Production of Recombinant HTLV-I Envelope Protein
Optimization of the culture and induction conditions of fermentation to produce recombinant proteins expressed in bacteria is an important area of research. The human T-cell lymphotropic virus type-I (HTLV-I) envelope protein (p21E) expressed in E. coli K12 MZ1 pKS400 is used as an antigen in a diagnostic assay to detect serological responses to HTLV-I infections [1,2]. In this study, the expression of p21E under varying medium and induction conditions were investigated. A 2-L fed-batch fermentation was performed in which a cell density of 30 g DCM/L and a yield of p21E of 65 mg/g DCM (32% of the total protein) were obtained.
KeywordsComplex Medium Envelope Protein Induction Condition Serological Response Early Induction
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