The LEC Rat pp 81-86 | Cite as

Possible Involvement of Abnormal Cytokinesis and Karyokinesis in the Manifestation of Hepatitis in LEC Rats

  • Yoshinori Fujimoto
  • Michio Mori
  • Minako Nagao


In jaundiced LEC rats, the histopathological characteristics of the liver include markedly enlarged hepatocytes with huge nuclei whose DNA content appears to be more than octaploid. At the age of 14 weeks, immediately prior to the onset of hepatitis, the nuclei of hepatocytes often vary in size, and their enlarged nuclei frequently possess several nucleoli. Based upon these observations, we have analyzed nuclei ploidy, the percentage of binucleated cells, and DNA synthesis in the hepatocytes of LEC rats at various ages in order to determine whether abnormal cytokinesis and karyokinesis occur in LEC rats [1, 2].


Sapporo Medical College Hepatocyte Nucleus Propidium Iodide Solution Label Index BrdU Polyploid Nucleus 
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  1. 1.
    Fujimoto Y, Oyamada M, Hattori A, Takahashi H, Sawaki M, Dempo K, Mori M, Nagao M (1989) Accumulation of abnormally high ploid nuclei in the liver of LEC rats developing spontaneous hepatitis. Jpn J Cancer Res 80:45–50.PubMedCrossRefGoogle Scholar
  2. 2.
    Fujimoto Y, Takahashi H, Dempo K, Mori M, Wirth PJ, Nagao M, Sugimura T (1989) Hereditary hepatitis in LEC rats: Accumulation of abnormally high ploid nuclei. Cancer Detect Prev 14:235–237.PubMedGoogle Scholar
  3. 3.
    Sasaki M, Yoshida MC, Kagami K, Takeichi N, Kobayashi H, Dempo K, Mori M (1985) Spontaneous hepatitis in an inbred strain of Long-Evans rats. Rat News Lett 14:4–6.Google Scholar
  4. 4.
    Yoshida MC, Masuda R, Sasaki M, Takeichi N, Kobayashi H, Dempo K, Mori M (1987) New mutation causing hereditary hepatitis in the laboratory rat. J. Hered 78:828–835.Google Scholar
  5. 5.
    Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 43:506–520.PubMedCrossRefGoogle Scholar
  6. 6.
    Vindelof LL, Christensen IJ, Nissen NI (1983) A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3: 323–327.CrossRefGoogle Scholar
  7. 7.
    Roszell JA, Fredi JL, Irving CC (1978) The development of polyploidy in two classes of rat liver nuclei. Biochim Biophys Acta 519:306–316.PubMedGoogle Scholar
  8. 8.
    Wiest L (1972) The effect of diethylnitrosamine on the distribution of cell classes in the parenchyma of the liver of newborn rats. Eur J Cancer 8:121–125.PubMedGoogle Scholar
  9. 9.
    Brodsky WY, Uryvaeva IV (1977) Cell polyploidy: Its relation to tissue growth and function. Int Rev Cytol 50:275–332.PubMedCrossRefGoogle Scholar
  10. 10.
    LeBouton AV (1976) DNA synthesis and cell proliferation in the simple liver acinus of 10-to 20-day-old rats: Evidence for cell fusion. Anat Rec 184: 679–688.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Yoshinori Fujimoto
    • 1
    • 2
    • 3
  • Michio Mori
    • 1
  • Minako Nagao
    • 2
  1. 1.Department of PathologySapporo Medical CollegeSapporo, 060Japan
  2. 2.Carcinogenesis DivisionNational Cancer Center Research InstituteChuo-Ku, Tokyo, 104Japan
  3. 3.Laboratory of Experimental CarcinogenesisNational Cancer Institute, National Institutes of HealthBethesdaUSA

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