The Expression and Structure of p53 Gene Products in Cultured Glioma Cells
Previous studies have demonstrated that the allelic deletions of the short arm of chromosome 17 (17p) and the long arm of 10 (10q) are closely associated with tumorigenesis of human malignant gliomas [1,2]. While the allelic deletion in chromosome 10q appears to occur in a relatively late phase associated with the transition from a less malignant to a more malignant state , the deletion in chromosome 17p seems to be an early event associated with the occurrence of neoplastic glial cells, suggesting that anti-oncogenes or tumor suppressor genes are important in the development of human gliomas. However, the role of anti-oncogenes in human gliomas has not been studied in detail. Recent studies have demonstrated that the cellular protein p53, which is encoded on chromosome 17p13.1, may function as a suppressor of neoplastic growth and may play an important role in the pathogenesis of human malignant tumors. The p53 protein was first identified through its interaction with the large T antigen of simian virus 40 (SV 40) , and had been thought to be a dominant oncogene enabling full transformation of vertebrate somatic cells in combination with an activated ras gene . However, it has recently been shown that the p53 protein observed in the neoplastic cells is entirely mutant and that the wild type p53 suppresses normal cells from transforming , which means that the p53 protein may act as a tumor suppressor, like the product of the retinoblastoma susceptibility gene.
KeywordsGlioma Cell Human Papilloma Virus T98G Cell Human Glioma Cell Line Al72 Cell
Unable to display preview. Download preview PDF.
- 1.El-Azouzi M, Chung RY, Farmer GE, Maltuza RL, Black PM, Rouleau GA, Hettlich C, Hedley-Whyte ET, Zervas NT, Panagopoulos K, Nakamura Y, Gusella JF, Seizinger BR (1989) Loss of distinct regions on the short arm of chromosome 17 associated with tumorigenesis of human astrocytomas. Proc Natl Acad Sci USA 86: 7186–7190PubMedCrossRefGoogle Scholar
- 15.Milner J, Cook A, Mason J (1990) p53 is associated with p34cdc2 in transformed cells. EMBO J 9: 2885–2889Google Scholar