Detection of Retinoblastoma Gene Aberrations in Human Brain Tumor by Single-Strand Conformation Polymorphism Analysis of Polymerase Chain Reaction Products
The gene predisposing to retinoblastoma, RB-1, has been mapped to chromo-somal region 13q14 , and a cDNA clone has been isolated . Aberrations of this gene have been found not only in retinoblastoma, but also in other human cancers, including osteosarcoma , small cell lung cancer , and bladder cancer . An epidemiological study revealed that the occurrence of brain tumor in members of a family with hereditary retinoblastoma was more frequent than in normal individuals . This observation suggested the involvement of aberrations of the RB gene in the genesis of brain tumor and prompted us to examine the gene in human brain tumors.
KeywordsGlioblastoma Cell Line Polymerase Chain Reac Human Brain Tumor Reverse Transcriptase Reaction Hereditary Retinoblastoma
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