The LEC Rat pp 273-279 | Cite as

Altered Oncogene Expression in Hepatocellular Carcinomas Developing Spontaneously in LEC Rats

  • Minako Nagao
  • Yoshinori Fujimoto
  • Yukihito Ishizaka
  • Katsuhiko Enomoto
  • Hidetoshi Takahashi
  • Michio Mori
  • Takashi Sugimura


LEC rats spontaneously suffer hepatitis at around 4 months after birth, enter the chronic phase of hepatitis, and spontaneously develop hepatocellular carcinomas (HCCs) between 1 – 1.5 years after birth [1]. Although dominant oncogene activation in chemically induced HCCs in rats is generally understood to be relatively rare, for the LEC rat, in which an endogenous causative agent appears to be involved, the situation remains unclear. Ha-ras activation has been found with a very high frequency in both chemically induced and spontaneously induced liver carcinomas of B6C3 F1 mice [2–5], but, with the exception of those induced by aflatoxin B1 [6, 7], any type of activated ras was only rarely observed in chemically induced rat liver tumors [4, 8, 9]. We examined HCCs in LEC rats for Ha-, Ki- and N-ras gene mutations. The Polymerase chain reaction (PCR) was adopted for amplifying discrete specific DNA fragments [10, 11], and the sequences were determined directly.


Hyperplastic Nodule Single Cell Necrosis National Cancer Center Research Institute B6C3FI Mouse Hereditary Hepatitis 
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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Minako Nagao
    • 1
  • Yoshinori Fujimoto
    • 1
  • Yukihito Ishizaka
    • 1
  • Katsuhiko Enomoto
    • 2
  • Hidetoshi Takahashi
    • 2
  • Michio Mori
    • 2
  • Takashi Sugimura
    • 1
  1. 1.Carcinogenesis DivisionNational Cancer Center Research InstituteTokyo, 104Japan
  2. 2.Department of PathologySapporo Medical CollegeSapporo, 060Japan

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