Abstract
Bromodeoxyuridine (BUdR) and iododeoxyuridine (IUdR) are known to be incorporated into cellular DNA during DNA synthesis, and can be recognized by immunohistochemical or immunofluorescent techniques utilizing anti-BUdR or anti-IUdR monoclonal antibodies [1–4]. Recently, two new monoclonal antibodies were developed by Vanderlaan et al.: Br-3[5], which recognizes only BUdR, and IU-4[6], which recognizes both BUdR and IUdR. By administering IUdR and BUdR at different time sequences, it is possible to determine not only the S-phase fraction but also to measure the rate of cell cycle progression and thus calculate the duration of S-phase, the cell cycle time or growth fraction of an individual tumor from a single tumor biopsy. This report describes double-labeling and staining techniques for estimating the duration of S-phase and the doubling time of biopsied materials.
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© 1991 Springer-Verlag Tokyo
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Shibui, S., Nishikawa, R., Nomura, K., Iwasaki, K., Maeda, T., Hoshino, T. (1991). Double-Labeling Method with BUdR and IUdR for Cell Kinetic Studies of Brain Tumors. In: Tabuchi, K. (eds) Biological Aspects of Brain Tumors. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68150-2_9
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DOI: https://doi.org/10.1007/978-4-431-68150-2_9
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68152-6
Online ISBN: 978-4-431-68150-2
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