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Growth and Induction Effects on the Expression of rhTNF-ß

  • Conference paper
Biochemical Engineering for 2001

Abstract

The Trp promoter has been widely used in recombinant technology for the over expression of foreign proteins in E. coli [1,2]. The conditions for optimal expression vary considerably among these reports. In this study, the effects of culture conditions, such as medium composition, temperature and concentrations of tryptophan and 3-β-indoleacrylic acid (IAA) on the expression of recombinant human tumour necrosis factor-β (rhTNF-β) in E. coli K12 HB101 pCG402 were investigated. The expression of the protein is under the control of a modified trp promoter, which lacks the attenuator [3].

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References

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Authors and Affiliations

  1. Department of Chemical Engineering, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore

    K. W. Y. Mak, M. G. S. Yap & W. K. Teo

  2. Bioprocessing Technology Unit, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore

    M. G. S. Yap & Y. L. Huang

Authors
  1. K. W. Y. Mak
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  2. M. G. S. Yap
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  3. Y. L. Huang
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  4. W. K. Teo
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Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113, Japan

    Shintaro Furusaki

  2. The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama, 351-01, Japan

    Isao Endo (Head of Chemical Engineering Laboratory) (Head of Chemical Engineering Laboratory)

  3. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-01, Japan

    Ryuichi Matsuno

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© 1992 Springer-Verlag Tokyo

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Mak, K.W.Y., Yap, M.G.S., Huang, Y.L., Teo, W.K. (1992). Growth and Induction Effects on the Expression of rhTNF-ß. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_49

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  • DOI: https://doi.org/10.1007/978-4-431-68180-9_49

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68182-3

  • Online ISBN: 978-4-431-68180-9

  • eBook Packages: Springer Book Archive

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