The Influence of MHC Class I Expression on the Growth of a Glioblastoma Cell Line
MHC class I complex genes constitute a highly polymorphic gene family which encodes transmembrane glycoproteins. They consist of a 45,000 dalton integral membrane protein noncovalently associated with the 12,000 dalton polypeptide, β2 microglobulin. MHC class I complex molecules appear to play an essential role in the presentation of tumor-specific antigens to the host’s immune system (reviewed in ). Recently, other roles of MHC class I have been documented, such as signal transduction and glucose transport. In the present experiment, we examined the influence of MHC class I expression on the growth of human glioma cells. We constructed a dexamethasone-inducible mouse MHC class I and introduced it into a human glioblastoma cell. Using this dexamethasone-inducible MHC class I-glioblastoma cell system, the influence of the expression of mouse MHC class I on anchorage-independent cell growth was compared between MHC class I expressing and non-expressing states.
KeywordsSoft Agar Human Glioblastoma Cell Human Glioblastoma Cell Line Sagum Medical School Murine Mammary Tumor Virus
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