Abstract
Aqualysin I (AQI) is a subtilisin-type serine protease which is secreted into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. AQI is considerably heat-stable and resistant to denaturing treatments in comparison with other microbial serine proteases. Terada et al. previously reported the production of the enzymatically active AQI by expressing the AQI gene under the control of the tac promoter in Escherichia coli [1]. In this study, to achieve efficient production of AQI, batch and fed-batch cultures of E.coli TG1 transformed with plasmid pAQN containing the AQI gene were investigated.
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References
Terada I, Kwon S, Miyata Y, Matsuzawa H, Ohta T (1990) Unique Precursor Structure of an Extracellular Protease, Aqualysin I, with NH2- and COOH-terminal Pro-sequences and Its Processing in Escherichia coli, J Biol Chem 265: 6576–6581
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© 1992 Springer-Verlag Tokyo
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Sakamoto, S. et al. (1992). Production of Thermophilic Protease (Aqualysin I) by Fed-Batch Cultures of Recombinant Escherichia coli. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_50
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DOI: https://doi.org/10.1007/978-4-431-68180-9_50
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68182-3
Online ISBN: 978-4-431-68180-9
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