Advertisement

Opposite Effects of Cyclic AMP and Cell Density on Expression of αB-Crystallin and Glial Fibrillary Acidic Protein in C-6 Glioma Cells

  • Akiko Iwaki
  • Toru Iwaki
  • Yoshiyuki Sakaki
  • Ronald K. H. Liem
  • James E. Goldman

Abstract

Crystallins are well known to be water-soluble proteins in the lens that can pack together efficiently to form very large aggregates. α-Crystallin is a heterogeneous aggregate produced by the products of two genes, αA and αB. Of these two genes, αA-crystallin expression seems restricted to the lens [1], In contrast, αB-crystallin is expressed in various extra-ocular tissues such as heart, muscle, kidney, brain, nerve, and placenta [2,3]. The function of αB-crystallin in extra-ocular tissues is not yet known.

Keywords

Glial Fibrillary Acidic Protein Rosenthal Fiber Involve Protein Kinase Glial Fibrillary Acidic Protein mRNA Glial Fibrillary Acidic Protein Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dubin RA, Wawrousek EF, Piategorsky J (1989) Expression of the murine αB-crystallin gene is not restricted to the lens. Mol Cell Biol 9: 1083–1091PubMedGoogle Scholar
  2. 2.
    Iwaki T, Kume-Iwaki A, Liem RKH, Goldman JE (1989) αB-Crystallin is expressed in non-lenticular tissues and accumulates in Alexander’s disease brain. Cell 57: 71–78PubMedCrossRefGoogle Scholar
  3. 3.
    Iwaki T, Kume-Iwaki A, Goldman JE (1990) Cellular distribution of αB-crystallin in non-lenticular tissues. J Histochem Cytochem 38: 31–39PubMedCrossRefGoogle Scholar
  4. 4.
    Goldman JE, Corbin E (1988) Isolation of a major protein component of Rosenthal fibers. Am J Pathol 130: 569–578PubMedGoogle Scholar
  5. 5.
    Iwaki T, Kume-Iwaki A, Corbin E, Goldman JE (1990) Expression of the B-chain of α-crystallin in CNS glia (abstract). J Neuropathol Exp Neurol 49: 344CrossRefGoogle Scholar
  6. 6.
    Shafit-Zagardo B, Kume-Iwaki A, Goldman JE (1988) Astrocytes regulate GFAP mRNA levels by cyclic AMP and protein kinase C-dependent mechanisms. Glia 1: 346–354PubMedCrossRefGoogle Scholar
  7. 7.
    Duguid JR, Rohwer RG, Seed B (1988) Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library. Proc Natl Acad Sci USA 85: 5738–5742PubMedCrossRefGoogle Scholar
  8. 8.
    Benda P, Lightbody J, Sato G, Levine L, Sweet W (1968) Differential rat glial cell strain in tissue culture. Science 161: 370–371PubMedCrossRefGoogle Scholar
  9. 9.
    Goldman JE, Chiu F-C (1984) Dibutyryl cyclic AMP causes intermediate filament accumulation and actin reorganization in astrocytes. Brain Res 306: 85–95PubMedCrossRefGoogle Scholar
  10. 10.
    Iwaki A, Iwaki T, Goldman JE, Liem RKH (1990) Multiple mRNAs of rat brain α-crystallin B chain result from alternative transcriptional initiation. J Biol Chem 265: 22197–22203PubMedGoogle Scholar
  11. 11.
    Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308: 693–698PubMedCrossRefGoogle Scholar
  12. 12.
    Miura M, Tamura T, Mikoshiba, K (1990) Cell-specific expression of the mouse glial fibrillary acidic protein gene: Identification of the cis- and trans-acting promoter elements for astrocyte-specific expression. J Neurochem 55: 1180–1188PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Akiko Iwaki
    • 1
  • Toru Iwaki
    • 2
  • Yoshiyuki Sakaki
    • 1
  • Ronald K. H. Liem
    • 3
  • James E. Goldman
    • 3
  1. 1.Research Laboratory for Genetic InformationKyushu University 60Fukuoka, 812Japan
  2. 2.Department of Neuropathology, Neurological Institute, Faculty of MedicineKyushu University 60Fukuoka, 812Japan
  3. 3.Department of PathologyColumbia University College of Physicians and SurgeonsNew YorkUSA

Personalised recommendations