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Double-Labeling Method with BUdR and IUdR for Cell Kinetic Studies of Brain Tumors

  • Soichiro Shibui
  • Ryo Nishikawa
  • Kazuhiro Nomura
  • Keiko Iwasaki
  • Tatsuhiro Maeda
  • Takao Hoshino

Abstract

Bromodeoxyuridine (BUdR) and iododeoxyuridine (IUdR) are known to be incorporated into cellular DNA during DNA synthesis, and can be recognized by immunohistochemical or immunofluorescent techniques utilizing anti-BUdR or anti-IUdR monoclonal antibodies [1–4]. Recently, two new monoclonal antibodies were developed by Vanderlaan et al.: Br-3[5], which recognizes only BUdR, and IU-4[6], which recognizes both BUdR and IUdR. By administering IUdR and BUdR at different time sequences, it is possible to determine not only the S-phase fraction but also to measure the rate of cell cycle progression and thus calculate the duration of S-phase, the cell cycle time or growth fraction of an individual tumor from a single tumor biopsy. This report describes double-labeling and staining techniques for estimating the duration of S-phase and the doubling time of biopsied materials.

Keywords

Black Reaction Cell Cycle Time Thymidine Label Index Cell Kinetic Study Potential Doubling Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Soichiro Shibui
    • 1
  • Ryo Nishikawa
    • 1
  • Kazuhiro Nomura
    • 1
  • Keiko Iwasaki
    • 2
  • Tatsuhiro Maeda
    • 3
  • Takao Hoshino
    • 3
  1. 1.Department of NeurosurgeryNational Cancer CenterChuo-Ku, Tokyo, 104Japan
  2. 2.FCM Analysis RoomNational Cancer CenterChuo-Ku, Tokyo, 104Japan
  3. 3.Department of NeurosurgeryKyorin University School of MedicineMitaka, Tokyo, 181Japan

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