Our previous studies indicated that LEC rats are characterized by a high sensitivity to azacytidine , which is a well-known inhibitor of DNA-methyltransferase. In addition, control rats fed choline- and methioninedeficient diets mimic LEC rats, suggesting that the LEC rat has some direct defect in DNA methylation or that the defect is accompanied by a hypomethylated state . Choline deficiency has been reported to induce accumulation of fats in the liver and reduction in circulating phospholipids, triglycerides, and cholesterol in plasma [3–5]. The development of fatty liver in choline-deficient rats could be a result of increased lipid biosynthesis, inhibition of lipid degradation, fatty acid oxidation, or a failure to transfer the newly synthesized triglyceride into the plasma with a resultant increase in liver triglyceride content .
KeywordsCholesterol Ester Free Fatty Acid Level Choline Deficiency Abnormal Lipid Metabolism Hypomethylated State
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- 1.Suzuki K, Sugiyama T, Ookawara T, Kurosawa T, Taniguchi N (to be published) High sensitivity to 5-azacytidine in LEC rats, a strain with a metabolic predisposition to hepatitis and hepatoma: Possible involvement of DNA methylation in the expression of cytochrome P-450 and γ-glutamyl transpeptidase. Biochem Int.Google Scholar
- 2.Sugiyama T, Matsunaga M, Jain SK, Jain S, Ikeda Y, Taniguchi N (to be published) Enhancing effect of a choline-deficient diet on alterations of hepatic drug-metabolizing enzymes in hepatitis-and hepatoma-predisposed rats (LEC rats) Jap J Cancer Res.Google Scholar