Abstract
The two-dimensional gel (2-DG) electrophoresis analysis of total cellular proteins as described by O’Farrell [1] is suitable for investigating certain protein changes which precede hepatitis and/or hepatocellular carcinomas (HCCs).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021.
Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106.
Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035–10038.
Lu HS, Yuan PM, Gracy RW (1984) Primary structure of human triosephosphate isomerase. J Biol Chem 259:11958–11968.
Kelly CD, Carter ND, Jeffery S, Edwards YH (1988) Characterisation of cDNA clones for rat muscle carbonic anhydrase III. Biosci Rep 8:401–406.
Carter ND, Shiels A, Jeffery S, Heath R, Wilson CA, Phillips IR, Shephard EA (1984) Hormonal control of carbonic anhydrase III. Ann NY Acad Sci 429: 287–301.
Shiels A, Jeffery S, Phillips IR, Shephard EA, Wilson CA, Carter ND (1983) Sexual differentiation of rat liver carbonic anhydrase III. Biochim Biophys Acta 760:335–342.
Ramaley JA (1982) The neuroendocrinology of puberty. In: Vernadakis A, Timiras P (eds) Hormones in development and aging. Spectrum, New York pp 305–329.
Adams CE (1972) Aging and reproduction. In: Austin CR, Short RV (eds) Reproduction in mammals: Reproductive patterns, vol. 4. Cambridge University Press, New York, pp 128–156.
Simpkins JW, Mueller GP, Huang HH, Meites J (1977) Evidence for depressed catecholamine and enhanced serotonin metabolism in aging male rats: Possible relation to gonadotropin secretion. Endocrinology 100:1672–1678.
Jeffery S, Carter ND, Clark RG, Robinson ICAF (1990) The episodic secretory pattern of growth hormone regulates liver carbonic anhydrase III. Studies in normal and mutant growth-hormone-deficient dwarf rats. Biochem J 266: 69–74.
Farrell GC, Yoshua DE, Uren RF, Baird PJ, Perkins KW, Kronenberg H (1975) Androgen-induced hepatoma. Lancet I:430–432.
O’Sullivan JP, Rosswick RP (1976) Oral contraceptives and malignant hepatic tumors. Lancet I: 1124–1125.
Nagasue N, Ito A, Yukaya H, Ogawa Y (1985) Androgen receptors in hepatocellular carcinoma and surrounding parenchyma. Gastroenterology 89:643–647.
Nagasue N, Ito A, Yukaya H, Ogawa Y (1986) Estrogen receptors in hepatocellular carcinoma. Cancer 57:87–91.
Yoshida MC, Masuda R, Sasaki M, Takeichi N, Kobayashi H, Dempo K, Mori M (1987) New mutation causing hereditary hepatitis in the laboratory rat. J Hered 78:361–365.
Masuda R, Yoshida MC, Sasaki M, Dempo K, Mori M (1988) Hereditary hepatitis of LEC rats is controlled by a single autosomal recessive gene. Lab Anim 22:166–169.
Takahashi N, Bauman RA, Ortel TL, Dwulet FE, Wang CC, Putman FW (1983) Internal triplication in the structure of human ceruloplasmin. Proc Natl Acad Sci USA 80:115–119.
Sato M, Schilsky ML, Stockenrt RJ, Morell AG, Sternlieb I (1990) Detection of multiple forms of human ceruloplasmin. A novel Mr 200,000 form. J Biol Chem 265:2533–2537.
Sugiyama T, Takeichi N, Kobayashi H, Yoshida MC, Sasaki M, Taniguchi N (1988) Metabolic predisposition of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma: Alterations of the drug metabolizing enzymes. Carcinogenesis 9:1569–1572.
Wirth PJ, Benjamin T, Schwartz DM, Thorgeirsson SS (1986) Sequential analysis of chemically induced hepatoma development in rats by two-dimensional electrophoresis. Cancer Res 46:400–413.
Wirth PJ, Fujimoto Y, Takahashi H, Mori M, Yoshida MC, Sugimura T, Nagao M (1990) Two-dimensional electrophoretic analysis of hepatitis-associated polypeptides in liver of LEC rats developing spontaneous hepatitis. Jpn J Cancer Res 81:477–482.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Tokyo
About this chapter
Cite this chapter
Nagase, T. et al. (1991). Identifications of Carbonic Anhydrase III and Triosephosphate Isomerase in the Liver Proteins in LEC Rats on Two-Dimensional Gel Electrophoresis. In: Mori, M., Yoshida, M.C., Takeichi, N., Taniguchi, N. (eds) The LEC Rat. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68153-3_20
Download citation
DOI: https://doi.org/10.1007/978-4-431-68153-3_20
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68155-7
Online ISBN: 978-4-431-68153-3
eBook Packages: Springer Book Archive