Skip to main content

Identifications of Carbonic Anhydrase III and Triosephosphate Isomerase in the Liver Proteins in LEC Rats on Two-Dimensional Gel Electrophoresis

  • Chapter
Book cover The LEC Rat

Abstract

The two-dimensional gel (2-DG) electrophoresis analysis of total cellular proteins as described by O’Farrell [1] is suitable for investigating certain protein changes which precede hepatitis and/or hepatocellular carcinomas (HCCs).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021.

    PubMed  Google Scholar 

  2. Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106.

    PubMed  CAS  Google Scholar 

  3. Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035–10038.

    PubMed  CAS  Google Scholar 

  4. Lu HS, Yuan PM, Gracy RW (1984) Primary structure of human triosephosphate isomerase. J Biol Chem 259:11958–11968.

    PubMed  CAS  Google Scholar 

  5. Kelly CD, Carter ND, Jeffery S, Edwards YH (1988) Characterisation of cDNA clones for rat muscle carbonic anhydrase III. Biosci Rep 8:401–406.

    Article  PubMed  CAS  Google Scholar 

  6. Carter ND, Shiels A, Jeffery S, Heath R, Wilson CA, Phillips IR, Shephard EA (1984) Hormonal control of carbonic anhydrase III. Ann NY Acad Sci 429: 287–301.

    Article  PubMed  CAS  Google Scholar 

  7. Shiels A, Jeffery S, Phillips IR, Shephard EA, Wilson CA, Carter ND (1983) Sexual differentiation of rat liver carbonic anhydrase III. Biochim Biophys Acta 760:335–342.

    Article  PubMed  CAS  Google Scholar 

  8. Ramaley JA (1982) The neuroendocrinology of puberty. In: Vernadakis A, Timiras P (eds) Hormones in development and aging. Spectrum, New York pp 305–329.

    Google Scholar 

  9. Adams CE (1972) Aging and reproduction. In: Austin CR, Short RV (eds) Reproduction in mammals: Reproductive patterns, vol. 4. Cambridge University Press, New York, pp 128–156.

    Google Scholar 

  10. Simpkins JW, Mueller GP, Huang HH, Meites J (1977) Evidence for depressed catecholamine and enhanced serotonin metabolism in aging male rats: Possible relation to gonadotropin secretion. Endocrinology 100:1672–1678.

    Article  PubMed  CAS  Google Scholar 

  11. Jeffery S, Carter ND, Clark RG, Robinson ICAF (1990) The episodic secretory pattern of growth hormone regulates liver carbonic anhydrase III. Studies in normal and mutant growth-hormone-deficient dwarf rats. Biochem J 266: 69–74.

    PubMed  CAS  Google Scholar 

  12. Farrell GC, Yoshua DE, Uren RF, Baird PJ, Perkins KW, Kronenberg H (1975) Androgen-induced hepatoma. Lancet I:430–432.

    Article  Google Scholar 

  13. O’Sullivan JP, Rosswick RP (1976) Oral contraceptives and malignant hepatic tumors. Lancet I: 1124–1125.

    Article  Google Scholar 

  14. Nagasue N, Ito A, Yukaya H, Ogawa Y (1985) Androgen receptors in hepatocellular carcinoma and surrounding parenchyma. Gastroenterology 89:643–647.

    PubMed  CAS  Google Scholar 

  15. Nagasue N, Ito A, Yukaya H, Ogawa Y (1986) Estrogen receptors in hepatocellular carcinoma. Cancer 57:87–91.

    Article  PubMed  CAS  Google Scholar 

  16. Yoshida MC, Masuda R, Sasaki M, Takeichi N, Kobayashi H, Dempo K, Mori M (1987) New mutation causing hereditary hepatitis in the laboratory rat. J Hered 78:361–365.

    PubMed  CAS  Google Scholar 

  17. Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) Hereditary hepatitis of LEC rats is controlled by a single autosomal recessive gene. Lab Anim 22:166–169.

    Article  PubMed  CAS  Google Scholar 

  18. Takahashi N, Bauman RA, Ortel TL, Dwulet FE, Wang CC, Putman FW (1983) Internal triplication in the structure of human ceruloplasmin. Proc Natl Acad Sci USA 80:115–119.

    Article  PubMed  CAS  Google Scholar 

  19. Sato M, Schilsky ML, Stockenrt RJ, Morell AG, Sternlieb I (1990) Detection of multiple forms of human ceruloplasmin. A novel Mr 200,000 form. J Biol Chem 265:2533–2537.

    CAS  Google Scholar 

  20. Sugiyama T, Takeichi N, Kobayashi H, Yoshida MC, Sasaki M, Taniguchi N (1988) Metabolic predisposition of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma: Alterations of the drug metabolizing enzymes. Carcinogenesis 9:1569–1572.

    Article  PubMed  CAS  Google Scholar 

  21. Wirth PJ, Benjamin T, Schwartz DM, Thorgeirsson SS (1986) Sequential analysis of chemically induced hepatoma development in rats by two-dimensional electrophoresis. Cancer Res 46:400–413.

    PubMed  CAS  Google Scholar 

  22. Wirth PJ, Fujimoto Y, Takahashi H, Mori M, Yoshida MC, Sugimura T, Nagao M (1990) Two-dimensional electrophoretic analysis of hepatitis-associated polypeptides in liver of LEC rats developing spontaneous hepatitis. Jpn J Cancer Res 81:477–482.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Tokyo

About this chapter

Cite this chapter

Nagase, T. et al. (1991). Identifications of Carbonic Anhydrase III and Triosephosphate Isomerase in the Liver Proteins in LEC Rats on Two-Dimensional Gel Electrophoresis. In: Mori, M., Yoshida, M.C., Takeichi, N., Taniguchi, N. (eds) The LEC Rat. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68153-3_20

Download citation

  • DOI: https://doi.org/10.1007/978-4-431-68153-3_20

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68155-7

  • Online ISBN: 978-4-431-68153-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics