Transfer of Isolated Nuclei of Saccharomyces cerevisiae into Anucleated Protoplasts of Nectaromyces species

Abstract

The isolation-fusion technology of protoplasts of microbial cell is a kind of developing technology growing on the basis of the cell fusion with gene technology. In the dissertation, two of the yeast were chosen to study the isolation-fusion technology of protoplasts. One was Saccharomyces cerevisiae which was able to grow in a high temperature (42°C) and rich-alcohol (15%) surrounding and another was Nectaromyces sp. which was able to resist high osmotic pressure (55% glucose). We tried for creating a new way of genetic breeding and providing a method for industrial microbial breeding, as well as setting up an experimental model of transferring isolated nuclei and anucleated system of eukaryotic organisms, to supply a new sort of technology for researching distant hybridization and genetic recombination on the level of molecule or cell.