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Production of Recombinant HTLV-I Envelope Protein

  • M. L. Hong
  • M. G. S. Yap
  • B. T. Koh
  • L. Chan
Conference paper

Abstract

Optimization of the culture and induction conditions of fermentation to produce recombinant proteins expressed in bacteria is an important area of research. The human T-cell lymphotropic virus type-I (HTLV-I) envelope protein (p21E) expressed in E. coli K12 MZ1 pKS400 is used as an antigen in a diagnostic assay to detect serological responses to HTLV-I infections [1,2]. In this study, the expression of p21E under varying medium and induction conditions were investigated. A 2-L fed-batch fermentation was performed in which a cell density of 30 g DCM/L and a yield of p21E of 65 mg/g DCM (32% of the total protein) were obtained.

Keywords

Complex Medium Envelope Protein Induction Condition Serological Response Early Induction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Tokyo 1992

Authors and Affiliations

  • M. L. Hong
    • 1
  • M. G. S. Yap
    • 1
    • 2
  • B. T. Koh
    • 2
  • L. Chan
    • 3
  1. 1.Department of Chemical EngineeringNational University of SingaporeSingapore
  2. 2.Bioprocessing Technology UnitNational University of SingaporeSingapore
  3. 3.Diagnostic Biotechnology Pte. LtdSingapore Science ParkSingapore

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