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Uridine is a Marker of Viability in Myocardial Ischemia

  • Hiroyuki Yaoita
  • Alan J. Fischman
  • Hiroshi Ohtani
  • H. William Strauss
  • Michito Kanke
  • Tsukasa Asakura
  • Tomiyoshi Saito
  • Nobuaki Tsuchihashi
  • Yukio Maruyama

Abstract

To test the hypothesis that uridine may be a marker of viability in myocardial ischemia, myocardial uptake of uridine was compared to that of deoxyglucose, a conventional marker of viability, in experimental myocardial ischemia. Fifteen male Wistar rats had occlusion of the left coronary artery for 5, 10, or 60 min. Four h after reperfusion, 60 uCi of 3H uridine and 2 uCi of 14C deoxyglucose were administered intravenously and the animals were sacrificed 45 min later. Two min before sacrifice, 100 uCi of thallium-201 was injected intravenously as a marker of myocardial perfusion. After sacrifice, the heart was removed and the left ventricle was sliced in a breadloaf fashion into 20 segments. Thallium-201 radioactivity in each segment was determined with a gamma counter; 1 month later 3H and 14C radioactivity was determined with a beta counter. Then the target to normal ratio (T/N) of the myocardial segment was calculated. Triphenyl tetrazolium chloride (TTC) staining was examined in neighboring myocardial specimens.

Keywords

Myocardial Perfusion Left Coronary Artery Gamma Counter Myocardial Segment Myocardial Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag Tokyo 1993

Authors and Affiliations

  • Hiroyuki Yaoita
    • 1
  • Alan J. Fischman
    • 2
  • Hiroshi Ohtani
    • 2
  • H. William Strauss
    • 2
  • Michito Kanke
  • Tsukasa Asakura
  • Tomiyoshi Saito
  • Nobuaki Tsuchihashi
  • Yukio Maruyama
    • 1
  1. 1.First Department of Internal MedicineFukushima Medical CollegeFukushimaJapan
  2. 2.Massachusetts General HospitalBostonUSA

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