Maximizing a Lysine Production in Fed-Batch Culture by Controlling the Specific Growth Rate Under Leucine Limited Conditions

To attain maximum production in a fed-batch culture, the three steps are usually passed through; i.e. modeling of the process, calculation of the optimal solution, and realization of the solution. Using this approach, any discrepancy between the model and the real process identified from the experiment is one of the problems taken into account.The ease of the optimization calculation is also another aspect which must be considered. From these view points, one of the most appropriate approaches, in which an optimum solution is simple to obtain was proposed previously [1, 2]. In this method, first, the relationship between the specific growth rate, u, and the specific production rate, p, is identified and represented by a model. Then, the optimal strategy of the specific growth rate is obtained by one of optimization methods, such as, the Maximum Principle. Finally, this strategy was realized. Comparing this method with the usual approach to optimization, a simple model with easy optimization but with are latively complex ealization method are the characteristics of the approach introduced here.