Protein Separation in the Medium of Organic Solvent by High Electrical Potential
Proteins are the macromolecules containing the biological functions and can be separated by the conventional methods such as sedimentation, filtration, chromatography, electrophoresis and biological affinity . Proteins migrate in an electrical field because of the electrical charges they possess in an aqueous solution. The migration speed of proteins depends on electrical charges of proteins to be separated and the applied voltage between the two plates. The application of high voltage is limited by the heat generation in the aqueous solution. The migration speed of proteins depends on electrical charges of proteins to be separated and the applied voltage between the two plates. The application of high voltage is limited by the heat generation in the aqueous solution. And Haber employed successfully non-aqueous high voltage system in separating organic dyes and called this method as electromolecular propulsion. We can use an organic solvent as a medium instead of buffer solution to minimize heat generation and increase the moving velocity of protein at high applied voltages in the conventional electrophoresis reactor. The effect of organic solvent used as the medium on the activity of proteins was negligible . The activity was protected by the organic solvent for the case that the protein was solved in the acid buffer solution. Although the medium of buffer solution in the electrophoresis cell can be changed with organic solvent, protein should be solved in an aqueous solution to take the electrical charges. In this study we wanted to investigate the mechanism of protein migration in the medium of organic solvent and mode of protein movement.
KeywordsElectrical Charge Heat Generation Propylene Glycol Protein Solution Propylene Carbonate
Unable to display preview. Download preview PDF.
- 2.Haber N (1982) Chemoelectronic mobilization of chemical species in low conductivity fluids — new electrokinetic effects, Biochem. J. 79: 272–276Google Scholar
- 3.Hong H C, Ryu W K, Park J K, Song B H (1991) Protein transport by the paper electrophoresis in organic solvent medium, KIChE J. 29: 457–462Google Scholar
- 5.Yezawa M (1968) Polyelectrolytes, Kagaku Shokan 37: 7Google Scholar