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The LEC Rat pp 175-184 | Cite as

Identifications of Carbonic Anhydrase III and Triosephosphate Isomerase in the Liver Proteins in LEC Rats on Two-Dimensional Gel Electrophoresis

  • Toshihiko Nagase
  • Toshihiro Sugiyama
  • Shigeki Higashiyama
  • Daitoku Sakamuro
  • Sumio Kawata
  • Seiichiro Tarui
  • Naoyuki Taniguchi

Abstract

The two-dimensional gel (2-DG) electrophoresis analysis of total cellular proteins as described by O’Farrell [1] is suitable for investigating certain protein changes which precede hepatitis and/or hepatocellular carcinomas (HCCs).

Keywords

Total Cellular Protein Triosephosphate Isomerase Enlarge Area Internal Amino Acid Sequence Human Ceruloplasmin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021.PubMedGoogle Scholar
  2. 2.
    Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106.PubMedGoogle Scholar
  3. 3.
    Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035–10038.PubMedGoogle Scholar
  4. 4.
    Lu HS, Yuan PM, Gracy RW (1984) Primary structure of human triosephosphate isomerase. J Biol Chem 259:11958–11968.PubMedGoogle Scholar
  5. 5.
    Kelly CD, Carter ND, Jeffery S, Edwards YH (1988) Characterisation of cDNA clones for rat muscle carbonic anhydrase III. Biosci Rep 8:401–406.PubMedCrossRefGoogle Scholar
  6. 6.
    Carter ND, Shiels A, Jeffery S, Heath R, Wilson CA, Phillips IR, Shephard EA (1984) Hormonal control of carbonic anhydrase III. Ann NY Acad Sci 429: 287–301.PubMedCrossRefGoogle Scholar
  7. 7.
    Shiels A, Jeffery S, Phillips IR, Shephard EA, Wilson CA, Carter ND (1983) Sexual differentiation of rat liver carbonic anhydrase III. Biochim Biophys Acta 760:335–342.PubMedCrossRefGoogle Scholar
  8. 8.
    Ramaley JA (1982) The neuroendocrinology of puberty. In: Vernadakis A, Timiras P (eds) Hormones in development and aging. Spectrum, New York pp 305–329.Google Scholar
  9. 9.
    Adams CE (1972) Aging and reproduction. In: Austin CR, Short RV (eds) Reproduction in mammals: Reproductive patterns, vol. 4. Cambridge University Press, New York, pp 128–156.Google Scholar
  10. 10.
    Simpkins JW, Mueller GP, Huang HH, Meites J (1977) Evidence for depressed catecholamine and enhanced serotonin metabolism in aging male rats: Possible relation to gonadotropin secretion. Endocrinology 100:1672–1678.PubMedCrossRefGoogle Scholar
  11. 11.
    Jeffery S, Carter ND, Clark RG, Robinson ICAF (1990) The episodic secretory pattern of growth hormone regulates liver carbonic anhydrase III. Studies in normal and mutant growth-hormone-deficient dwarf rats. Biochem J 266: 69–74.PubMedGoogle Scholar
  12. 12.
    Farrell GC, Yoshua DE, Uren RF, Baird PJ, Perkins KW, Kronenberg H (1975) Androgen-induced hepatoma. Lancet I:430–432.CrossRefGoogle Scholar
  13. 13.
    O’Sullivan JP, Rosswick RP (1976) Oral contraceptives and malignant hepatic tumors. Lancet I: 1124–1125.CrossRefGoogle Scholar
  14. 14.
    Nagasue N, Ito A, Yukaya H, Ogawa Y (1985) Androgen receptors in hepatocellular carcinoma and surrounding parenchyma. Gastroenterology 89:643–647.PubMedGoogle Scholar
  15. 15.
    Nagasue N, Ito A, Yukaya H, Ogawa Y (1986) Estrogen receptors in hepatocellular carcinoma. Cancer 57:87–91.PubMedCrossRefGoogle Scholar
  16. 16.
    Yoshida MC, Masuda R, Sasaki M, Takeichi N, Kobayashi H, Dempo K, Mori M (1987) New mutation causing hereditary hepatitis in the laboratory rat. J Hered 78:361–365.PubMedGoogle Scholar
  17. 17.
    Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) Hereditary hepatitis of LEC rats is controlled by a single autosomal recessive gene. Lab Anim 22:166–169.PubMedCrossRefGoogle Scholar
  18. 18.
    Takahashi N, Bauman RA, Ortel TL, Dwulet FE, Wang CC, Putman FW (1983) Internal triplication in the structure of human ceruloplasmin. Proc Natl Acad Sci USA 80:115–119.PubMedCrossRefGoogle Scholar
  19. 19.
    Sato M, Schilsky ML, Stockenrt RJ, Morell AG, Sternlieb I (1990) Detection of multiple forms of human ceruloplasmin. A novel Mr 200,000 form. J Biol Chem 265:2533–2537.Google Scholar
  20. 20.
    Sugiyama T, Takeichi N, Kobayashi H, Yoshida MC, Sasaki M, Taniguchi N (1988) Metabolic predisposition of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma: Alterations of the drug metabolizing enzymes. Carcinogenesis 9:1569–1572.PubMedCrossRefGoogle Scholar
  21. 21.
    Wirth PJ, Benjamin T, Schwartz DM, Thorgeirsson SS (1986) Sequential analysis of chemically induced hepatoma development in rats by two-dimensional electrophoresis. Cancer Res 46:400–413.PubMedGoogle Scholar
  22. 22.
    Wirth PJ, Fujimoto Y, Takahashi H, Mori M, Yoshida MC, Sugimura T, Nagao M (1990) Two-dimensional electrophoretic analysis of hepatitis-associated polypeptides in liver of LEC rats developing spontaneous hepatitis. Jpn J Cancer Res 81:477–482.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Toshihiko Nagase
    • 1
    • 2
  • Toshihiro Sugiyama
    • 1
  • Shigeki Higashiyama
    • 1
  • Daitoku Sakamuro
    • 1
  • Sumio Kawata
    • 2
  • Seiichiro Tarui
    • 2
  • Naoyuki Taniguchi
    • 1
  1. 1.Department of BiochemistryOsaka University Medical SchoolSuita, Osaka, 565Japan
  2. 2.Second Department of Internal MedicineOsaka University Medical SchoolSuita, Osaka, 565Japan

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