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Production of t-PA by Recombinant Mouse Fibroblast Cell Cultures in a Perfusion Bioreactor

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Biochemical Engineering for 2001

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Abstract

As heart disease is known to be one of the leading cause of human death, t-PA(tissue-type plasminogen activator) has been received great attention as a promising thrombolytic agent and also as one of the first commercial products via the recombinant DNA-technology. Breaking up blood clot is the essential treatment of restoring the normal flow of blood in the acute myocardial infarction cases [1]. Thrombolytic agents activates plasminogen to form plasmin, which dissolves fibrin-the main component of blood clots. Plasminogen is a pro-enzyme that naturally exists in blood as free form and/or plasminogen-fibrin complex by binding to blood clots, more specifically fibrin.

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References

  1. Chien K, Meidell R, Gerard R (1987) Tissue plasminogen activator: From molecular biology to myocardial infarction. Amer. J. of Med. Sci., 292, 201–207.

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  2. Geiger M, Binder B (1987) Tissue-type plasminogen activator and urokinase: Differences in the reaction pattern with active-site titrant 4-methyumbelliferyl-p guanidinobenzoate hydrochloride. Biochimica Biophysica Acta, 912, 34–40

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  3. Kretzmer G, Scrugerl K (1991) Response of mammalian cells to shear stress. Appl Microbiol Biotechnol, 34, 613–616.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Kangweon National University, Chuncheon, 200-701, Korea

    Gyu Heon Cho

  2. Eugene Tech International Inc, 1000C Lake St, Ramsey, NJ, 07446, USA

    Young Jun Kim

Authors
  1. Gyu Heon Cho
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  2. Young Jun Kim
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Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113, Japan

    Shintaro Furusaki

  2. The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama, 351-01, Japan

    Isao Endo (Head of Chemical Engineering Laboratory) (Head of Chemical Engineering Laboratory)

  3. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-01, Japan

    Ryuichi Matsuno

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© 1992 Springer-Verlag Tokyo

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Cho, G.H., Kim, Y.J. (1992). Production of t-PA by Recombinant Mouse Fibroblast Cell Cultures in a Perfusion Bioreactor. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_88

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  • DOI: https://doi.org/10.1007/978-4-431-68180-9_88

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68182-3

  • Online ISBN: 978-4-431-68180-9

  • eBook Packages: Springer Book Archive

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