Physiol 62: 2338–2347PubMedGoogle Scholar
  26. 26.
    The Physiological Society of Japan (1990) Guiding principle for the care and use of animals in the field of physiological sciences. The Physiological Society of Japan, TokyoGoogle Scholar
  27. 27.
    Imaizumi K, Tachiyashiki K, Sekiya M (1990) Quantitative analysis of skeletal muscles in dystrophyic mice as a model of non-exercise muscle atrophy. In: Kaneko M (ed) Fitness for the aged, disabled and industrial worker. Human Kinetics, Champaign, pp 169–177Google Scholar
  28. 28.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1953) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  29. 29.
    Ballard FJ, Tomas FM (1983) 3-Methylhistidine as a measure of skeletal muscle protein breakdown in human subject: The case for its continued use. Clin Sci 65:209–215PubMedGoogle Scholar
  30. 30.
    Tsuchiya K, Matsumoto I, Kosaka M, Aikawa T (1992) Rise in core temperature and changes in plasma catecholamines level during restrained stress in rats. Jpn J Physiol 42[Suppl]:S291Google Scholar
  31. 31.
    Goldberg AL, Goodman HM (1969) Relationship between cortisone and muscle work in determining muscle size. J Physiol (London) 200:667–675Google Scholar
  32. 32.
    Odedra BR, Bates PC, Millward DJ (1983) Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver. Biochem J 214:617–627PubMedGoogle Scholar

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© Springer-Verlag Tokyo 1994

Authors and Affiliations

  • Kaoru Tachiyashiki
  • Kazuhiko Imaizumi

There are no affiliations available

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