The LEC Rat pp 95-102 | Cite as

Consecutive Follow-up Study of the Liver of LEC Rats Before the Onset of Spontaneous Hepatitis

  • Hirofumi Sakamoto
  • Yasuhiro Kamimura
  • Takashi Minase
  • Shigeaki Yokoyama
  • Masaaki Satoh
  • Michio Mori


Although the pathological changes in the liver of LEC rats after the onset of hepatitis are well established, the natural history of LEC rats before the manifestation of jaundice remains unresolved. Before the onset of hepatitis, the LEC rat is normal, at least as far as liver function tests are concerned. However, there are some reports that describe the presence of pathological changes in the liver of LEC rats before the onset of hepatitis, i.e., the appearance of abnormally high ploidy nuclei [1] and nuclear enlargement [2] in hepatocytes. Since these findings from different individual rats tend to be somewhat fragmentary, it seems worthy to assess whether such pathological findings are observed consistently in the liver of the same LEC rat throughout its life course before the onset of hepatitis. Therefore in this study, we designed a prospective follow-up study of the liver of LEC rats using consecutive needle biopsies. Much attention was placed on the ultrastructural changes in the nuclei in light of the abnormalities in the ploidy and nuclear size which have previously been reported.


Nucleolar Organizer Region Smooth Endoplasmic Reticulum Bile Canaliculus Liver Biopsy Specimen Granular Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Fujimoto Y, Oyamada M, Hattori A, Takahashi H, Satoh M, Dempo K, Mori M, Masuda R (1988) DNA analysis of megalocytic hepatocytes in LEC rats. Jpn Acta Hepatol 29:967–968.CrossRefGoogle Scholar
  2. 2.
    Dempo K, Oyamada M, Fujimoto Y, Takahashi H, Satoh M, Hattori A, Mori M (1988) Pathological changes of the liver in LEC rats with hereditary hepatitis. J Toxicol Pathol 1:53–60.Google Scholar
  3. 3.
    Sakamoto H, Kamimura Y, Takahashi H, Nagai H, Yamaguchi T, Inaoka K, Minase T, Mori M (to be published) Sequential ultrastructural observations of the liver of LEC rats before the onset of hereditary hepatitis. J Clin Electron Microscopy 23.Google Scholar
  4. 4.
    Zimmerman HJ (1978) Hepatotoxicity. In: Lesions seen by electron microscopy. Appleton-Century-Crofts, New York pp 80–84.Google Scholar
  5. 5.
    De Man JCH, Noorduyn NJ (1967) Light and electron microscopic radioautography of hepatic cell nucleoli in mice treated with actinomycin D. J Cell Biol 33:489–496.PubMedCrossRefGoogle Scholar
  6. 6.
    Merski JA, Daskal I, Busch H (1976) Effects of adriamycin on ultrastructure of nucleoli in the heart and liver cells of rat. Cancer Res 36:1580–1584.PubMedGoogle Scholar
  7. 7.
    Lantos PL (1971) The effect of a single dose of N-ethyl-N-nitrosourea in the fine structure of the brain of the rat. Experientia 27:1322–1321.PubMedCrossRefGoogle Scholar
  8. 8.
    Sirtori C, Bosisio M (1966) Oncolysis by Herpes simplex. Lancet I:96.CrossRefGoogle Scholar
  9. 9.
    Shimizu K, Abe M, Yokoyama S, Takahashi H, Sawada N, Mori M, Tsukada K (1990) Decreased activities of S-adenosylmethionine synthetase isozymes in hereditary hepatitis in Long-Evans rats. Life Sci 46:1837–1840.PubMedCrossRefGoogle Scholar
  10. 10.
    Dimova RN, Gajdardjieva KC, Dabeva MD, Hadjiolov AA (1979) Early effects of D-galactosamine on rat liver nucleolar structures. Biol Cell 35:1–10.Google Scholar
  11. 11.
    Clawson GA, Sesno J, Milam K, Wang Y-F, Gabriel C (1990) The hepatocyte protein synthesis defect induced by galactosamine involves hypomethylation of ribosomal RNA. Hepatology 11:428–434.PubMedCrossRefGoogle Scholar
  12. 12.
    Howat AJ, Cotton DWK, Slater DN (1989) Nucleolar organizer regions in spitz nevi and malignant melanomas. Cancer 63:474–478.PubMedCrossRefGoogle Scholar
  13. 13.
    Minase T, Piao Zhe-Si, Takahashi H, Hattori A, Mori M, Yokoyama S (1989) Ultrastructural changes of the liver in LEC rats with hereditary hepatitis. J Clin Electron Microscopy 22:783–784.Google Scholar
  14. 14.
    Schaffner F, Popper H (1975) Electron microscopy in the liver. In: Shiff L (ed) Diseases of the liver, 4th edn. Lippincott, Philadelphia, pp 51–86.Google Scholar
  15. 15.
    Svoboda D, Higginson J (1968) A comparison of ultrastructural changes in rat liver due to chemical carcinogens. Cancer Res 28:1703–1733.PubMedGoogle Scholar
  16. 16.
    Li Y, Togashi Y, Sato S, Emoto T, Kang J-H, Takeichi N, Kobayashi H, Kojima Y, Une Y, Uchino J (to be published) Spontaneous hepatic copper accumulation in LEC rats with hereditary hepatitis: A model of Wilson’s disease. J Clin Invest.Google Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Hirofumi Sakamoto
  • Yasuhiro Kamimura
  • Takashi Minase
  • Shigeaki Yokoyama
  • Masaaki Satoh
  • Michio Mori
    • 1
  1. 1.Department of PathologySapporo Medical CollegeSapporo, 060Japan

Personalised recommendations