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Detection of Retinoblastoma Gene Aberrations in Human Brain Tumor by Single-Strand Conformation Polymorphism Analysis of Polymerase Chain Reaction Products

  • Masako Katahira
  • Yoshinori Murakami
  • Kenshi Hayashi
  • Osami Kubo
  • Yasuhiko Tajika
  • Mizuo Kagawa
  • Takao Sekiya

Abstract

The gene predisposing to retinoblastoma, RB-1, has been mapped to chromo-somal region 13q14 [1], and a cDNA clone has been isolated [2]. Aberrations of this gene have been found not only in retinoblastoma, but also in other human cancers, including osteosarcoma [3], small cell lung cancer [4], and bladder cancer [5]. An epidemiological study revealed that the occurrence of brain tumor in members of a family with hereditary retinoblastoma was more frequent than in normal individuals [6]. This observation suggested the involvement of aberrations of the RB gene in the genesis of brain tumor and prompted us to examine the gene in human brain tumors.

Keywords

Glioblastoma Cell Line Polymerase Chain Reac Human Brain Tumor Reverse Transcriptase Reaction Hereditary Retinoblastoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Masako Katahira
    • 1
    • 2
  • Yoshinori Murakami
    • 2
  • Kenshi Hayashi
    • 2
  • Osami Kubo
    • 1
  • Yasuhiko Tajika
    • 1
  • Mizuo Kagawa
    • 1
  • Takao Sekiya
    • 2
  1. 1.Department of Neurosurgery, Neurological InstituteTokyo Women’s Medical CollegeShinjuku-ku, Tokyo, 162Japan
  2. 2.Oncogene DivisionNational Cancer Center Research InstituteChuo-ku, Tokyo 104Japan

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