Suspension Culture of Insect Cells in a Spin-Filter Bioreactor for Recombinant Protein Production

  • H. R. Kim
  • J. H. Oh
  • J. M. Yang
  • S. K. Kang
  • H. H. Yoon
  • I. S. Chung
Conference paper


Protein expression systems based on the Autographa californica nuclear polyhedrosis virus (AcNPV) have wide applicability as an alternative to prokaryotic or other eukaryotic expression system (1-5). However, the application of the baculovirus expression systems has been limited by difficulties in scale-up of insect cell culture (6-7). Therefore, it is quite natural that scale-up of insect cell culture would be a challenging scientific and technological undertaking to ensure the efficient production of various significant products for human health and medicine.


Insect Cell Recombinant Protein Production Perfusion Culture Cell Retention Viable Cell Density 
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Copyright information

© Springer-Verlag Tokyo 1992

Authors and Affiliations

  • H. R. Kim
    • 1
  • J. H. Oh
    • 1
  • J. M. Yang
    • 2
  • S. K. Kang
    • 3
  • H. H. Yoon
    • 4
  • I. S. Chung
    • 1
  1. 1.Department of Genetic EngineeringKyung Hee UniversityKorea
  2. 2.Department of Genetic EngineeringSungkyunkwan UniversitySeoulKorea
  3. 3.Department of Natural FibersSeoul National UniversitySeoulKorea
  4. 4.Department of Chemical EngineeringKyungwon UniversitySuwonKorea

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