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Growth and Induction Effects on the Expression of rhTNF-ß

  • K. W. Y. Mak
  • M. G. S. Yap
  • Y. L. Huang
  • W. K. Teo

Abstract

The Trp promoter has been widely used in recombinant technology for the over expression of foreign proteins in E. coli [1,2]. The conditions for optimal expression vary considerably among these reports. In this study, the effects of culture conditions, such as medium composition, temperature and concentrations of tryptophan and 3-β-indoleacrylic acid (IAA) on the expression of recombinant human tumour necrosis factor-β (rhTNF-β) in E. coli K12 HB101 pCG402 were investigated. The expression of the protein is under the control of a modified trp promoter, which lacks the attenuator [3].

Keywords

Complex Medium Casein Hydrolysate High Cell Density Culture High Specific Growth Rate Define Medium 
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Copyright information

© Springer-Verlag Tokyo 1992

Authors and Affiliations

  • K. W. Y. Mak
    • 1
  • M. G. S. Yap
    • 1
    • 2
  • Y. L. Huang
    • 2
  • W. K. Teo
    • 1
  1. 1.Department of Chemical EngineeringNational University of SingaporeSingapore
  2. 2.Bioprocessing Technology UnitNational University of SingaporeSingapore

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