Differentiation of a Medulloblastoma Cell Line Mediated by Protein Kinase A

  • Takashi Kokunai
  • Norihiko Tamaki
  • Satoshi Matsumoto


Cyclic AMP has been implicated in the regulation of the growth and differentiation of various normal and malignant cells [1,2]. In neuroblastoma and glioma cells, it has been shown that N6,O2′-dibutyryl cyclic AMP (Bt2-cAMP) induces morphological and biochemical differentiation in vitro [3]. It has been proposed that most, if not all, of the effects of cyclic AMP are mediated by cyclic AMP-dependent protein kinase (protein kinase A). It was also reported that the modulators of protein kinase C system might be induced by differentiation in some neuroblastoma and glioma cells [4,5].


Glioma Cell Neuroblastoma Cell Medulloblastoma Cell Human Medulloblastoma Cell Line cAMP Binding Protein 


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  1. 1.
    Cohen P (1982) The role of protein phosphorylation in neural and hormonal control of cellular activity. Nature 296: 613–620PubMedCrossRefGoogle Scholar
  2. 2.
    Friedman DL (1976) Role of cyclic nucleotides in cell growth and differentiation. Physiol Rev 56: 652–707PubMedGoogle Scholar
  3. 3.
    Prashad N, Lotan D, Lotan R (1987) Differential effect of dibutyryl cyclic adenosine monophosphate and retinoic acid on the growth, differentiation and cyclic adenosine monophosphate-binding protein on murine neuroblastoma cells. Cancer Res 47: 2417–2424PubMedGoogle Scholar
  4. 4.
    Hama T, Huang KP, Guroff G (1986) Protein kinase C as a component of a nerve growth factor-sensitive phosphorylation system in PC 12 cells. Proc Natl Acad Sci USA 83: 2353–2357PubMedCrossRefGoogle Scholar
  5. 5.
    Couldwell WT, Antel JP, Apuzzo ML J, Young VW (1990) Inhibition of growth of established human glioma cell lines by modulators of the protein kinase C system. J Neurosurg 73: 594–600PubMedCrossRefGoogle Scholar
  6. 6.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685PubMedCrossRefGoogle Scholar
  7. 7.
    Prashad N, Rosenberg RN (1978) Induction of cAMP binding protein by dibutyryl cAMP in mouse neuroblastoma cell. Biochim Biophys Acta 539: 459–469PubMedCrossRefGoogle Scholar
  8. 8.
    Greengard P (1978) Phospholylated proteins as physiological effectors. Science 199: 146–152PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Takashi Kokunai
  • Norihiko Tamaki
  • Satoshi Matsumoto
    • 1
  1. 1.Department of NeurosurgeryKobe University School of MedicineKobe, 650Japan

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