Advertisement

The LEC Rat pp 341-348 | Cite as

Chromosomal Mapping of the Placental Glutathione S-Transferase Gene and Its Expression in Livers of LEC Rats

  • Ryuichi Masuda
  • Michihiro C. Yoshida
  • Motomichi Sasaki

Abstract

The LEC strain rat is a new mutant which suddenly suffers from fulminant hepatitis at about 4 months of age [1, 2]. A crossing test of LEC rats showed that the hepatitis has an autosomal recessive mode of inheritance ruled by a single gene designated hts [3]. About 30% of the animals died of the disease within 1 week after onset. The remaining 70% survived with chronic hepatitis for more than 1 year and developed well-differentiated hepatocellular carcinomas (hepatomas) together with cholangiofibrosis at a remarkably high incidence [4]. Therefore, it is important to clarify what are the characteristics of hepatic lesions in LEC rats.

Keywords

Chronic Hepatitis Hepatic Lesion Fulminant Hepatitis Autosomal Recessive Mode Chemical Hepatocarcinogenesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sasaki M, Yoshida MC, Kagami K, Takeichi N, Kobayashi H, Dempo K, Mori M (1985) Spontaneous hepatitis in an inbred strain of Long-Evans rats. Rat News Lett 14:4–6.Google Scholar
  2. 2.
    Yoshida MC, Masuda R, Sasaki M, Takeichi N, Kobayashi H, Dempo K, Mori M (1987) New mutation causing hereditary hepatitis in the laboratory rat. J Hered 78:361–365.PubMedGoogle Scholar
  3. 3.
    Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) Hereditary hepatitis of LEC rats is controlled by a single autosomal recessive gene. Lab Anim 22:166–169.PubMedCrossRefGoogle Scholar
  4. 4.
    Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) High susceptibility to hepatocellular carcinoma development in LEC rats with hereditary hepatitis. Jpn J Cancer Res 79:828–835.PubMedCrossRefGoogle Scholar
  5. 5.
    Kitahara A, Satoh K, Nishimura K, Ishikawa T, Ruike K, Sato K, Tsuda H, Ito N (1984) Changes in molecular forms of rat hepatic glutathione S-transferase during chemical hepatocarcinogenesis. Cancer Res 44:2698–2703.PubMedGoogle Scholar
  6. 6.
    Sato K, Kitahara A, Satoh K, Ishikawa T, Tatematsu M, Ito N (1984) The placental form of glutathione S-transferase as a new marker protein for preneoplasia in rat chemical hepatocarcinogenesis. Jpn J Cancer Res 75:199–202.Google Scholar
  7. 7.
    Satoh K, Kitahara A, Soma Y, Inaba Y, Hatayama I, Sato K (1985) Purification, induction, and distribution of placental glutathione transferase: A new marker enzyme for preneoplastic cells in the rat chemical hepatocarcinogenesis. Proc Natl Acad Sci USA 82:3964–3968.PubMedCrossRefGoogle Scholar
  8. 8.
    Sugioka Y, Fujii-Kuriyama Y, Kitagawa T, Muramatsu M (1985) Changes in Polypeptide pattern of rat liver cells during chemical hepatocarcinogenesis. Cancer Res 45:365–378.PubMedGoogle Scholar
  9. 9.
    Sugioka Y, Kano T, Okuda A, Sakai M, Kitagawa T, Muramatsu M (1985) Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA. Nucleic Acids Res 13:6049–6057.CrossRefGoogle Scholar
  10. 10.
    Okuda A, Sakai M, Muramatsu M (1987) The structure of the rat glutathione S-transferase P gene and related pseudogenes. J Biol Chem 262:3858–3863.PubMedGoogle Scholar
  11. 11.
    Oyamada M, Dempo K, Fujimoto Y, Takahashi H, Satoh MI, Mori M, Masuda R, Yoshida MC, Satoh K, Sato K (1988) Spontaneous occurrence of placental glutathine S-transferase-positive foci in the livers of LEC rats. Jpn J Cancer Res 79:5–8.PubMedCrossRefGoogle Scholar
  12. 12.
    Masuda R, Yoshida MC, Sasaki M, Okuda A, Sakai M, Muramatsu M (1986) Localization of the gene for glutathione S-transferase P on rat chromosome 1 at band q43. Jpn J Cancer Res 77:1055–1058.PubMedGoogle Scholar
  13. 13.
    Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) A transplantable cell line derived from spontaneous hepatocellular carcinoma of the hereditary hepatitis LEC rat. Jpn J Cancer Res 79:250–254.PubMedCrossRefGoogle Scholar
  14. 14.
    Masuda R, Yoshida MC, Sasaki M (1989) Gene expression of placental glutathione S-transferase in hereditary hepatitis and spontaneous hepatocarcinogenesis of LEC strain rats. Jpn J Cancer Res 80:1024–1027.PubMedCrossRefGoogle Scholar
  15. 15.
    Sato K (1988) Glutathione S-transferases and hepatocarcinogenesis. Jpn J Cancer Res 79:556–572.PubMedCrossRefGoogle Scholar
  16. 16.
    More MA, Fukushima S, Ichihara A, Sato K, Ito N (1986) Intestinal metaplasia and altered enzyme expression in propymitrosamine-induced Syrian hamster cholangiocellular and gallbladder lesions. Virchows Arch [B] 51:29–38.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Ryuichi Masuda
    • 1
  • Michihiro C. Yoshida
    • 1
  • Motomichi Sasaki
    • 2
  1. 1.Chromosome Research Unit, Faculty of ScienceHokkaido UniversitySapporo, 060Japan
  2. 2.Sasaki Cancer InstituteChiyoda-ku, Tokyo, 101Japan

Personalised recommendations