Double-Labeling Method with BUdR and IUdR for Cell Kinetic Studies of Brain Tumors
Bromodeoxyuridine (BUdR) and iododeoxyuridine (IUdR) are known to be incorporated into cellular DNA during DNA synthesis, and can be recognized by immunohistochemical or immunofluorescent techniques utilizing anti-BUdR or anti-IUdR monoclonal antibodies [1–4]. Recently, two new monoclonal antibodies were developed by Vanderlaan et al.: Br-3, which recognizes only BUdR, and IU-4, which recognizes both BUdR and IUdR. By administering IUdR and BUdR at different time sequences, it is possible to determine not only the S-phase fraction but also to measure the rate of cell cycle progression and thus calculate the duration of S-phase, the cell cycle time or growth fraction of an individual tumor from a single tumor biopsy. This report describes double-labeling and staining techniques for estimating the duration of S-phase and the doubling time of biopsied materials.
KeywordsBlack Reaction Cell Cycle Time Thymidine Label Index Cell Kinetic Study Potential Doubling Time
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- 3.Gray JW, Mayall BH (1986) Monoclonal antibodies against bromodeoxyuridine. Alan R. Liss, New YorkGoogle Scholar
- 5.Dolbeare F, Kuo WL, Vanderlaan M, Gray JW (1988) Cell cycle analysis by flow cytometric analysis of the incorporation of iododeoxyuridine (IdUrd) and bro-modeoxyuridine ( BrdUrd ). Proc Am Assoc Cancer Res 29: 1896Google Scholar
- 7.Sasaki K (1977) Measurement of tritiated thymidine labeling index by incubation in vitro of surgically removed cervical cancer. Jpn J Cancer Research 68: 307–313Google Scholar
- 10.Hoshino T, Barker M, Wilson CB, Boldrey EB, Fewer, D (1968) Cell kinetics of human gliomas. J Neurosurg 37: 15–26Google Scholar
- 11.Steel GG (1968) Cell loss from experimental tumors. Cell Tissue Kinet 1: 193 - 207Google Scholar