Possible Involvement of Abnormal Cytokinesis and Karyokinesis in the Manifestation of Hepatitis in LEC Rats
In jaundiced LEC rats, the histopathological characteristics of the liver include markedly enlarged hepatocytes with huge nuclei whose DNA content appears to be more than octaploid. At the age of 14 weeks, immediately prior to the onset of hepatitis, the nuclei of hepatocytes often vary in size, and their enlarged nuclei frequently possess several nucleoli. Based upon these observations, we have analyzed nuclei ploidy, the percentage of binucleated cells, and DNA synthesis in the hepatocytes of LEC rats at various ages in order to determine whether abnormal cytokinesis and karyokinesis occur in LEC rats [1, 2].
KeywordsSapporo Medical College Hepatocyte Nucleus Propidium Iodide Solution Label Index BrdU Polyploid Nucleus
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