In Situ Hybridization Using T-T Dimerized Non-Radioactive Probes

  • Paul K. Nakane
  • Hidekatsu Matsumura
  • Takehiko Koji


DNA labeled with non-radioactive markers have been used for in situ hybridization of specific DNA or RNA in cells and tissues. The presence of protruding markers on the probe DNA has been attributed to be the cause for the loss of sensitivity and specificity of hybridization. In search of a non-protruding marker, we investigated the possibility of the use of a T-T dimer which can be generated easily in DNA while being a potent hapten as a marker for DNA [1]. A T-T dimer in DNA was generated by UV irradiation (254 μm). Following hybridization with complementary DNA or RNA in cells and tissues, the T-T dimerized DNA (T-T DNA) was detected immunohistochemically using rabbit anti-T-T DNA and peroxidase-labeled goat anti-rabbit IgG. It was found that the use of T-T as a marker offers several advantages over other markers: it appears not to interfere with hybridization efficiency, is simple to make, and can be detected with high sensitivity.


HL60 Cell Electron Microscopic Level Yeast tRNA Sole Plasma Motor Endplate 
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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Paul K. Nakane
  • Hidekatsu Matsumura
  • Takehiko Koji
    • 1
  1. 1.Department of Anatomy IIINagasaki University School of MedicineNagasaki, 852Japan

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