Electrophoretical Analysis of DNA Topoisomerase I and II Expressions in Human Malignant Gliomas

  • Takahiro Tsuchida
  • Yoshihito Matsumoto
  • Noboru Sasaoka
  • Takashi Fujiwara
  • Takashi Ohmoto
  • Benjamin S. Carson
  • Peter C. Phillips


DNA topoisomerases have been reported to play an important role in DNA replication and transcription [1]. They have been classified into two types of enzyme, topoisomerase I (Topo I) and topoisomerase II (Topo II). Topo I acts on single strand DNA in an adenosine triphosphate (ATP)-independent manner while Topo II acts on double strand DNA in the presence of ATP [2]. Topoisomerases appear to be essential for cell proliferation.


Malignant Glioma Johns Hopkins Hospital Nitrogen Mustard Human Malignant Glioma Cleavable Complex 
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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Takahiro Tsuchida
  • Yoshihito Matsumoto
  • Noboru Sasaoka
  • Takashi Fujiwara
  • Takashi Ohmoto
    • 1
  • Benjamin S. Carson
    • 2
  • Peter C. Phillips
    • 3
  1. 1.Department of Neurological SurgeryKagawa Medical SchoolKita-gun, KagawaJapan
  2. 2.Department of NeurosurgeryThe Johns Hopkins HospitalUSA
  3. 3.Department of Pediatric NeurologyThe Johns Hopkins HospitalUSA

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