Skip to main content
SpringerLink
Log in

Electrophoretical Analysis of DNA Topoisomerase I and II Expressions in Human Malignant Gliomas

  • Chapter
Book cover Biological Aspects of Brain Tumors

  • 78 Accesses

Abstract

DNA topoisomerases have been reported to play an important role in DNA replication and transcription [1]. They have been classified into two types of enzyme, topoisomerase I (Topo I) and topoisomerase II (Topo II). Topo I acts on single strand DNA in an adenosine triphosphate (ATP)-independent manner while Topo II acts on double strand DNA in the presence of ATP [2]. Topoisomerases appear to be essential for cell proliferation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Kuenzle CC (1985) Enzymology of DNA replication and repair in the brain. Brain Res Rev 10: 231–245

    Article  CAS  Google Scholar 

  2. Liu LF (1989) DNA Topoisomerase poisons as antitumor drugs. Annu Rev Biochem 58: 351–375

    Article  PubMed  CAS  Google Scholar 

  3. Tan KB, Mattern MR, Boyce RA, Schein PS (1987) Elevated DNA Topoisomerase II activity in nitrogen mustard-resistant human cells. Proc Natl Acad Sci USA 84: 7668–7671

    Article  PubMed  CAS  Google Scholar 

  4. Sklansky BD, Mann-Kaplan RS, Reynold AF, Rosenblum ML, Walker MD (1974) 4′-dementhyl-epipodophyllotoxin-β-D-thenylidene-glucoside ( PTG) in the treatment of malignant intracranial neoplasms. Cancer 33: 460–467

    Article  PubMed  CAS  Google Scholar 

  5. Labarca C, Paigen K (1980) A simple, rapid, and DNA assay procedure. Anal Biochem 102: 344–352

    Article  PubMed  CAS  Google Scholar 

  6. Wolff SN, Phillips GL, Herzig GP (1987) High-dose carmustine with autologous bone marrow transplantation for the adjuvant treatment of high-grade gliomas of the central nervous system. Cancer Treat Rep 71: 183–185

    PubMed  CAS  Google Scholar 

  7. Potmesil M, Hsiang YH, Liu LF, Bank B, Grossberg H, Kirschenbaum S, Forlen- zar TJ, Penziner A, Kanganis D, Knowles K, Traganos F, Silber R (1988) Resistance of human leukemic and normal lymphocytes to drug-induced DNA cleavage and low levels of DNA Topoisomerase II. Cancer Res 48: 3537–3543

    PubMed  CAS  Google Scholar 

  8. Hertzberg RP, Caranfa MJ, Hecht SM (1989) On the mechanism of Topoisomerase I inhibition by camptothecin: Evidence for binding to an enzyme-DNA complex. Biochemistry 28: 4629–4638

    Article  PubMed  CAS  Google Scholar 

  9. Hsiang YH, Liu LF, Wall ME, Wani MC, Nicholas AW, Manikumar G, Kirschen-baum S, Silber R, Potmesil M (1989) DNA Topoisomerase I-mediated DNA cleavage and cytotoxicity of camptothecin analogues. Cancer Res 49: 4385–4389

    PubMed  CAS  Google Scholar 

  10. Ling YH, Andersson BS, Nelson JA (1990) DNA Topoisomerase I as a site of action for 10-hydroxycamptothecin in human promyelocytic leukemia cells. Cancer Biochem Biophys 11: 23–30

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurological Surgery, Kagawa Medical School, Kita-gun, Kagawa, 761-07, Japan

    Takashi Ohmoto

  2. Department of Neurosurgery, The Johns Hopkins Hospital, USA

    Benjamin S. Carson

  3. Department of Pediatric Neurology, The Johns Hopkins Hospital, USA

    Peter C. Phillips

Authors
  1. Takahiro Tsuchida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshihito Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Noboru Sasaoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takashi Fujiwara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Ohmoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Benjamin S. Carson
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Peter C. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Saga Medical School, Nabeshima, Saga, 849, Japan

    Kazuo Tabuchi M. D. (Professor and Chairman) (Professor and Chairman)

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Tokyo

About this chapter

Cite this chapter

Tsuchida, T. et al. (1991). Electrophoretical Analysis of DNA Topoisomerase I and II Expressions in Human Malignant Gliomas. In: Tabuchi, K. (eds) Biological Aspects of Brain Tumors. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68150-2_38

Download citation

  • DOI: https://doi.org/10.1007/978-4-431-68150-2_38

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68152-6

  • Online ISBN: 978-4-431-68150-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation