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A Molecular Basis for Multidrug-Resistance and Reversal of the Resistance

  • Shin-Ichi Akiyama
  • Akihiko Yoshimura
  • Misako Ichikawa
  • Tomoyuki Sumizawa
  • Tatsuhiko Furukawa

Abstract

The development of multidrug-resistance (MDR) is a serious problem during treatment of various malignant tumors. Multidrug-resistant cells were isolated from human carcinoma cell lines and studied to elucidate the molecular basis for their multidrug resistance properties [1–4].

Keywords

Multidrug Resistance Acute Myelogenous Leukemia Photoaffinity Label Human Carcinoma Cell Line Reverse Drug Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Biedler JL, Riehm H (1970) Cellular resistance to actinomycin D in Chinese hamster cells in vitro: Cross-resistance, radioautographic, and cytogenetic studies. Cancer Res 30: 1174–1184PubMedGoogle Scholar
  2. 2.
    Ling V, Thompson LH (1973) Reduced permeability in CHO cells as a mechanism of resistance to colchicine. J Cell Physiol 83: 103–116CrossRefGoogle Scholar
  3. 3.
    Beck WT, Mueller TJ, Tanzer LR (1979) Altered surface membrane glycoproteins in Vinca alkaloid-resistant human leukemic lymphoblasts. Cancer Res 39: 2070–2076PubMedGoogle Scholar
  4. 4.
    Akiyama S, Fojo A, Hanover JA, Pastan I, Gottesman MM (1985) Isolation and genetic characterization of human KB cell lines resistant to multiple drugs. Somatic Cell Mol Genet 11: 117–126CrossRefGoogle Scholar
  5. 5.
    Juliano RL, Ling V (1976) A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta 455: 152–162PubMedCrossRefGoogle Scholar
  6. 6.
    Kartner, N, Evernden-Porelle D, Bradley G, Ling V (1985) Detection of P-glyco- protein in multidrug-resistant cell lines by monoclonal antibodies. Nature 316: 820–823PubMedCrossRefGoogle Scholar
  7. 7.
    Kartner N, Riordan JR, Ling V (1983) Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines. Science 221: 1285–1288PubMedCrossRefGoogle Scholar
  8. 8.
    Shen DW, Cardarelli C, Hwang J, Cornwell MM, Richert N, Ishii S, Pastan I, Gottesman MM (1986) Multiple drug resistant human KB carcinoma cells in-dependently selected for high-level resistance to colchicine, Adriamycin, or vinblastine show changes in expression of specific proteins. J Biol Chem 261: 7762–7770PubMedGoogle Scholar
  9. 9.
    Chen CJ, Chin JE, Ueda K, Clark DP, Pastan I, Gottesman MM, Roninson IB (1986) Internal duplication and homology with bacterial transport proteins in the mdrl ( P-glycoprotein) gene from multidrug-resistant human cells. Cell 47: 381–389PubMedCrossRefGoogle Scholar
  10. 10.
    Gros P, Croop J, Housman D (1986) Mammalian multidrug resistance gene: Complete cDNA sequence indicates strong homology to bacterial transport protein. Cell 47: 371–380PubMedCrossRefGoogle Scholar
  11. 11.
    Gerlach JH, Endicot A, Juranka R, Henderson G, Sarangi F, Deuchars KL, Ling V (1986) Homology between P-glycoprotein and a bacterial haemolysin transport protein suggests a model for multidrug resistance. Nature 324: 485–489PubMedCrossRefGoogle Scholar
  12. 12.
    Cornwell MM, Safa AR, Felsted RL, Gottesman MM, Pastan I (1986) Membrane vesicles from multidrug-resistant human cancer cells contain a specific 150 to 170 kDa protein detected by photoaffinity labeling. Proc Natl Acad Sci USA 83: 3847–3850PubMedCrossRefGoogle Scholar
  13. 13.
    Safa AR, Glover CJ, Meyers MB, Biedler JL, Felsted RL (1986) Vinblastine photoaffinity labeling of a high molecular weight surface membrane glycoprotein specific for multidrug-resistant cells. J Biol Chem 26: 6137–6140Google Scholar
  14. 14.
    Akiyama S, Cornwell MM, Kuwano M, Pastan I, Gottesman MM (1988) Most drugs that reverse multidrug resistance also inhibit photoaffinity labeling of P- glycoprotein by a vinblastine analog. Mol Pharmacol 33: 144–147PubMedGoogle Scholar
  15. 15.
    Safa AR, Glover CJ, Sewell JL, Meyers MB, Biedler JL, Felsted RL (1987) Identification of the multidrug resistance-related membrane glycoprotein as an acceptor for calcium channel blockers. J Biol Chem 262: 7884–7888PubMedGoogle Scholar
  16. 16.
    Safa AR (1988) Photoaffinity labeling of the multidrug-resistance related P- glycoprotein with photoactive analogs of verapamil. Proc Natl Acad Sci USA 85: 7187–7191PubMedCrossRefGoogle Scholar
  17. 17.
    Akiyama S, Yoshimura A, Kikuchi H, Sumizawa, T, Kuwano M, Tahara Y (1989) Synthetic isoprenoid photoaffinity labeling of P-glycoprotein specific to multidrug- resistant cells. Mol Pharmacol 36: 730–735PubMedGoogle Scholar
  18. 18.
    Cornwell MM, Tsuruo T, Gottesman MM, Pastan I (1987) ATP-binding properties of P-glycoprotein from multidrug-resistant KB cells. FASEB J 1: 51–54PubMedGoogle Scholar
  19. 19.
    Gottesman MM, Pastan I (1988) The multidrug transporter, a double-edged sword. J Biol Chem 263: 12163 - 12166PubMedGoogle Scholar
  20. 20.
    Kuwazuru Y, Yoshimura A, Hanada S, Ichikawa M, Saito T, Uozumi K, Utsuno- miya A, Arima T, Akiyama S (1990) Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukemia cells in blast crisis. Brit J Haematol 74: 24–29CrossRefGoogle Scholar
  21. 21.
    Kuwazuru Y, Yoshimura A, Hanada S, Makino T, Terada A, Utsunomiya A, Arima T, Akiyama S (1990) Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance. Cancer 66: 868–873PubMedCrossRefGoogle Scholar
  22. 22.
    Kuwazuru Y, Hanada S, Furukawa T, Yoshimura A, Sumizawa T, Utsunomiya A, Ishibashi K, Saito T, Arima T, Akiyama S (1990) Expression of P-glycoprotein in adult T cell leukemia cells. Blood 76: 2065–2071PubMedGoogle Scholar
  23. 23.
    Mizoguchi T, Yamada K, Furukawa T, Hidaka K, Hisatsugu T, Shimazu H, Tsuruo T, Sumizawa T, Akiyama S (1990) Expression of the MDR1 gene in human gastric and colorectal carcinomas. J Natl Cancer Inst 82: 1679–1683PubMedCrossRefGoogle Scholar
  24. 24.
    Kamiwatari M, Nagata Y, Kikuchi H, Yoshimura A, Sumizawa T, Shudo N, Sakoda R, Seto K, Akiyama S (1989) Correlation between reversing of multidrug resistance and inhibiting to [3H]azidopine photolabeling of P-glycoprotein by newly synthesized dihydropyridine analogues in a human cell line. Cancer Res 49: 3190–3195PubMedGoogle Scholar
  25. 25.
    Shudo N, Mizoguchi T, Kiyosue T, Arita M, Yoshimura A, Seto K, Sakoda R, Akiyama S (1990) Two pyridine analogues with more effective ability to reverseGoogle Scholar
  26. 26.
    Cornwell MM, Safa AR, Felsted RL, Gottesman MM, Pastan I (1986) Membrane vesicles from multidrug resistant human cancer cells contain a specific 150 to 170 kDa protein detected by photoaffinity labeling. Proc Natl Acad Sci USA 83: 3847–3850PubMedCrossRefGoogle Scholar
  27. 27.
    Safa AR, Glover CJ, Sewell JL, Meyers MB, Biedler JL, Felsted RL (1987) Identification of the multidrug resistance-related membrane glycoprotein as an acceptor for calcium channel blockers. J Biol Chem 262: 7884–7888PubMedGoogle Scholar
  28. 28.
    Yoshimura A, Kuwazuru Y, Sumizawa T, Ichikawa M, Ikeda S, Uda T, Akiyama S (1989) Cytoplasmic orientation and two-domain structure of the multidrug transporter, P-glycoprotein, demonstrated with sequence-specific antibodies. J Biol Chem 264: 16282–16291PubMedGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Shin-Ichi Akiyama
  • Akihiko Yoshimura
  • Misako Ichikawa
  • Tomoyuki Sumizawa
  • Tatsuhiko Furukawa
    • 1
  1. 1.Department of Cancer Chemotherapy, Institute of Cancer Research, Faculty of MedicineKagoshima UniversityKagoshima, 890Japan

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