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Oncogene Expression in High-Grade Astrocytomas: A Comparative Study of Immunogold-Silver and Peroxidase-Antiperoxidase Immunohistochemistry

  • Seiichi Yoshida
  • Ryuichi Tanaka
  • Andrew H. Kaye

Abstract

The expression products of many oncogenes is known to mediate cell proliferation [1–3], and much interest has been focused upon oncogene expression as a marker for neoplastic phenotypes [4]. Additionally, the level of expression of certain oncogenes in some tumors has been a significant indicator of biological behavior [5]. In this study, we examined the relative efficacy of immunogold silver (IGS) and peroxidase-antiperoxidase (PAP) immunohistochemistry in detecting the expression product of the epidermal growth factor receptor (EGF-R), c-myc, ras, and c-fos in 20 high-grade astrocytomas.

Keywords

Epidermal Growth Factor Receptor Epidermal Growth Factor Receptor Gene Expression Product Oncogene Expression Neoplastic Phenotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hermansson M, Nister M, Betsholtz C, et al. (1988) Endothelial cell hyperplasia in human glioblastoma: Coexpression of mRNA for platelet-derived growth factor B chain and PDGF receptor suggests autocrine growth stimilation. Proc Natl Acad Sci USA 85: 7748–7752PubMedCrossRefGoogle Scholar
  2. 2.
    Libermann TA, Razon AD, Bartal Y, et al. (1984) Expression of epidermal growth factor receptors in human brain tumors. Cancer Res 44: 753–760PubMedGoogle Scholar
  3. 3.
    Moelling KB, Heimann N, Beimling P, et al. (1984) Serine and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature 312: 558–561PubMedCrossRefGoogle Scholar
  4. 4.
    Thompson TC, Southgate J, Kitchener G, Land H (1989) Multistage carcinogenesis induced by ras and c-myc oncogenes in a reconstituted organ. Cell 56: 917–930PubMedCrossRefGoogle Scholar
  5. 5.
    Brodeur GM, Seeger RC, Shwab M, Varmus HE, et al. (1985) Amplification of the N-myc sequences in primary human neuroblastomas: Correlation with advanced disease stage. Prog Clin Biol Res 175: 105–113PubMedGoogle Scholar
  6. 6.
    Holgate CS, Jackson P, Cowen PN, et al. (1983) Immunogold silver staining: A new method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31: 983–984CrossRefGoogle Scholar
  7. 7.
    Filimus J, Pollak MN, Cairncross JG, et al. (1985) Amplified, overexpressed and rearranged epidermal growth factor receptor gene in a human astrocytoma cell line. Biochem Biophys Res Commun 131: 207–215CrossRefGoogle Scholar
  8. 8.
    Weinberg RA (1989) Oncogenes, antioncogenes and the molecular basis of multistep carcinogenesis. Cancer Res 49: 3713–3721PubMedGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Seiichi Yoshida
    • 1
  • Ryuichi Tanaka
    • 1
  • Andrew H. Kaye
    • 2
  1. 1.Department of Neurosurgery, Brain Research InstituteNiigata UniversityNiigata, 951Japan
  2. 2.Department of SurgeryMelbourne UniversityAustralia

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