Abstract
Although the pathological changes in the liver of LEC rats after the onset of hepatitis are well established, the natural history of LEC rats before the manifestation of jaundice remains unresolved. Before the onset of hepatitis, the LEC rat is normal, at least as far as liver function tests are concerned. However, there are some reports that describe the presence of pathological changes in the liver of LEC rats before the onset of hepatitis, i.e., the appearance of abnormally high ploidy nuclei [1] and nuclear enlargement [2] in hepatocytes. Since these findings from different individual rats tend to be somewhat fragmentary, it seems worthy to assess whether such pathological findings are observed consistently in the liver of the same LEC rat throughout its life course before the onset of hepatitis. Therefore in this study, we designed a prospective follow-up study of the liver of LEC rats using consecutive needle biopsies. Much attention was placed on the ultrastructural changes in the nuclei in light of the abnormalities in the ploidy and nuclear size which have previously been reported.
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References
Fujimoto Y, Oyamada M, Hattori A, Takahashi H, Satoh M, Dempo K, Mori M, Masuda R (1988) DNA analysis of megalocytic hepatocytes in LEC rats. Jpn Acta Hepatol 29:967–968.
Dempo K, Oyamada M, Fujimoto Y, Takahashi H, Satoh M, Hattori A, Mori M (1988) Pathological changes of the liver in LEC rats with hereditary hepatitis. J Toxicol Pathol 1:53–60.
Sakamoto H, Kamimura Y, Takahashi H, Nagai H, Yamaguchi T, Inaoka K, Minase T, Mori M (to be published) Sequential ultrastructural observations of the liver of LEC rats before the onset of hereditary hepatitis. J Clin Electron Microscopy 23.
Zimmerman HJ (1978) Hepatotoxicity. In: Lesions seen by electron microscopy. Appleton-Century-Crofts, New York pp 80–84.
De Man JCH, Noorduyn NJ (1967) Light and electron microscopic radioautography of hepatic cell nucleoli in mice treated with actinomycin D. J Cell Biol 33:489–496.
Merski JA, Daskal I, Busch H (1976) Effects of adriamycin on ultrastructure of nucleoli in the heart and liver cells of rat. Cancer Res 36:1580–1584.
Lantos PL (1971) The effect of a single dose of N-ethyl-N-nitrosourea in the fine structure of the brain of the rat. Experientia 27:1322–1321.
Sirtori C, Bosisio M (1966) Oncolysis by Herpes simplex. Lancet I:96.
Shimizu K, Abe M, Yokoyama S, Takahashi H, Sawada N, Mori M, Tsukada K (1990) Decreased activities of S-adenosylmethionine synthetase isozymes in hereditary hepatitis in Long-Evans rats. Life Sci 46:1837–1840.
Dimova RN, Gajdardjieva KC, Dabeva MD, Hadjiolov AA (1979) Early effects of D-galactosamine on rat liver nucleolar structures. Biol Cell 35:1–10.
Clawson GA, Sesno J, Milam K, Wang Y-F, Gabriel C (1990) The hepatocyte protein synthesis defect induced by galactosamine involves hypomethylation of ribosomal RNA. Hepatology 11:428–434.
Howat AJ, Cotton DWK, Slater DN (1989) Nucleolar organizer regions in spitz nevi and malignant melanomas. Cancer 63:474–478.
Minase T, Piao Zhe-Si, Takahashi H, Hattori A, Mori M, Yokoyama S (1989) Ultrastructural changes of the liver in LEC rats with hereditary hepatitis. J Clin Electron Microscopy 22:783–784.
Schaffner F, Popper H (1975) Electron microscopy in the liver. In: Shiff L (ed) Diseases of the liver, 4th edn. Lippincott, Philadelphia, pp 51–86.
Svoboda D, Higginson J (1968) A comparison of ultrastructural changes in rat liver due to chemical carcinogens. Cancer Res 28:1703–1733.
Li Y, Togashi Y, Sato S, Emoto T, Kang J-H, Takeichi N, Kobayashi H, Kojima Y, Une Y, Uchino J (to be published) Spontaneous hepatic copper accumulation in LEC rats with hereditary hepatitis: A model of Wilson’s disease. J Clin Invest.
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© 1991 Springer-Verlag Tokyo
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Sakamoto, H., Kamimura, Y., Minase, T., Yokoyama, S., Satoh, M., Mori, M. (1991). Consecutive Follow-up Study of the Liver of LEC Rats Before the Onset of Spontaneous Hepatitis. In: Mori, M., Yoshida, M.C., Takeichi, N., Taniguchi, N. (eds) The LEC Rat. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68153-3_10
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DOI: https://doi.org/10.1007/978-4-431-68153-3_10
Publisher Name: Springer, Tokyo
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