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Biochemical Engineering for 2001 pp 103–105Cite as

The Effect of Amphopathics on the Properties of Enzyme (Lipase, Cholinesterase, Cellulase)

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Abstract

The interaction ways between enzyme and amphopathics depend on the polarity of reaction medium. 1. during the esterification catalyzed by lipase in benzen, the activity of lipase was incresed 3 folds by adding amphopathics A, B, C. 2. The thermostability and resistence to pepsin hydrolysis of cholinesterase were improved by adding Tween 20, Tween 80, Alkeslguhr to aquous reaction. 3. The activity of cellulose was inhibited by 10% ethanol. By adding Tween 20. Tween 80. BRIJ 35, cellulase relieved the inhibition of ethanol and its half-life increased 2-3. folds the results showed that amphopathics affected the properties of enzyme by different ways, but this effect was intimately related to conc. and structure of amophopatics.

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References

  1. Ellman G. L. et al (1961) Biochem Pharmacol 7: 88

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  2. G. L. Miller (1959) Anal Chem 37: 4426

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Author information

Authors and Affiliations

  1. National Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023, China

    Cao Shu-gui, Feng Yan, Yu Tao & Yang Hong

Authors
  1. Cao Shu-gui
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  2. Feng Yan
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  3. Yu Tao
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  4. Yang Hong
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  5. Liu Shi-jie
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Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113, Japan

    Shintaro Furusaki

  2. The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama, 351-01, Japan

    Isao Endo (Head of Chemical Engineering Laboratory) (Head of Chemical Engineering Laboratory)

  3. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-01, Japan

    Ryuichi Matsuno

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© 1992 Springer-Verlag Tokyo

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Cite this paper

Shu-gui, C., Yan, F., Tao, Y., Hong, Y., Shi-jie, L. (1992). The Effect of Amphopathics on the Properties of Enzyme (Lipase, Cholinesterase, Cellulase). In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_25

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  • DOI: https://doi.org/10.1007/978-4-431-68180-9_25

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68182-3

  • Online ISBN: 978-4-431-68180-9

  • eBook Packages: Springer Book Archive

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