Abstract
Restriction fragment length polymorphisms (RFLPs) analysis of human glioma tissue has revealed frequent loss of heterozygosity on the long arm of the chromosome 10 [1] as well as the short arm of the chromosome 17 [2,3]. It has recently been thought that certain tumor suppressor genes are located on chromosomes 10 and 17. The p53 gene is located on 17pl3.1, which is often deleted in various human tumors such as those of colorectal cancer, breast cancer, lung cancer, and osteosarcoma [4]. Detailed study of the p53 gene revealed that the remaining allele contained mutations particularly in the region conserved throughout the species [4]. It is reported that the product of a mutant-type p53 gene has a prolonged half-life and contains a cellular transforming activity. The p53 gene product acts in a dimer and the mutant-type p53 gene product inactivates the wild-type p53 gene product by binding to it [5]. While the mutant-type p53 gene causes cell immortalization in combination with the activated ras gene, the wild-type p53 gene product supresses normal somatic cell transformation. Thus, the genetic alterations of the p53 gene are thought to contribute to the tumorigenetic process in various type of cells, with the p53 gene being a putative tumor suppressor gene. Recent studies revealed that the p53 gene product may be acting as a transcriptional activator for some other genes [6,7]. This fact may well explain the diversity of tumor types which result from mutations in the p53 gene.
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References
Fujimoto M, Fults DW, Thomas GA, Nakamura Y, Heilbrun, MP, White R, Story JL, Naylor SL, Kagan-Hallet KS, Sheridan PJ (1989) Loss of hetrozygosity on chromosome 10 in human glioblastoma multiforme. Genomics 4: 210–214
James CD, Carlbom E, Nordenskjold M, Collons VP, Cavenee WK (1989) Mitotic recombination of chromosome 17 in astrocytomas. Proc Natl Acad Sci USA 86: 2858–2862
El-Azouzi M, Chung RY, Farmer GE, Martuza RL, Black PM, Rouleau GA, Hettlich C, Hedley-Whyte ET, Zervas NT, Panagopoulos K, Nakamura Y, Gusella JF, Seizinger BR (1989) Loss of distinct regions on the short arm of chromosome 17 associated with tumorigenesis of human astrocytomas. Proc Natl Acad Sci USA 86: 7186–7190
Nigro JM, Baker SJ, Preisinger AC, Jessup, JM, Hostetter R, Cleary K, Bigner SH, Davidson N, Baylin S, Devilee P, Glover T, Collins FS, Weston A, Medali R, Harris CC, Vogelstein B (1989) Mutations in the p53 gene occur in diverse human tumor types. Nature 342: 705–708
Levine AL, Momand J (1990) Tumor suppressor genes: The p53 and retinoblas-toma sensitivity genes and gene products. Biochim Biophys Acta 1032: 119–136
Field S, Jang SK (1990) Presence of potent transcription activating sequence in the p53 protein. Science 249: 1046–1049
Raycroft L, Wu H, Lozano G (1990) Transcriptional activation by wildtype but not transforming mutant of the p53 anti-oncogene. Science 249: 1049–1051
Orita M, Iwahara K, Kanazawa H, Hayashi K, Sekiya T (1989) Detection of polymorphisms of human DNA by gel electrophresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 86: 2766–2770
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor, New York
Buchman VL, Chumakov PM, Ninkina NN, Samarina OP, Georgiev GP (1988) A variation in the structure of the protein-coding region of human p53 gene. Gene 70: 245–252
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© 1991 Springer-Verlag Tokyo
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Fukuyama, K., Oh-uchida, M., Mineta, T., Momozaki, N., Hori, K., Tabuchi, K. (1991). An Analysis of Genetic Alterations of the p53 Gene in Human Glioma. In: Tabuchi, K. (eds) Biological Aspects of Brain Tumors. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68150-2_42
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DOI: https://doi.org/10.1007/978-4-431-68150-2_42
Publisher Name: Springer, Tokyo
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